<i>PSK1</i>regulates expression of<i>SOD1</i>involved in oxidative stress tolerance in yeast

Huang, Meixian; Xu, Qinghong; Mitsui, Kazuhiro; Xu, Zhaojun

doi:10.1111/1574-6968.12329

Cited by 12 publications

(18 citation statements)

References 23 publications

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“…The lack of an effective antioxidant defense system leads to an accumulation of hydrogen peroxide and/or other reactive oxygen species (ROS). The enzymatic detoxification of ROS is partially dependent on the activation of antioxidant genes such as CAT1 and SOD1 (Deveau et al, 2010;Huang et al, 2014;Jung and Kim, 2014). In the present study, the expression of two antioxidant genes (Figure 3) was elevated in MT and ST cells, which may have contributed to an increased ability to scavenge intracellular ROS (Figure 2) in yeast cells, and thus a higher level of viability (Figure 1).…”

Section: Discussionmentioning

confidence: 46%

Pretreatment of the Antagonistic Yeast, Debaryomyces hansenii, With Mannitol and Sorbitol Improves Stress Tolerance and Biocontrol Efficacy

2020

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The effect of exogenous mannitol and sorbitol on the viability of the antagonist yeast, Debaryomyces hansenii, when exposed to oxidative and high-temperature stress was determined. Results indicated that both the 0.1 M mannitol (MT) and 0.1 M sorbitol (ST) treatments improved the tolerance of D. hansenii to subsequent oxidative and hightemperature stress. MT or ST cells had a significantly higher level of cell survival, elevated the gene expression of catalase 1 (CAT1) and copper-zinc superoxide dismutase (SOD1), as well as the corresponding enzyme activity. Treated cells also exhibited a lower accumulation of intracellular reactive oxygen species (ROS), and a higher content of intracellular mannitol and sorbitol relative to non-treated, control yeast cells, when exposed to a subsequent oxidative (30 mM H 2 O 2 ) or heat (40.5 • C) stress for 30 min. Additionally, MT and ST yeast exhibited a higher growth rate in kiwifruit wounds, and a greater ability to inhibit postharvest blue mold (Penicillium expansum) and gray mold (Botrytis cinerea) infections. The present study indicates that increased antioxidant response induced by mannitol and sorbitol in D. hansenii can enhance stress tolerance and biocontrol performance.

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Section: Discussionmentioning

confidence: 46%

Pretreatment of the Antagonistic Yeast, Debaryomyces hansenii, With Mannitol and Sorbitol Improves Stress Tolerance and Biocontrol Efficacy

2020

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“…The PSK1 mRNA was induced in the respiratory phase, and its level was about four folds higher than that of the respire‐fermentative phases. To confirm the importance of PSK1 in stabilization of EMO, PSK1 was deleted and replaced with Kan r gene by using the PCR‐Knockout technology (Huang et al, ). The EMO in wild‐type yeast cells in aerobic chemostat cultures over a 4‐hr period consists of respiratory and respire‐fermentative phases with low and high dissolved oxygen concentration, respectively, according to oxygen demand (Wang et al, ; Xu & Tsurugi, ).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, nonfermentative carbon sources activate PSK1, whereas cell integrity stress activates PSK2 (Grose et al, ). We also found that the two yeast PAS kinases respond differently to stress stimulation; deletion of PSK1 increased the sensitivity of yeast cells to oxidative stress and partially inhibited cell growth, whereas the growth of the PSK2 ‐deleted mutant was similar to that of the wild type (Huang, Xu, Mitsui, & Xu, ). PSK2 kinase is also important for the uptake of glucose and regulation of storage carbohydrate synthesis and hence, the maintenance of an unperturbed continuously oscillating state (Ouyang, Xu, Mitsui, Motizuki, & Xu, ), whereas the role of PSK1 in the regulation of EMO remains unclear.…”

Section: Introductionmentioning

confidence: 86%

“…The PSK1 mutant (PSK1Δ) was deleted in S288C as described previously (Huang et al, 2014). Briefly, disruption cassette loxP-kanMX-loxP was used for deletion of PSK1, and a DNA fragment carrying replacement of PSK1 with Kan r gene was generated by polymerase chain reaction (PCR) using plasmid pUG6 as template (Gueldener, Heinisch, Koehler, Voss, & Hegemann, 2002;Ouyang, Xu, Mitsui, Motizuki, & Xu, 2009).…”

