<i>Sarcocystis</i> spp. in Romanian Slaughtered Cattle: Molecular Characterization and Epidemiological Significance of the Findings

Imre, K.; Dărăbuş, G.; Tîrziu, E.; Morariu, Sorın; Imre, M.; Plutzer, Judit; Boldea, Marius; Morar, Adriana

doi:10.1155/2019/4123154

Cited by 14 publications

(19 citation statements)

References 27 publications

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“…Sarcocystis was also detected in cattle heart at prevalence rates of 100% and 17.9% from Brazil and Romania by Ferreira et al [36] and Imre et al [37], respectively.…”

Section: Discussionmentioning

confidence: 87%

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Abdullah

2021

Vet World

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Background and Aim: Sarcocystosis is a zoonotic infection caused by various species of Sarcocystis organisms with a worldwide geographic distribution. This study investigated the presence of Sarcocystis organisms in cattle and sheep slaughtered at an abattoir in Sulaimani Province in North Iraq. Materials and Methods: A total of 130 muscle samples were collected during May, June, and July of 2020, including 80 samples from sheep and 50 samples from cattle. Samples were examined visually for macrosarcocysts. The peptic digestion method was used to analyze fresh muscle tissue samples for detecting microsarcocysts followed by microscopic examination. Furthermore, muscle samples were fixed and stained with hematoxylin and eosin for histopathological examination. Results: In the gross examination, macroscopic cysts were not detected in both cattle and sheep; hence, all the prevalence data were obtained through microscopic observation of muscle samples. The peptic digestion method revealed the presence of banana-shaped bradyzoites in 90% and 92.5% of slaughtered cattle and sheep muscle samples, respectively. Organ-wise prevalence revealed that 95% and 92% of esophageal samples of sheep and cattle contained Sarcocystis spp., respectively. Moreover, 90% and 88% of sheep and cattle diaphragms were respectively infected. Histopathological examination of tissue sections revealed two morphologically distinct types of microsarcocysts, including thin-walled and thick-walled, in both sheep and cattle. Conclusion: The suspected Sarcocystis spp. were Sarcocystis tenella and Sarcocystis arieticanis in sheep and Sarcocystis cruzi and Sarcocystis bovifelis or Sarcocystis hominis in cattle. Infective stages of different Sarcocystis spp. are widespread in the study area environment.

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“…Sarcocystis was also detected in cattle heart at prevalence rates of 100% and 17.9% from Brazil and Romania by Ferreira et al [36] and Imre et al [37], respectively.…”

Section: Discussionmentioning

confidence: 87%

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Abdullah

2021

Vet World

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“…Nevertheless, several trends in the prevalence of Sarcocystis species can be indicated. Most studies carried out in cattle reported the highest prevalence of S. cruzi employing canids as definitive hosts [10,14,15,18,37,[40][41][42][43][44]. By contrast, a relatively low prevalence of S. hirsuta using felids as definitive hosts was often recorded [14,15,37,41,45].…”

Section: Discussionmentioning

confidence: 99%

“…Most studies carried out in cattle reported the highest prevalence of S . cruzi employing canids as definitive hosts [ 10 , 14 , 15 , 18 , 37 , 40 – 44 ]. By contrast, a relatively low prevalence of S .…”

Section: Discussionmentioning

confidence: 99%

“…from cattle have been molecularly characterised at the genes for 18S rRNA ( 18S rRNA), 28S rRNA ( 28S rRNA) and cytochrome c oxidase subunit I ( cox 1) and at nuclear rDNA internal transcribed spacer 1 (ITS1) [ 8 , 10 , 11 , 15 ]. Databases contain many 18S rRNA gene sequences of the genus Sarcocystis used for species identification [ 16 – 18 ]. However, studies have revealed that cox 1 is the preferable target to differentiate taxonomically related Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

et al. 2020

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Background Six Sarcocystis species are known to use cattle (Bos taurus) as the intermediate host, two of which, S. hominis and S. heydorni, are zoonotic. There is a need for a method that will enable rapid identification of the Sarcocystis species in cattle. Methods The diaphragm muscles of 102 cattle from Lithuania were examined for the presence of Sarcocystis spp., using two different methods for species identification. Individual sarcocysts were isolated from squash preparations of the diaphragm muscle under the light microscope, followed by genetic characterisation of excised cysts using sequence analysis of the 18S rRNA (18S rRNA) and cytochrome c oxidase subunit I (cox1) genes. The same cattle muscle samples were digested and species-specific PCR analyses targeting cox1 were developed to identify the Sarcocystis isolates to the species level. Results Under the light microscope, sarcocysts were detected in 87.3% of animals, and Sarcocystis infection was verified in all digested samples. Three species, namely S. cruzi (n = 20), S. bovifelis (n = 23) and S. hirsuta (n = 6), were identified by DNA sequence analysis of isolated sarcocysts. Based on sequence analysis of cox1, the level of genetic variability depended on Sarcocystis species and geographical location. Four Sarcocystis species, S. cruzi (96.1%), S. bovifelis (71.6%), S. hirsuta (30.4%) and S. hominis (13.7%), were confirmed in the digested samples. In individual samples, the most common finding was two species of Sarcocystis (44.1%), followed by three species (26.5%), a single species (24.5%) and four species (4.9%). Conclusions Although examination of tissue preparations under the light microscrope did not detect any sarcocysts belonging to S. hominis, this species was identified in the digested samples subjected to a cox1-specific PCR analysis. These results demonstrate the need for effective molecular diagnosis techniques to detect Sarcocystis spp., which may be present at a lower prevalence and not detectable among the limited number of sarcocysts identified individually under the light microscope.

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“…The definitive hosts of these species are canids (S. cruzi), felids (S. hirsuta, S. bovini, S. bovifelis and S. rommeli) and humans (S. hominis and S. heydorni). It has been found that among these species S. hominis and S. heydorni caused zoonotic infections, infected humans with infected pork and beef or contaminated food, and calves were infected with sporocysts excreted in feces as a result of infection (6,10,15,18).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey

Oğuz

Değer

Koşal

2021

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.

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Sarcocystis spp. in Romanian Slaughtered Cattle: Molecular Characterization and Epidemiological Significance of the Findings

Cited by 14 publications

References 27 publications

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey

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