Pterocarpus santalinus L. Regulated Ultraviolet B Irradiation‐induced Procollagen Reduction and Matrix Metalloproteinases Expression Through Activation of TGF‐β/Smad and Inhibition of the MAPK/AP‐1 Pathway in Normal Human Dermal Fibroblasts

Abstract

Ultraviolet light-induced reactive oxygen species (ROS) damage human skin and prematurely cause aging. A growing body of research is focusing on considering plants and plant-derived compounds as antiphotoaging therapeutic material. Pterocarpus santalinus L., as an Indian traditional medicine, possesses antidiabetic, anti-inflammatory and antioxidative effects. Here, we studied the antiphotoaging effects of ethanolic extract of P. santalinus L. heartwood (EPS) on ultraviolet radiation B (UVB)-irradiated normal human dermal fibroblasts (NHDFs). Results showed that EPS significantly inhibited the upregulation of matrix metalloproteinases and IL-6 caused by UVB irradiation, and suppressed UVB-induced phosphorylation of extracellular signal-regulated kinase, Jun N-terminal kinase and p38, as well as the activation of AP-1 transcription factors. Further study indicated that UVB-induced production of MMP-1 and IL-6 could be inhibited by PD 98059 (an ERK inhibitor) and SP600125 (A JNK inhibitor), implied that EPS inhibited UVB-induced MMP-1 and IL-6 secretion by inactivating MAPK signaling pathway. In addition, EPS possessed an excellent antioxidant activity, which could increase cytoprotective antioxidants such as HO-1, NQ-O1 expression by facilitating the nuclear accumulation of Nrf2. Treatment of NHDFs with EPS also recovered UVB-induced procollagen type I reduction by activating TGF-β/Smad pathway. These findings demonstrated that EPS had a potential effect against UVB-induced skin photoaging.

A Peptide YGDEY from Tilapia Gelatin Hydrolysates Inhibits UVB‐mediated Skin Photoaging by Regulating MMP‐1 and MMP‐9 Expression in HaCaT Cells

“…Among them, JNK affects the synthesis of pro‐collagen I. It has been shown that quercetin inhibits UV‐induced pro‐collagen I degradation by reducing JNK activity . After UVB‐induced, phosphorylation of p38, JNK and ERK activate downstream‐related pathway NF‐ κ B .…”

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

“…The skin aging system is composed of several processes including those of hereditary factors and environmental factors that are normally associated with sun exposure. Repeated exposure to UVB irradiation can induce generation of reactive oxygen species (ROS) to cause skin aging and pigmentation [2,3]. Moreover, the increase in ROS generated by UV irradiation not only induces cell death, but also increases expression levels of matrix metalloproteinases (MMPs) [4][5][6].…”

Synergistic Promotion on Tyrosinase Inhibition by Antioxidants

“…Nrf2, an essential transcription factor for controlling oxidative stress [ 33 ], is expressed in human skin tissues, such as human keratinocytes [ 34 , 35 ], melanocyte [ 4 ], and human dermal fibroblasts [ 36 ]. Cytoprotective antioxidants can be increased by antioxidant compounds through the nuclear accumulation of Nrf2 [ 36 , 37 ]. Some necessary connections seemingly exist between these two protective mechanisms of skin tissues because they both participate in ROS removal.…”