Section: Construction Of Psk1δmentioning

confidence: 99%

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PSK1 coordinates glucose metabolism and utilization and regulates energy‐metabolism oscillation in Saccharomyces cerevisiae

Huang

Ouyang

et al. 2020

Yeast

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Energy‐metabolism oscillations (EMO) are ultradian biological rhythms observed in in aerobic chemostat cultures of Saccharomyces cerevisiae. EMO regulates energy metabolism such as glucose, carbohydrate storage, O2 uptake, and CO2 production. PSK1 is a nutrient responsive protein kinase involved in regulation of glucose metabolism, sensory response to light, oxygen, and redox state. The aim of this investigation was to assess the function of PSK1 in regulation of EMO. The mRNA levels of PSK1 fluctuated in concert with EMO, and deletion of PSK1 resulted in unstable EMO with disappearance of the fluctuations and reduced amplitude, compared with the wild type. Furthermore, the mutant PSK1Δ showed downregulation of the synthesis and breakdown of glycogen with resultant decrease in glucose concentrations. The redox state represented by NADH also decreased in PSK1Δ compared with the wild type. These data suggest that PSK1 plays an important role in the regulation of energy metabolism and stabilizes ultradian biological rhythms. These results enhance our understanding of the mechanisms of biorhythms in the budding yeast.

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“…For example, PAS domains are present in the serine/threonine protein kinases PSK1 and PSK2 in yeast [9]. In yeast, PSK1 regulates superoxide dismutase-1 (SOD1) to protect against oxidative stress, and PSK2 acts as a nutrient-sensing kinase [10]. In the filamentous fungus Neurospora crassa, DCC-1, a putative HK, is involved in the regulation of conidiation, perithecial development, and carotenogenesis [11].…”

Section: Introductionmentioning

confidence: 99%

A PAS-Containing Histidine Kinase is Required for Conidiation, Appressorium Formation, and Disease Development in the Rice Blast Fungus,Magnaporthe oryzae

et al. 2019

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Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most important diseases in rice production. PAS (period circadian protein, aryl hydrocarbon receptor nuclear translocator protein, single-minded protein) domains are known to be involved in signal transduction pathways, but their functional roles have not been well studied in fungi. In this study, targeted gene deletion was carried out to investigate the functional roles of the PAS-containing gene MoPAS1 (MGG_02665) in M. oryzae. The deletion mutant DMopas1 exhibited easily wettable mycelia, reduced conidiation, and defects in appressorium formation and disease development compared to the wild type and complemented transformant. Exogenous cAMP restored appressorium formation in DMopas1, but the shape of the restored appressorium was irregular, indicating that MoPAS1 is involved in sensing the hydrophobic surface. To examine the expression and localization of MoPAS1 in M. oryzae during appressorium development and plant infection, we constructed a MoPAS1:GFP fusion construct. MoPAS1:GFP was observed in conidia and germ tubes at 0 and 2 h post-infection (hpi) on hydrophobic cover slips. By 8 hpi, most of the GFP signal was observed in the appressoria. During invasive growth in host cells, MoPAS1:GFP was found to be fully expressed in not only the appressoria but also invasive hyphae, suggesting that MoPAS may contribute to disease development in host cells. These results expand our knowledge of the roles of PAS-containing regulatory genes in the plant-pathogenic fungus M. oryzae.

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PSK1regulates expression ofSOD1involved in oxidative stress tolerance in yeast

Cited by 12 publications

References 23 publications

Pretreatment of the Antagonistic Yeast, Debaryomyces hansenii, With Mannitol and Sorbitol Improves Stress Tolerance and Biocontrol Efficacy

Pretreatment of the Antagonistic Yeast, Debaryomyces hansenii, With Mannitol and Sorbitol Improves Stress Tolerance and Biocontrol Efficacy

PSK1 coordinates glucose metabolism and utilization and regulates energy‐metabolism oscillation in Saccharomyces cerevisiae

A PAS-Containing Histidine Kinase is Required for Conidiation, Appressorium Formation, and Disease Development in the Rice Blast Fungus,Magnaporthe oryzae

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