Rabdosia japonica var. glaucocalyx Flavonoids Fraction Attenuates Lipopolysaccharide‐Induced Acute Lung Injury in Mice

Abstract

Rabdosia japonica var. glaucocalyx (Maxim.) Hara, belonging to the Labiatae family, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers. Aim of the Study. To investigate therapeutic effects and possible mechanism of the flavonoids fraction of Rabdosia japonica var. glaucocalyx (Maxim.) Hara (RJFs) in acute lung injury (ALI) mice induced by lipopolysaccharide (LPS). Materials and Methods. Mice were orally administrated with RJFs (6.4, 12.8, and 25.6 mg/kg) per day for 7 days, consecutively, before LPS challenge. Lung specimens and the bronchoalveolar lavage fluid (BALF) were isolated for histopathological examinations and biochemical analysis. The level of complement 3 (C3) in serum was quantified by a sandwich ELISA kit. Results. RJFs significantly attenuated LPS-induced ALI via reducing productions of the level of inflammatory mediators (TNF-α, IL-6, and IL-1β), and significantly reduced complement deposition with decreasing the level of C3 in serum, which was exhibited together with the lowered myeloperoxidase (MPO) activity and nitric oxide (NO) and protein concentration in BALF. Conclusions. RJFs significantly attenuate LPS-induced ALI via reducing productions of proinflammatory mediators, decreasing the level of complement, and reducing radicals.

Eupatorium lindleyanum DC. sesquiterpenes fraction attenuates lipopolysaccharide-induced acute lung injury in mice

“…In our investigation (Chu et al, 2014), we selected time points which data is stable and significantly different from the control group as the monitoring point in time. Therefore, 6 h after LPS challenge we measured NO and total protein in BALF, C3 and C3c in serum, obtained the lung W/D ratio.…”

“…The experimental protocols shown in this study were approved by the Animal Ethical Committee of School of Pharmacy at Soochow University. (Xie et al, 2012;Chi et al, 2013;Chu et al, 2014) EUP-SQT was ground and suspended in distilled water containing 0.5% sodium carboxymethyl cellulose (CMC-Na) for administration to mice.…”

Eupatorium lindleyanum DC. flavonoids fraction attenuates lipopolysaccharide-induced acute lung injury in mice

“…The experimental protocols shown in this study were approved by the Animal Ethical Committee of School of Pharmacy at Soochow University. [12,[18][19][20][21] EUP-FLA was ground and suspended in distilled water containing 0.5% sodium carboxymethyl cellulose (CMC-Na) for administration to mice.…”

Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract

“…The plant Rabdosia japonica is used in folk medicine for the treatments of abdominal swelling and pain, gastric carcinoma, hepatocarcinoma and esophageal carcinoma (Cao & Chen, ; Gao, Yu, & Liang, ), and has been increasingly studied for its anti‐melanogenesis, antioxidant, anti‐inflammatory, antibacterial and cytotoxicity activities (Chu et al, ; Liu et al, ; Masuoka, Isobe, & Kubo, ; Satooka, Isobe, Nitoda, & Kubo, ; Xu, Chu, Xia, Zhang, & Chen, ; Zhao, Xuan, Yuan, Gan, & Xiao, ). Weifuchun tablet (a preparation containing the raw R. japonica material) is employed in Chinese medicine for the treatment of early gastric cancer and chronic atrophic gastritis (Zhang, Tian, & Chen, ).…”