<i>RbohB</i>, a <i>Phaseolus vulgaris </i><scp>NADPH</scp> oxidase gene, enhances symbiosome number, bacteroid size, and nitrogen fixation in nodules and impairs mycorrhizal colonization

Arthikala, Manoj‐Kumar; Sánchez‐López, Rosana; Nava, Noreide; Santana, Olivia; Cárdenas, Luis; Quinto, Carmen

doi:10.1111/nph.12714

Cited by 88 publications

(87 citation statements)

References 70 publications

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“…Larger and heavier nodules are considered more important than a high number of them, since many small nodules might not reflect a good N 2 fixation ability . Moreover, the occupation of nodules by active bacteroids is also very important for the symbiosis (Arthikala et al, 2014). The number of nodules and the nodule dry weight are phenotypic characteristics considered as representatives for identifying quantitative trait loci (QTL) for biological N fixation (Santos et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Variability of nodulation traits in Andean and Mesoamerican common bean gene pools

Knupp

Ferreira

Araújo

2017

Pesq. agropec. bras.

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-The objective of this work was to evaluate the genotypic diversity for nodulation in common bean (Phaseolus vulgaris) genotypes of Andean and Mesoamerican gene pools present in the core collections of Embrapa, in Brazil. Evaluations were carried out in two stages: the first one with 879 genotypes, taking the cultivar 'Ouro Negro' as reference; and the second one with 116 genotypes with greater nodulation, to identify those showing stability in nodulation in the two stages. Rhizobium strains were inoculated in pre-germinated common bean seed. Plants received weekly a nutrient solution without N, and nodulation was evaluated 35 days after planting. The percentage of genotypes that exceeded the reference cultivar was 22% for number of nodules, 46% for nodule dry weight, and 33% for one nodule dry weight. The combined analysis of data from the two evaluation phases showed wide differences among genotypes, and significant interactions between genotypes and stages. By the orthogonal contrasts analysis, the Mesoamerican genotypes showed a greater nodulation than the Andean ones. In both stages, twenty-six more stable genotypes were identified, most of them belonging to the Mesoamerican gene pool. There is a large variability in nodulation traits among the common bean genotypes present in the core germplasm collections of Embrapa, which suggests that there are genotypes with improved nodulation.Index terms: Phaseolus vulgaris, biological nitrogen fixation, diversity, germplasm bank. Variabilidade das características de nodulação em feijoeirocomum dos conjuntos gênicos andino e mesoamericanoResumo -O objetivo deste trabalho foi avaliar a diversidade genotípica quanto à nodulação em genótipos de feijoeiro-comum (Phaseolus vulgaris) pertencentes aos conjuntos gênicos andino e mesoamericano presentes nas coleções nucleares da Embrapa, no Brasil. As avaliações foram realizadas em dois estágios: o primeiro com 879 genótipos, tendo-se a cultivar 'Ouro Negro' como referência; e o segundo, com 116 genótipos de maior nodulação, para identificar aqueles com estabilidade de nodulação nos dois estágios. Estirpes de Rhizobium foram inoculadas em sementes de feijão pré-germinadas. As plantas receberam, semanalmente, uma solução nutritiva isenta de N, e a nodulação foi avaliada 35 dias após o plantio. O percentual de genótipos que superaram a cultivar-referência foi de: 22%, quanto ao número de nódulos; 46%, quanto à massa de matéria seca de nódulos; e 33%, quanto à massa de matéria seca de um nódulo. A análise conjunta dos dados das duas fases de avaliação identificou amplas diferenças entre genótipos e interações significativas entre genótipos e estágios. Na análise de contrastes ortogonais, os genótipos mesoamericanos apresentaram maior nodulação do que os andinos. Foram identificados 26 genótipos mais estáveis nos dois estágios, a maioria deles pertencente ao conjunto gênico mesoamericano. Há uma ampla variabilidade em caracteres relacionados à nodulação entre os genótipos de feijoeiro presentes nas coleções nucleares da ...

