Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants

Kaur, Navdeep; Sharma, Shubham; Hasanuzzaman, Mirza; Pati, Pratap Kumar

doi:10.1155/2022/5547231

Cited by 19 publications

(5 citation statements)

References 141 publications

(169 reference statements)

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“…The chloroplast of Chlamydomonas reinhardtii, a unicellular alga, was found to contain I-CreI, which is found in the 23S rRNA gene. However, due to the difficulties in reengineering meganucleases to target specific DNA areas, their utility in genome editing is limited [63].…”

Section: Meganucleasesmentioning

confidence: 99%

Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants

Yadav

Tripathi

Tiwari

et al. 2023

Life

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Genome editing aims to revolutionise plant breeding and could assist in safeguarding the global food supply. The inclusion of a 12–40 bp recognition site makes mega nucleases the first tools utilized for genome editing and first generation gene-editing tools. Zinc finger nucleases (ZFNs) are the second gene-editing technique, and because they create double-stranded breaks, they are more dependable and effective. ZFNs were the original designed nuclease-based approach of genome editing. The Cys2-His2 zinc finger domain’s discovery made this technique possible. Clustered regularly interspaced short palindromic repeats (CRISPR) are utilized to improve genetics, boost biomass production, increase nutrient usage efficiency, and develop disease resistance. Plant genomes can be effectively modified using genome-editing technologies to enhance characteristics without introducing foreign DNA into the genome. Next-generation plant breeding will soon be defined by these exact breeding methods. There is abroad promise that genome-edited crops will be essential in the years to come for improving the sustainability and climate-change resilience of food systems. This method also has great potential for enhancing crops’ resistance to various abiotic stressors. In this review paper, we summarize the most recent findings about the mechanism of abiotic stress response in crop plants and the use of the CRISPR/Cas mediated gene-editing systems to improve tolerance to stresses including drought, salinity, cold, heat, and heavy metals.

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Section: Meganucleasesmentioning

confidence: 99%

Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants

Yadav

Tripathi

Tiwari

et al. 2023

Life

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“…Genetic engineering approaches based on the insertion of a transgene are restricted in certain areas of the world. Thus, to supply to the world’s rising population, there is great hope in genome editing tools, which are expected to revolutionize the world’s agriculture [ 121 ]. In tomato, genome editing has been performed for different genes, and resistance to abiotic stress has been obtained.…”

Section: Genome Editing For Ameliorating Abiotic Stresses In Tomatomentioning

confidence: 99%

Biomimetic Strategies for Developing Abiotic Stress-Tolerant Tomato Cultivars: An Overview

Kumar

Choudhary

et al. 2022

Plants

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The tomato is one of the most important vegetables in the world. The demand for tomatoes is high in virtually any country, owing to their gastronomic versatility and nutritional and aromatic value. Drought, salinity, and inadequate temperature can be major factors in diminishing yield, affecting physiological and biochemical processes and altering various metabolic pathways, from the aggregation of low molecular–weight substances to the transcription of specific genes. Various biotechnological tools can be used to alter the tomato genes so that this species can more rapidly or better adapt to abiotic stress. These approaches range from the introgression of genes coding for specific enzymes for mitigating a prevailing stress to genetic modifications that alter specific metabolic pathways to help tomato perceive environmental cues and/or withstand adverse conditions. In recent years, environmental and social concerns and the high complexity of the plant response may increase the attention of applied plant biotechnology toward biomimetic strategies, generally defined as all the approaches that seek to develop more sustainable and acceptable strategies by imitating nature’s time-tested solutions. In this review, we provide an overview of some of the genetic sequences and molecules that were the objects of biotechnological intervention in tomato as examples of approaches to achieve tolerance to abiotic factors, improving existing nature-based mechanisms and solutions (biomimetic biotechnological approaches (BBA)). Finally, we discuss implications and perspectives within the GMO debate, proposing that crops modified with BBA should receive less stringent regulation.

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“…The field of biotechnology is at a turning point due to a reduction in the cost and surge in the speed of DNA synthesis and sequencing, making it practically possible to change certain base pairs of a particular genome. , Till now, meganucleases, zinc finger, TALENs, and CRISPR/Cas have been used for editing the genomes of different organisms ranging from bacteria to humans. − Amid these, CRISPR/Cas is the most researched approach and has significantly remodeled basic and applied biological research, so the past decade is often regarded as the CRISPR era . However, to edit DNA to produce desired mutations, CRISPR/Cas cascade has to locate and cut specific portions of DNA followed by deceiving the cell into utilizing a new piece of DNA to patch up the break .…”

Section: Introductionmentioning

confidence: 99%

Retron Library Recombineering: Next Powerful Tool for Genome Editing after CRISPR/Cas

Kaur,

Pati

2024

ACS Synth. Biol.

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Retron library recombineering (RLR) is a powerful tool in the field of genome editing that exceeds the scope and specificity of the CRISPR/Cas technique. In RLR, singlestranded DNA produced in vivo by harnessing the in-built potential of bacterial retrons is used for replication-dependent genome editing. RLR introduces several genomic variations at once, resulting in pooled and barcoded variant libraries, thus permitting multiplexed applications. Retron-generated RT-DNA has already shown promise for use in genome editing. Thus, this new tool will result in fresh, intriguing, and surprising developments in molecular biology and its juncture with other disciplines of research, including medicine, agriculture, and microbiology. In this review, we discuss the current state of this brand-new tool that could eventually boost genome editing research.

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Genome Editing: A Promising Approach for Achieving Abiotic Stress Tolerance in Plants

Cited by 19 publications

References 141 publications

Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants

Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants

Biomimetic Strategies for Developing Abiotic Stress-Tolerant Tomato Cultivars: An Overview

Retron Library Recombineering: Next Powerful Tool for Genome Editing after CRISPR/Cas

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