Retracted: MicroRNA‐150‐5p affects cell proliferation, apoptosis, and EMT by regulation of the BRAFV600E mutation in papillary thyroid cancer cells

Yan, Ruihong; Yang, Tianzheng; Zhai, Hongyan; Zhou, Zhonghai; Gao, Lei; Li, Yuhong

doi:10.1002/jcb.27108

Cited by 31 publications

(23 citation statements)

References 32 publications

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“…In a previous study, a miRNA array of circulating plasma extracellular vesicles from CLP-induced septic mice revealed that the expression of miR-150-5p increased significantly compared to control mice, thus affecting inflammation and the production of inflammatory cytokines and suggesting that miR-150-5p in the myocardial tissue may be differentially expressed [ 23 ]. In addition, Yan et al found that miR-150-5p could inhibit apoptosis by regulating the mutation site V600E of the gene BRAF in papillary thyroid cancer cells [ 24 ]. The above findings are consistent with the findings of our current study.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Zhu

Zhang

Wen

et al. 2020

BioMed Research International

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Sepsis-induced myocardial depression has high mortality and is very common in intensive care units. Previous studies have found that microRNAs play an important role in regulating sepsis-induced myocardial depression. miR-150-5p is involved in many biological processes; however, the mechanism underlying its role in sepsis-induced myocardial depression is still unclear. In this study, we generated rat models of septic shock induced by lipopolysaccharide. Whole genomic RNA sequencing was performed on 12 left ventricles collected after LPS treatment to identify miRNAs. Most of the target genes of the differently expressed microRNAs were involved in apoptosis, according to Gene Ontology. We also observed apoptosis in the heart tissue and in H9C2 cardiomyocytes stimulated with lipopolysaccharide, indicating that cell apoptosis may be an important mechanism in sepsis-induced myocardial depression. Furthermore, the expression of miR-150-5p was reduced, and overexpression of miR-150-5p with mimics resulted in a decrease in apoptosis, decreased expression of cleaved caspase3 and Bax, and increased expression of Bcl-2. Additionally, after H9C2 cells were transfected with miR-150-5p mimics or an inhibitor, the expression of Akt2 decreased or increased, respectively. These findings suggest that miR-150-5p can alleviate apoptosis and may be a novel therapeutic target for sepsis-induced myocardial depression.

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Section: Discussionmentioning

confidence: 99%

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Zhu

Zhang

Wen

et al. 2020

BioMed Research International

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“…Numerous experiments have found that miRNAs were involved in all aspects of tumor-related processes, including proliferation, apoptosis, metastasis, angiogenesis, and immune response. [4][5][6] Because of its high sensitivity and specificity, miRNA is very likely to become a new type of tumor diagnostic and prognostic markers and a new target for tumor treatment. 7,8 Therefore, it is meaningful to study the miRNAs differentially expressed (DE-miRNAs) in GBM including in serum or tumor tissues.…”

Section: Introductionmentioning

confidence: 99%

Reduced Expression of hsa-miR-338-3p Contributes to the Development of Glioma Cells by Targeting Mitochondrial 3-Oxoacyl-ACP Synthase (OXSM) in Glioblastoma (GBM)

Wang¹,

Lu²

2020

OTT

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Background: MicroRNAs have been identified as major regulators and therapeutic targets of glioblastoma (GBM). It is thus meaningful to study the miRNAs differentially expressed (DE-miRNAs) in GBM. Materials and Methods: We performed a meta-analysis of previously published microarray data using the R-based "metaMA" package to identify DE-miRNAs.The biological processes of the DE-miRNAs were then analyzed using FunRich. KEGG pathways of the DE-miRNAs gene targets were analyzed by mirPath V.3. Luciferase activity assay was performed to validate that OXSM is a direct target of hsa-miR338-3p. Flow cytometry was used to detect the effects of miR-338-3p on GBM cell proliferation, apoptosis and cell cycle. Results: DE-miRNAs in blood and brain tissue from GBM were identified. "Type I interferon signaling pathway" and "VEGF and VEGFR signaling network" were the most significantly enriched biological processes shared by all GBM types. In KEGG pathway analysis, DE-miRNAs both in blood and tissue show altered fatty acid biosynthesis. Further validation shows hsa-miR-338-3p regulates fatty acid metabolism by directly targeting OXSM gene. In addition, our data revealed an accelerated cell cycle and an anti-apoptotic role for OXSM in glioma cells, which has not been reported. Finally, we confirmed that hsa-miR-338-3p inhibitor antagonized the effect of downregulation of OXSM on cell cycle and apoptosis of GBM cells. Conclusion: We revealed that hsa-miR-338-3p, down-regulated in GBM, may affect the biogenesis and rapid proliferation of glioma cells by regulating the level of OXSM, providing new insights into understanding the pathogenesis of GBM and developing strategies to improve GBM prognosis.

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“…MiR-NAs are a type of non-coding RNAs containing 21-25 nucleotides, and function as gene regulators by binding to target genes and inhibiting translation [8]. Many of these target genes are involved in fundamental biological processes such as cells differentiation, cells proliferation and cells migration [9][10][11][12]. The dysregulated miRNAs play a key role in tumorigenesis and tumor development and are related to poor prognosis in various carcinomas [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes

et al. 2020

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Background: Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908-3p in breast cancer. Methods: The expression of miR-1908-3p was detected in 50 pairs of breast cancer tissues and adjacent normal tissues, 60 breast cancer patient serum and 60 healthy volunteer serum. The functional roles of miR-1908-3p in breast cancer cells such as proliferation, migration and invasion were evaluated using CCK8, SRB, wound healing and transwell chambers. In addition, bioinformatics tools were used to identify potential targets of miR-1908-3p. Results: The results showed that the expression of miR-1908-3p were increased in breast cancer tissues and serum compared with normal breast tissues and serum of healthy volunteers respectively. Furthermore, the young breast cancer patients and HER2-positive patients had a higher level of tissues' miR-1908-3p than elder breast cancer patients and HER2-negative patients, respectively. The young breast cancer patients had a higher level of serum miR-1908-3p than elder breast cancer patients, ROC analysis suggested that miR-1908-3p had the potential as a promising serum diagnostic biomarker of breast cancer. Up-regulation of miR-1908-3p promoted the cells proliferation, migration and invasion while knockdown of miR-1908-3p inhibited these processes in breast cancer cell MCF-7 and MDA-MB-231. The potential target genes of miR-1908-3p in breast cancer included ID4, LTBP4, GPM6B, RGMA, EFCAB1, ALX4, OSR1 and PPARA. Higher expression of these eight genes correlated with a better prognosis for breast cancer patients. Conclusions: These results suggest that miR-1908-3p may exert its oncogenic functions via suppression of these eight genes in breast cancer.

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Retracted: MicroRNA‐150‐5p affects cell proliferation, apoptosis, and EMT by regulation of the BRAF^V600E mutation in papillary thyroid cancer cells

Cited by 31 publications

References 32 publications

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

<p>Reduced Expression of hsa-miR-338-3p Contributes to the Development of Glioma Cells by Targeting Mitochondrial 3-Oxoacyl-ACP Synthase (OXSM) in Glioblastoma (GBM)</p>

Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes

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