Retracted: miR‐96/HBP1/Wnt/β‐catenin regulatory circuitry promotes glioma growth

Abstract

Edited by Lukas HuberKeywords: Glioma miR-96 Wnt/b-catenin Proliferation HMG-box transcription factor 1 a b s t r a c tWe found that miR-96 is overexpressed in glioma, and its level inversely correlates with the survival of patients. The reduction in miR-96 abundance suppresses the proliferation and colony formation of glioma cells. The tumorigenicity of U-87 MG cells is reduced by miR-96 silencing. miR-96 contributes to the activation of Wnt/b-catenin pathway in glioma cells. HMG-box transcription factor 1 (HBP-1), a Wnt/b-catenin pathway inhibitor, is suppressed by miR-96. The reactivation of Wnt/bcatenin signaling causes an increase in the proliferation of glioma cells, and a decrease in miR-96 expression. On the other hand, HBP1 silencing promotes miR-96 expression. Collectively, miR-96 contributes to the progression of glioma by enhancing the activation of the Wnt/b-catenin pathway, and the miR-96/HBP1/Wnt/b-catenin regulatory circuitry promotes the proliferation of glioma cells.

Hbp1 regulates the timing of neuronal differentiation during cortical development by controlling cell cycle progression

“…Neural stem/progenitor cells are actively dividing in Hbp1-deficient mice It was previously reported that Hbp1 is involved in regulation of the Wnt signaling by inhibiting the function of TCF4-β-catenin complex via physical blockade of TCF/LEF-mediated DNA binding, and functions as a growth suppressor by repressing downstream genes of the Wnt signaling and cell cycle-related genes (Tevosian et al, 1997;Gartel et al, 1998;Sampson et al, 2001;Shih et al, 2001;Kim et al, 2006;Elfert et al, 2013;Yan et al, 2014). We therefore assessed cell proliferation activity in neural stem/progenitor cells in the developing cortex.…”

“…Among a variety of genes that were differentially expressed during the course of development, we hypothesized that high mobility group box transcription factor 1 (Hbp1) might be an important regulator of neurogenesis, given that it was upregulated during neurogenic stages around embryonic day 13.5 (E13.5) through embryonic day 15.5 (E15.5). Previous studies demonstrated that Hbp1 acts as a transcriptional repressor that functions as a cell cycle inhibitor by repressing downstream targets of the Wnt signaling and cell cyclerelated genes, cyclin D1 (Ccnd1), c-jun (Jun), N-myc (Mycn) and p21 (Cdkn1a) (Gartel et al, 1998;Sampson et al, 2001;Kim et al, 2006;Elfert et al, 2013;Yan et al, 2014).…”

The role of micro-RNA in the regulation of signal pathways in gliomas

“…MiR-96 и miR-155 гиперэкспрессированы при глиомах, и уровень miR-96 обратно коррелирует с выживаемостью пациентов [48,49].…”

miR-155 contributes to the progression of glioma by enhancing Wnt/β-catenin pathway

“…The procedures of the isolation and culturing of primary astrocytes have been described previously [7], which have been approved by the Ethical Review Board in the Affiliated Hospital of Medical College of Qingdao University (Qingdao, China).…”

“…To study the role of miR-155 in the tumorigenesis of glioma cell lines, two different mice models were used. The details of the establishment of glioma xenografts were available in previous papers [7]. Briefly, U-87 MG glioma xenograft was established by injecting 5×10 6 cells into the flanks of 4-5-week-old male BALB/c nude mice.…”