Potato virus Y infection hinders potato defence response and renders plants more vulnerable to Colorado potato beetle attack

Abstract

In the field, plants are challenged by more than one biotic stressor at the same time. In this study, the molecular interactions between potato (Solanum tuberosum L.), Colorado potato beetle (Leptinotarsa decemlineata Say; CPB) and Potato virus Y NTN (PVY NTN ) were investigated through analyses of gene expression in the potato leaves and the gut of the CPB larvae, and of the release of potato volatile compounds. CPB larval growth was enhanced when reared on secondary PVY NTN -infected plants, which was linked to decreased accumulation of transcripts associated with the antinutritional properties of potato. In PVY NTN -infected plants, ethylene signalling pathway induction and induction of auxin response transcription factors were attenuated, while no differences were observed in jasmonic acid (JA) signalling pathway. Similarly to rearing on virusinfected plants, CPB larvae gained more weight when reared on plants silenced in JA receptor gene (coi1). Although herbivore-induced defence mechanism is regulated predominantly by JA, response in coi1-silenced plants only partially corresponded to the one observed in PVY NTN -infected plants, confirming the role of other plant hormones in modulating this response. The release of b-barbatene and benzyl alcohol was different in healthy and PVY NTN -infected plants before CPB larvae infestation, implicating the importance of PVY NTN infection in plant communication with its environment. This was reflected in gene expression profiles of neighbouring plants showing different degree of defence response. This study thus contributes to our understanding of plant responses in agro-ecosystems.

RNA‐seq reveals regional differences in transcriptome response to heat stress in the marine snail Chlorostoma funebralis

“…; Petek et al . ; Westram et al . ) and both our methodology itself and the reporting of our methods in the manuscript reaches, and in some instances exceeds, the standards set by these recent publications.…”

Effect of starvation on expression of troponin complex genes and ER stress associated genes in skeletal muscles

“…It is known that starvation influences expression levels of several genes such as allatotropin in the oriental armyworm, Mythimna separata, different mitochondrial genes in the mosquito, Aedes aegypti, and tissue-specific genes in the wood ant, Formica exsecta [15][16][17]. Starved insects are more susceptible to pathogens due to their compromised immune systems (e.g., Colorado potato beetle, Leptinotarsa decemlineata) [18]. During starvation, differential gene expression in skeletal muscles has been reported in vertebrates, with ER-stress associated gene as a potential mediator of anabolic resistance [19].…”

Achievements and prospects of applying high-throughput sequencing techniques to potato genetics and breeding

“…В этом разделе суммированы данные 1 583 экспериментов, посвященных анализу транскриптома картофеля. Результаты транс криптомных экспериментов в БД GEO представлены в 30 публикациях, в число которых входят исследования по изменению транскриптома картофеля в ответ на за ражение вирусами Petek et al, 2014;Stare et al, 2015), на воздействие факторами абиотического стресса (Gálvez et al, 2016), различия в уровне экспрессии генов miRNA в зависимости от стадии развития (Lakhotia et al, 2014). Первичные данные секвенирования транс криптомов в экспериментах RNAseq представлены в базе данных NCBI SRA (Sequence Read Archive), основном мировом архиве результатов высокопроизводительного секвенирования нуклеотидных последовательностей.…”