Pseudomonas aeruginosabiofilm formation and slime excretion on antibiotic-loaded bone cement

Abstract

In the clinical situation, our findings help to explain the frequent re-implantation failure of joint replacements infected with P. aeruginosa when the procedure has been performed using antibiotic-loaded bone cement.

The Influence of Maggot Excretions on PAO1 Biofilm Formation on Different Biomaterials

“…We believe this work may be relevant to patient care, because biofilms make treatment of infected orthopaedic implants and prostheses more difficult [6,19,32]. For the future, the influence of sterile ES on BF by different bacterial species on orthopaedic implants is our current topic of research.…”

“…BF on orthopaedic biomaterials causes severe infections that are very difficult to treat and often lead to invalidating consequences for patients [6,19,32]. Therefore, it is important to investigate new treatment possibilities for these kind of infections.…”

“…Once infected, the implant often must be removed [18,32]. Temporary implantation of antibiotic beads is sometimes necessary [19].…”

Reinfected Revised TKA Resolves With an Aggressive Protocol and Antibiotic Infusion

“…A low concentration of antibiotics in the synovial fluid and on the surface of the cement spacer fosters the development of resistant bacteria [21]. Small colony variants require sustained, high antibiotic concentrations and longer exposure to high antibiotic levels than can be obtained with intravenous antibiotics and antibioticloaded cement spacers [21,24].…”

“…Antibiotic release is minimal after 5 days [11], but the levels remain detectable for as long as 340 days postoperatively [15]. These levels may be too low to be bactericidal in resistant bacteria or gram-negative organisms, even in the first few days, and are likely to be ineffective as time passes [21]. A low concentration of antibiotics in the synovial fluid and on the surface of the cement spacer fosters the development of resistant bacteria [21].…”