Retracted: miR‐150 might inhibit cell proliferation and promote cell apoptosis by targeting LMO4 in Burkitt lymphoma

Abstract

This study aimed at investigating the effect of microRNA‐150 (miR‐150) on cell proliferation of Burkitt lymphoma and its molecular mechanism. Gene expression analysis was applied to identify target genes of miR‐150 in Burkitt lymphoma cell line ST486 based on the dataset from the Gene Expression Omnibus (GEO) datasets GSE86432. miRNA mimics, inhibitor and small interfering RNA (siRNA) were fluorescently labeled by Cy3, whereas plasmid vector was labeled by EGFP. Cells were viewed by fluorescence microscope and transfection efficiency was evaluated through fluorescent cell percentage. Quantitative real‐time polymerase chain reaction analysis (qRT‐PCR) and western blot were applied to detect the expression level of miR‐150 and LMO4. Cell proliferation, cell cycle, and apoptosis were explored by CCK‐8, flow cytometry. Targeting relationship was validated by the Luciferase reporter assay. Tumor xenograft and immunohistochemical analysis were conducted in nude mice model. In Burkitt lymphoma cells, miR‐150 expression was significantly lower than normal ones, whereas the expression of LMO4 was upregulated. miR‐150 might inhibit cell proliferation and promoted apoptosis in Burkitt lymphoma deterioration by downregulating LMO4. The results of tumor xenograft further confirmed the role of miR‐150 in Burkitt lymphoma. Targeting LMO4 is a significant mechanism by which miR‐150 suppresses cell growth and promotes apoptosis in Burkitt lymphoma cells, thus may provide a novel target for Burkitt lymphoma therapy in the future.

Curcumin in Combination With Omacetaxine Suppress Lymphoma Cell Growth, Migration, Invasion, and Angiogenesis via Inhibition of VEGF/Akt Signaling Pathway

“…Moreover, evidences have shown that VEGFA expression can be regulated by various microRNAs (miRNAs), including of miR-150, miR-22, miR-206 and miR-34a ( 33 – 36 ). Meanwhile, the levels of miR-150, miR-22, miR-206 and miR-34a have been found to be downregulated in human cancers ( 37 – 40 ). Thus, we investigated whether HHT combined with curcumin reduced VEGFA level in Raji cells by these miRNAs.…”

Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-related Penile Squamous Cell Carcinoma

“…TP63 and LMO4 have been intensively studied as transcriptional targets in squamous cell carcinoma. They can regulate the expression of diverse tumor-related proteins and are involved in extracellular matrix and tumor microenvironment remodeling as well as in growth factor-mediated signal transduction [30,31,32]. Pathological specimens of HIV-related penile squamous cell carcinoma are very rare, so our sample size is relatively small, but the results obtained still have certain reference value.…”

Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-Related Penile Squamous Cell Carcinoma

“…TP63 and LMO4 have been intensively studied as transcriptional targets in squamous cell carcinoma. They can regulate the expression of diverse tumor-related proteins and are involved in extracellular matrix and tumor microenvironment remodeling as well as in growth factor-mediated signal transduction Zhang, 2019). Among the KEGG pathways, two terms linked to the regulation of cell proliferation and survival, including DNA replication and cell adhesion molecule metabolism, were identified.…”