<i>Salmonella enterica mcr-1</i> Positive from Food in Brazil: Detection and Characterization

Rau, Renata Batista; Lima-Morales, Daiana de; Wink, Priscila Lamb; Ribeiro, A. R.; Barth, Afonso Luís

doi:10.1089/fpd.2019.2700

Cited by 30 publications

(26 citation statements)

References 34 publications

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“…Up to now, the mcr-1 gene is the most frequent plasmid-mediated colistin-resistance gene reported in Brazil 26 , 27 . It has been described in Salmonella enterica isolated from poultry meat 21 , from food 27 , and in E. coli from cattle and humans 28 , 29 and Klebsiella pneumoniae from humans 29 .…”

Section: Discussionmentioning

confidence: 99%

Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections

Paiva

Nagano

Cotia

et al. 2021

Rev. Inst. Med. trop. S. Paulo

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Section: Discussionmentioning

confidence: 99%

Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections

Paiva

Nagano

Cotia

et al. 2021

Rev. Inst. Med. trop. S. Paulo

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“…Plasmid transferability was evaluated by conjugation experiments using E. coli J53 (J53AZR). Each mcr-1 –positive isolate was incubated with E. coli J53 on a cellulose membrane in a Luria–Bertani agar plate ( Rau et al., 2020 ). After incubation at 37°C for 24 h, POL-resistant transconjugants were selected on Luria–Bertani agar plates containing sodium azide (100 μg/mL) and POL (0.5 μg/mL).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

Lin

et al. 2020

Poultry Science

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Multidrug-resistant ( MDR ) Escherichia coli are responsible for difficult-to-treat infections. We sought to determine the prevalence and characteristics of MDR E. coli strains isolated from poultry and clinical patients in the same geographical region. Eighty-seven E. coli strains were isolated from poultry with perihepatitis lesions at different slaughterhouses, and 356 nonrepetitive E. coli strains were isolated from clinical patients. All samples were continuously collected from October to December 2017 in Tai'an, China. The presence of the mcr-1 gene in the strains was assessed by PCR. The genetic relationships of the polymyxin ( POL )-resistant E. coli strains were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing. The results indicate that the POL resistance rate for the E. coli isolates from poultry was 31.03% (27 of 87), whereas the human-origin E. coli isolates were 100% sensitive to POL. The mcr-1 gene and extended-spectrum β-lactamase bla CTX-M-14 genes were identified in all 27 POL-resistant avian-origin E. coli isolates. Our pulsed-field gel electrophoresis analysis suggested that the 27 strains were represented by 14 pulsotypes, among which there were 3 strains each with A, E, I, and K pulsotypes, and 1 to 2 strains represented by the other 10 pulsotypes. Furthermore, multilocus sequence typing molecular typing identified 16 sequence types, including 4 ST156 strains, 3 ST533 strains, and 1 to 2 strains represented by the remaining 14 sequence types. In summary, the E. coli strains isolated in the Tai'an area all showed the MDR phenotype, the rate of which for poultry was higher than that for humans. No POL-resistant human-origin E. coli strains were identified in the clinical patients. Our study reveals that poultry-derived MDR mcr-1 –positive E. coli strains may pose a potential risk to humans, and the surveillance findings presented herein will be conducive to our understanding of the prevalence and characteristics of mcr-1 –positive E. coli strains in the Tai'an area.

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“…As in other countries, the colistin was extensively used in Brazil as a growth promoter for many years. In 2016, the government published restrictions on the use of colistin in animal production (1,26), which came into force in 2018. However, the use of colistin to treat or prevent infections in veterinary medicine including animal productions is still allowed.…”

Section: Mcr-1 In Latin Americamentioning

confidence: 99%

“…Although the mobility and dissemination of the mcr-1 gene are associated with ISApl1 and the pap2 gene in most plasmid types (43), the genetic context of the IncX4 plasmid type, in Latin America, is different. This context is characterized by lacking the ISApl1, but it preserves the pap2 sequence and a hypothetical protein (hp) around the mcr-1 gene (26,44). What would be the explanation for that?…”

Section: Genetic Context and Dissemination Of Mcr-1 Genementioning

confidence: 99%

“…Furthermore, the IS26 mobile element upstream to the mcr-1 gene has been also associated with IncX4 plasmid types in Brazil, but there are no other reports in Latin America (26,46) (Table 1). This Insertion Sequence (IS) plays an important role in the dissemination and evolution of the antimicrobial resistance genes on plasmids, including colistin resistance genes (1).…”

Section: Genetic Context and Dissemination Of Mcr-1 Genementioning

confidence: 99%

See 1 more Smart Citation

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

Lentz

Dalmolin

Barth

et al. 2021

Front. Public Health

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Salmonella enterica mcr-1 Positive from Food in Brazil: Detection and Characterization

Cited by 30 publications

References 34 publications

Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections

Colistin-resistant Escherichia coli belonging to different sequence types: genetic characterization of isolates responsible for colonization, community- and healthcare-acquired infections

Prevalence of avian-origin mcr-1–positive Escherichia coli with a potential risk to humans in Tai'an, China

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

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