RHD positive among C/E+ and D− blood donors in Denmark

Christiansen, Mette; Sørensen, B.; Grunnet, Niels

doi:10.1111/j.1537-2995.2010.02611.x

Cited by 24 publications

(29 citation statements)

References 14 publications

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“…The Rh antigens are also the most clinically significant in transfusion medicine [1,2]. They are located on two proteins, RhD and RhCE: the former carries the D antigen, while the latter carries C, c, E, and e antigens.…”

Section: Brief Communication (Original)mentioning

confidence: 99%

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RH genotypes among Malaysian blood donors

Musa

Hassan²,

Ayob³

et al. 2014

Asian Biomedicine

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Background: RH genotyping studies have been conducted mainly in people of Caucasian and African descent. There is limited information regarding the molecular basis for RH genotypes in Malaysia. Objectives: To investigate the prevalence and characteristics of RHCE genotypes among different ethnic groups in Malaysia. Methods: A total of 1014 whole blood samples were obtained from donors from 4 different ethnic groups (360 Malays, 434 Chinese, 164 Indians, and 56 others). All samples were phenotyped for C, c, D, E, and e using standard serologic methods and genotyped using polymerase chain reaction (PCR)-based analysis. Results:In the blood samples that we analyzed, the distribution of RH genotype antigens was significantly different among the various ethnic groups. Our findings showed that CCDee is the most common in Malaysian blood donors; 18.4% (187/1014) compared with other genotypes. The ccDEE genotype is more prevalent in the Chinese: 65.6% (82/125), and the ccee genotype is more prevalent in Indians: 47.1% (65/138). There were discrepancies between phenotypes and genotypes. There were 17 (1.7%) discrepancies in RH C/c genotyping results and of these 47% (8/17) occurred in Malays. Discrepancies in RH E/e results occurred in 3 samples (0.3%). Conclusions: Our study provides a database for the distribution of RH genotypes of donors from the major ethnic groups in Malaysia. Methods used in this study are useful for comparing the phenotypes and genotypes. Further investigation should be conducted to study the causes of these discrepancies using other molecular based techniques.

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Section: Brief Communication (Original)mentioning

confidence: 99%

“…Several RH D, RH C/c, and RH E/e genotyping assays have been developed. A number of studies have been carried out to assess the frequency of these three molecular backgrounds in people of African and Caucasian ancestry [2,8].…”

Section: Brief Communication (Original)mentioning

confidence: 99%

RH genotypes among Malaysian blood donors

Musa

Hassan²,

Ayob³

et al. 2014

Asian Biomedicine

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“…Although the main RHD alleles resulting in a D- phenotype, i.e. RHDΨ and (C)ce s , are most frequently cis -associated with RHCE*ce , other RHD gene variants in D- individuals, including several hybrid genes, have been shown to segregate with RHCE alleles expressing C and/or E (C/E+) antigens in Caucasian, Asian, African, and mixed populations [4,7,8,9,10,11,12,13,14,15,16,17,18,19,20]. …”

Section: Introductionmentioning

confidence: 99%

RHD-Positive Alleles among D- C/E+ Individuals from India

Kulkarni

Gogri

Parchure

et al. 2018

Transfus Med Hemother

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Background: Molecular bases of blood group systems, including Rh blood group, have been poorly studied in the Indian population so far, while specificities of Europeans, East Asians and Africans have been well known for years. In order to gain insights into the molecular bases of this population, we sought to characterize the RHD allele in D- Indian donors expressing C and/or E antigen(s). Methods:RHD gene was analyzed in 171 serologically D-, C/E+ samples by standard molecular methods such as quantitative, multiplex PCR of short fluorescent fragments (QMPSF) and direct sequencing when necessary. Results:RHD whole gene deletion at the homozygous state was found to be the most common genotype associated with D- phenotype (118/171, 69.0%). Nonfunctional, negative hybrid genes with reported molecular backgrounds were observed in approximately one-third of the samples, while only four samples carry single-nucleotide variations, including one novel nonsense (RHD(Y243X)), one novel frameshift (RHD(c.701delG)), and two missense (RHD(T148R) and RHD(T148R, T195M)) alleles. Conclusion: Overall we report for the first time the molecular bases of D antigen negativity in the D-, C/E+ Indian population, which appears to be qualitatively similar to other populations, but with a population-specific, quantitative distribution of D-- alleles.

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“…Generally, the frequency of DEL among Europeans is 1:350 to 1:2,000, while it gets as high as 1:5 among Asians. It should be noted that in many studies [8,23,47,56] weak D or partial D donors were detected among donors previously believed to be D-negative.…”

Section: Frequency Of Missed D-positive Samplesmentioning

confidence: 99%

“…Since most DEL and weak D differ from normal RHD by splice site or missense mutations, they are not affected by this problem, and testing for almost any RHD -specific polymorphism will reveal the vast majority of DEL samples. Therefore, if sensitive serologic testing is continued to detect partial D caused by hybrid alleles, testing a single RHD polymorphism by PCR may be a possible choice to find the DEL samples [28,35,47]. …”

Section: Which Rhd Pcr Should Be Used?mentioning

confidence: 99%

RHD PCR of D-Negative Blood Donors

Wagner¹

2013

Transfus Med Hemother

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RHD PCR of blood donors may be used to reveal weak D, partial D, DEL and chimeric D+/D- donors among presumed D-negative blood donors. Units donated by such donors pose a definite yet low risk for anti-D immunization of transfusion recipients. The frequency of DEL donors among D-negative donors is 1:350 to 1:2,000 in Europe and up to 1:5 in Asian countries. Different strategies for RHD PCR of blood donors have been used. Probably, the most cost-efficient implementation is replacement of sensitive D antigen testing with the indirect antiglobulin test by RHD PCR in pools which might even reduce total testing cost.

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RHD positive among C/E+ and D− blood donors in Denmark

Cited by 24 publications

References 14 publications

RH genotypes among Malaysian blood donors

RH genotypes among Malaysian blood donors

RHD-Positive Alleles among D- C/E+ Individuals from India

<b><i>RHD</i></b> PCR of D-Negative Blood Donors

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