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Section: Resultsmentioning

confidence: 99%

Variability of nodulation traits in Andean and Mesoamerican common bean gene pools

Knupp

Ferreira

Araújo

2017

Pesq. agropec. bras.

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“…concentrations could change the outcome of the symbiosis by being perceived as a signaling molecule (Kiirika et al 2012;Arthikala et al 2014;Belmondo et al 2016). …”

Section: Hydrogen Peroxide Treatment: Identification Of the Main Genementioning

confidence: 99%

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

et al. 2017

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Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to H2O2 in Gigaspora margarita, an AM fungus containing the endobacterial symbiont Candidatus Glomeribacter gigasporarum (CaGg). Previous studies revealed that G. margarita with its endobacterium produces more ATP and has higher respiratory activity compared to a cured line that lacks the endobacterium. This higher bioenergetic potential leads to higher production of ROS, and to a higher ROS-detoxifying capacity, suggesting a direct or indirect role of the endobacterium in modulating fungal anti-oxidant responses. To test the hypothesis that the fungal-endobacterial symbiosis may enhance the fitness of the AM fungus in the presence of oxidative stress, we treated the fungus with a sublethal concentration of H2O2 and performed RNA-seq analysis. Our results demonstrate that: i) irrespective of the endobacterium presence, G. margarita faces oxidative stress by activating multiple metabolic processes (methionine oxidation, sulfur uptake, the pentose phosphate pathway, activation of ROS-scavenger genes), ii) in the presence of its endobacterium, G. margarita upregulates some metabolic pathways, like chromatin status modifications and iron metabolism; iii) contrary to our hypothesis, the cured line responds to H2O2 by activating the transcription of specific ROS scavengers. We confirmed the RNA-seq findings by measuring the glutathione and ascorbate content, which was the same in both lines after H2O2 treatment. We conclude that both fungal lines

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“…In the present-day, vast efforts have been made to genetically transform plants to improve their tolerance. Although some increased tolerance to pathogens has been reported in transgenic peppers [34,35] other approaches to achieve resistance must be currently considered as genetic engineering means in plants has been poorly accepted by the public [36].…”

Section: Introductionmentioning

confidence: 99%

Pepper Crop under Climate Change: Grafting as an Environmental Friendly Strategy

Penella¹,

Calatayud²

2018

Climate Resilient Agriculture - Strategies and Perspectives

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Pepper is an extremely important vegetable worldwide in socio-economic terms. However, persistent land use, monoculture, and intensified production processes have led to soil diseases. This, along with abiotic stress, and mainly salinity of soil and waters, water stress, and suboptimal temperatures, can lead to physiological disorders emerging in peppers, e.g., cracking and Blossom end rot, which induce plant senescence, and lower not only in yields, but also in product quality. Salinity and water shortage are the two main environmental problems that crops face in the Mediterranean Region. One way of overcoming stresses from an ecological or integrated crop management viewpoint is to use grafted plants as an adaptation strategy. Initially, grafting technology has expanded in Solanaceae and Cucurbitacea species to overcome biotic stress. Nowadays, grafts are being used as several approaches to cushion the impact of climate change on agricultural systems. Furthermore, grafts allow desirable varieties by organoleptic or productivity traits, but they are sensitive to abiotic stress and can be grown under abiotic stress. As far as we know, very few studies on grafted pepper plants under abiotic stress are available.

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RbohB, a Phaseolus vulgaris NADPH oxidase gene, enhances symbiosome number, bacteroid size, and nitrogen fixation in nodules and impairs mycorrhizal colonization

Cited by 88 publications

References 70 publications

Variability of nodulation traits in Andean and Mesoamerican common bean gene pools

Variability of nodulation traits in Andean and Mesoamerican common bean gene pools

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

Pepper Crop under Climate Change: Grafting as an Environmental Friendly Strategy

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