<i>Porphyromonas gingivalis</i> biofilm formation in different titanium surfaces, an <i>in vitro</i> study

Giulio, Mara Di; Traini, Tonino; Sinjari, Bruna; Nostro, Antonia; Caputi, Sergio; Cellini, Luigina

doi:10.1111/clr.12659

Cited by 49 publications

(55 citation statements)

References 47 publications

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“…It seems that the formed grooves HSM-MQL surface treatment (figure 2) provide a good environment for the growth of bacteria. This observation is in line with Giulio et al's report [46]. From figure 7(a), it could be observed that the water permeation through the coating can be high due to the coating's increased hydrophilicity.…”

Section: Microbiological Studiessupporting

confidence: 91%

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

Koopaie

Bordbar‐Khiabani

Kolahdooz

et al. 2020

Mater. Res. Express

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Topography and surface chemistry can significantly affect biofilm formation on dental implants. Recently, the γ-TiAl alloy was considered as the most reliable candidates for the preparation of dental implants because of its excellent mechanical strength, chemical stability and biocompatibility. The emphasis of this study lies in the effects of high-speed milling assisted the minimum quantity of lubrication (HSM-MQL), micro-current wire electrical discharge machining (mWEDM), Er,Cr:YSGG laser and sandblasting/largegrit/acid-etching (SLA) treatments on surface morphology, topography, chemical composition, wettability and biofilm-associated infections on the surface of each group. The surface-treated samples were analyzed using a scanning electron microscope (SEM), SEM surface reconstruction, energy dispersive x-ray spectroscopy (EDS) and water contact angle measuring system. SEM and topography images of mWEDM and laser-treated surfaces showed more irregular surfaces compared to SLA and HSM-MQL surfaces. Results showed that mWEDM and laser-treated surfaces revealed hydrophobic behavior. A significant decrease of biofilm formation was observed on mWEDM treated surface due to the hydrophobicity and existence of the copper element in the recast layer chemical composition. Moreover, EDS confirmed that the zirconium, silicon, and fluorine elements were decorated onto the SLA treated γ-TiAl surface that can have a direct effect on the anti-bacterial activity.

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Section: Microbiological Studiessupporting

confidence: 91%

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

Koopaie

Bordbar‐Khiabani

Kolahdooz

et al. 2020

Mater. Res. Express

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“…Furthermore, specific microsurface topography with increased relative roughness and hydrophobicity has been developed to enhance the implant-tissue interactions. These complex surface topographies, which clearly enhance implant osseointegration, may also facilitate the development of complex biofilms and impair their cleanability (Ferreira Ribeiro et al, 2016;Di Giulio et al, 2016;Song, Koo, & Ren, 2015;Violant, Galofre, Nart, & Teles, 2014;Xing, Lyngstadaas, Ellingsen, Taxt-Lamolle, & Haugen, 2015). Previous in vitro research on implant surfaces has shown that roughness, surface free energy, wettability, and degree of sterilization may affect biofilm formation, bacterial three-dimensional distribution, and antimicrobial treatment efficacy (Al-Ahmad et al, 2010;Di Giulio et al, 2016;Lin, Liu, Wismeijer, Crielaard, & Deng, 2013;Schmidlin et al, 2013;Song et al, 2015;Yeo, Kim, Lim, & Han, 2012).…”

mentioning

confidence: 99%

“…These complex surface topographies, which clearly enhance implant osseointegration, may also facilitate the development of complex biofilms and impair their cleanability (Ferreira Ribeiro et al, 2016;Di Giulio et al, 2016;Song, Koo, & Ren, 2015;Violant, Galofre, Nart, & Teles, 2014;Xing, Lyngstadaas, Ellingsen, Taxt-Lamolle, & Haugen, 2015). Previous in vitro research on implant surfaces has shown that roughness, surface free energy, wettability, and degree of sterilization may affect biofilm formation, bacterial three-dimensional distribution, and antimicrobial treatment efficacy (Al-Ahmad et al, 2010;Di Giulio et al, 2016;Lin, Liu, Wismeijer, Crielaard, & Deng, 2013;Schmidlin et al, 2013;Song et al, 2015;Yeo, Kim, Lim, & Han, 2012). These studies have used specimens, such as disks or slabs containing the studied surface (Aguayo, Donos, Spratt, & Bozec, 2015;de Avila et al, 2015;Di Giulio et al, 2016;Papavasileiou, Behr, Gosau, Gerlach, & Buergers, 2015;Pita et al, 2015;Ready et al, 2015), but these specimens lack the macro-structural and topographic characteristics of the dental implants used clinical practice.…”

