<i>Saccharomyces cerevisiae</i> Mer2, Mei4 and Rec114 Form a Complex Required for Meiotic Double-Strand Break Formation

Li, Jing; Hooker, Gillian W.; Roeder, G. Shirleen

doi:10.1534/genetics.106.058768

Cited by 114 publications

(211 citation statements)

References 45 publications

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“…3 and data not shown). These findings differ from those of Li et al (2006), who found that chromosomal association of a different tagged version of Mei4 (Mei4-GFP) was unaffected by rec114 mutation and only slightly reduced in a mer2 mutant. Possible reasons for these differences are addressed in the Discussion.…”

Section: Genetic Requirements For Chromosome Associationcontrasting

confidence: 99%

“…These results agree with earlier findings by Li et al (2006). In contrast, normal subcellular localization of Mei4 was dependent on the presence of Rec114 and Mer2.…”

Section: Genetic Requirements For Chromosome Associationsupporting

confidence: 93%

“…While this work was in progress, an independent study demonstrated that Mei4 and Rec114 localize to chromosomes in discrete foci or patches, and that the extent of staining for both proteins declines with progression through prophase (Li et al 2006). This earlier study was conducted with different tagged versions of these proteins (Rec114myc3 and Mei4-GFP) and in a different strain background (BR).…”

Section: Association Of Mei4 and Rec114 With Meiotic Chromosomesmentioning

confidence: 95%

“…The MER2 message is constitutively expressed but contains an intron that is spliced efficiently only in the presence of a meiosis-specific splicing factor, Mer1 (Engebrecht et al 1991;Nandabalan and Roeder 1995;Spingola and Ares 2000). As a result of this regulation, Mer2 protein is present at low levels during vegetative growth and at substantially higher levels during meiosis (Henderson et al 2006;Li et al 2006). …”

Section: Introductionmentioning

confidence: 99%

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Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but roles of these proteins and interactions among them are poorly understood. We report here further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11.

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Section: Genetic Requirements For Chromosome Associationcontrasting

confidence: 99%

“…These results agree with earlier findings by Li et al (2006). In contrast, normal subcellular localization of Mei4 was dependent on the presence of Rec114 and Mer2.…”

Section: Genetic Requirements For Chromosome Associationsupporting

confidence: 93%

Section: Association Of Mei4 and Rec114 With Meiotic Chromosomesmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

et al. 2007

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“…20 The function of LOC105370891 has not been studied in detail. Neuroplastin encodes a type I transmembrane protein belonging to the immunoglobulin superfamily; it may be involved in cell-cell interactions or cell-substrate interactions.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide association study using the ethnicity-specific Japonica array: identification of new susceptibility loci for cold medicine-related Stevens–Johnson syndrome with severe ocular complications

et al. 2017

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A genome-wide association study (GWAS) for cold medicine-related Stevens-Johnson syndrome (CM-SJS) with severe ocular complications (SOC) was performed in a Japanese population. A recently developed ethnicity-specific array with genome-wide imputation that was based on the whole-genome sequences of 1070 unrelated Japanese individuals was used. Validation analysis with additional samples from Japanese individuals and replication analysis using samples from Korean individuals identified two new susceptibility loci on chromosomes 15 and 16. This study might suggest the usefulness of GWAS using the ethnicity-specific array and genome-wide imputation based on large-scale whole-genome sequences. Our findings contribute to the understanding of genetic predisposition to CM-SJS with SOC.

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Current awareness on yeast

2007

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (6 weeks journals ‐ search completed 7th. Feb. 2007)

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Saccharomyces cerevisiae Mer2, Mei4 and Rec114 Form a Complex Required for Meiotic Double-Strand Break Formation

Cited by 114 publications

References 45 publications

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae

Genome-wide association study using the ethnicity-specific Japonica array: identification of new susceptibility loci for cold medicine-related Stevens–Johnson syndrome with severe ocular complications

Current awareness on yeast

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