Pleurotus albidus Modulates Mitochondrial Metabolism Disrupted by Hyperglycaemia in EA.hy926 Endothelial Cells

Abstract

Hyperglycaemia exacerbates the production of reactive oxygen species (ROS), contributing to the multiple complications associated with diabetes. Mitochondrial dysfunction is also known to be associated with diabetes. Therefore, the aim of this work was to study the effect of Pleurotus albidus extract on the mitochondrial dysfunction induced by hyperglycaemia in EA.hy926 endothelial cells. The results showed that P. albidus treatment prevented the increase in the activity of complex I of the electron transport chain and minimized the ROS production induced by hyperglycaemia. In addition, the extract minimized oxidative damage to lipids and proteins, caused an imbalance in the antioxidant enzyme activities of superoxide dismutase and catalase, and decreased the nitric oxide levels induced by hyperglycaemia. These data contribute to our understanding of the mitochondrial disorder induced by hyperglycaemia as well as establishing the conditions required to minimize these alterations.

[Retracted] Natural Antioxidants Improve the Vulnerability of Cardiomyocytes and Vascular Endothelial Cells under Stress Conditions: A Focus on Mitochondrial Quality Control

“…Resveratrol inhibits high glucose-induced mtROS production in human coronary artery endothelial cells via the intracellular target protein deacetylase silent information regulator (SIRT)2/SIRT1 [ 153 ]. In endothelial cells, SIRT1 overexpression attenuates mtROS production and overexpression of RSV and SIRT1 significantly reduces the level of H 2 O 2 and increased Mn-SOD and GSH expression in a concentration-dependent manner.…”

“…It inhibits the increase in electron transport chain complex I activity and decrease in ROS production induced by hyperglycaemia. It suppresses the oxidative damage of lipids and proteins, regulates imbalanced SOD and CAT activities, reduces the level of nitric oxide, restores mitochondrial function, and improves the vulnerability of endothelial cells to hyperglycaemia [ 153 ].…”

Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression

“…For the CIII, the treatment with JPE induced a significant increase in the activity, showing that the JPE can modulate the ETC activity. Corroborating our study, Gambato et al (2018) demonstrated an increase in CI activity in hyperglycemic endothelial cells, and the treatment with Pleurotus albidus extract, rich in phenolic compounds, was able to bring CI activity to the basal levels. Mustata et al (2005) also demonstrated that streptozotocin-induced diabetic rats showed a decrease in the specific activity of CIII in renal cortex and suggested that glycation may damage the CIII proteins.…”

Characterization of the Nutritional Composition of a Biotechnologically Produced Oyster Mushroom and its Physiological Effects in Obese Zucker Rats

“…[ 16,44 ] Mushrooms were recently discovered to be the primary source of ergothioneine which is known for its antioxidant properties. [ 45,46 ] Ergothioneine content of PSC was in range with the concentrations reported for other mushrooms, but lower than those reported for fruiting bodies of the Pleurotus species. [ 45 ] The chemical composition of mushrooms varies, according to the species, growth conditions and composition of the culture medium, which may be the reason for the variability of the chemical composition of PSC seen in this study.…”