mentioning

confidence: 99%

“…Previous in vitro research on implant surfaces has shown that roughness, surface free energy, wettability, and degree of sterilization may affect biofilm formation, bacterial three-dimensional distribution, and antimicrobial treatment efficacy (Al-Ahmad et al, 2010;Di Giulio et al, 2016;Lin, Liu, Wismeijer, Crielaard, & Deng, 2013;Schmidlin et al, 2013;Song et al, 2015;Yeo, Kim, Lim, & Han, 2012). These studies have used specimens, such as disks or slabs containing the studied surface (Aguayo, Donos, Spratt, & Bozec, 2015;de Avila et al, 2015;Di Giulio et al, 2016;Papavasileiou, Behr, Gosau, Gerlach, & Buergers, 2015;Pita et al, 2015;Ready et al, 2015), but these specimens lack the macro-structural and topographic characteristics of the dental implants used clinical practice. Furthermore, many of these in vitro investigations assessing bacterial adhesion and colonization on implant surfaces have used simple biofilm models, consisting of one or, maximum, two bacterial species, and/ or have used short-term evaluations (24 hr or less), thus, lacking the ability to properly study the dynamics of the biofilm maturation and its potential pathogenicity (Pereira et al, 2015;Schmidt et al, 2017;Sridhar et al, 2016).…”

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confidence: 99%

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Topographic characterization of multispecies biofilms growing on dental implant surfaces: An in vitro model

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

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Objectives To describe the early biofilm formation over whole dental implants with its micro‐ and macrostructure, using an in vitro multispecies biofilm model. Material and methods Six bacterial strains (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) were used to develop in vitro biofilms over whole titanium implants (growth times 12, 24, 48, 72, 96, and 120 hr). The morphology of biofilms was studied by confocal laser scanning microscopy and scanning electron microscopy, and the bacterial dynamics through quantitative polymerase chain reaction. As primary outcome variable, numbers of each species [colony‐forming units per milliliter (CFU/ml)] at different incubation times were compared using the one‐way analysis of variance and post hoc testing with Bonferroni's correction. Furthermore, implants were fixed in methacrylate stents to reproduce the clinical situation and biofilms were developed and analyzed by scanning electron microscopy. Results Bacteria colonized implants in a short period of time. Biofilms reached a mature state at 96 hr, exhibiting different ratios of live/dead cells depending on their location, being the peaks of the threads the areas harboring more live bacteria. The densities of each bacteria fluctuated in time, reaching its maximum at 96 hr. Even though the coefficients of variation were high, percentages were similar to those published previously using implant surface specimens, rather than whole implants. Conclusion Dental implants can be colonized by different bacterial species, developing into a mature and well‐structured biofilm, which depending on the location may exhibit different degree of bacterial vitality.

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“…In the 2015, Di Giulio et al showed that the modification of the titanium surfaces can influence the colonization and biofilm formation of Porphyromonas gingivalis ; the surface treatment overcomes the differences in the material composition [16]. In addition, the difference in titanium surface roughness was associated with variations in the antifungal resistance of the candida biofilm [17].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Analysis of Retrieved Dental Implants after Different Peri-Implantitis Treatments

Geremias

Montero

Magini

et al. 2017

Case Reports in Dentistry

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The aim of the current study was to analyse the planktonic growth of Streptococcus mutans on the surfaces of three implants retrieved after three different peri-implantitis treatments. Three implants from a male patient with high levels of bone loss were treated by mechanical debridement, chemical decontamination, and implantoplasty. After 4 months of follow-up, the implants were removed. The growth and biofilm formation were measured by spectrophotometry (OD630 nm) and scanning electron microscopy (SEM), after 48 hours of incubation. Results showed an average of Streptococcus mutans planktonic growth over the implants of 0.21 nm (mechanical debridement), 0.16 nm (chemical decontamination), and 0.15 nm (implantoplasty). Data were analysed by ANOVA and Tukey's test (p < 0.05 for chemical decontamination and implantoplasty). Implantoplasty and chemical decontamination showed the lowest levels of planktonic growth, indicating a possible influence of the modification procedures on the titanium surface on the initial biofilm attachment.

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Porphyromonas gingivalis biofilm formation in different titanium surfaces, an in vitro study

Abstract: Within the limits of the present study, the results showed that G4-L appears to be significantly efficient in the reduction of the P. gingivalis biofilm formation.

Cited by 49 publications

References 47 publications

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

Advanced surface treatment techniques counteract biofilm-associated infections on dental implants

Topographic characterization of multispecies biofilms growing on dental implant surfaces: An in vitro model

Biofilm Analysis of Retrieved Dental Implants after Different Peri-Implantitis Treatments

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