<i>Parmelia sulcata</i> Taylor and <i>Usnea filipendula</i> Stirt induce apoptosis‐like cell death and <scp>DNA</scp> damage in cancer cells

Arı, Ferda; Aztopal, Nazlıhan; Oran, Seyhan; Bozdemir, Şefika Elmas; Çelikler, Serap; Öztürk, Şule; Ulukaya, Engin

doi:10.1111/cpr.12123

Cited by 22 publications

(22 citation statements)

References 18 publications

(26 reference statements)

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“…There is two posible splanations for that question. First, that there is cytokeratin-18 in brain; and this has been found in a study of patients with pituitary adenomas [ 36 ], and in a study of rats with glioma [ 37 ]. In the study by Luiciani et al was found CCCK-18 in cell extracts of patients with pituitary adenomas, and the use of octreotide induced apoptosis in cells of growth hormone-secreting tumors assessed by the increased of CCCK-18 in cell extracts [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the study by Luiciani et al was found CCCK-18 in cell extracts of patients with pituitary adenomas, and the use of octreotide induced apoptosis in cells of growth hormone-secreting tumors assessed by the increased of CCCK-18 in cell extracts [ 36 ]. In the study by Adri et al was found CCCK-18 in cell extracts of glioma from rats, and the use of Parmelia sulcata Taylor (one of the most common lichens that lives mainly in the bark of the trees) induced apoptosis in cell tumors assessed by the increased of CCCK-18 in cell extracts [ 37 ]. Second, that MMCAI may cause a systemic inflammatory response syndrome (SIRS), and this could activate sistemic cellular apoptosis.…”

Section: Discussionmentioning

confidence: 99%

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High serum levels of caspase-cleaved cytokeratin-18 are associated with malignant middle cerebral artery infarction patient mortality

et al. 2018

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BackgroundThere have been found apoptotic changes in brain tissue samples from humans after cerebral ischemia. Caspase-cleaved cytokeratin (CCCK)-18 could appears in blood during apoptosis. High circulating levels of CCCK-18 have been associated with a poor prognosis in patients with cerebral process, such as traumatic brain injury and spontaneous cerebral hemorrhage. However, they have not been explored in patients with ischemic stroke. Thus, the aim of this study was to determine whether there is an association between serum CCCK-18 levels and mortality in patients with severe malignant middle cerebral artery infarction (MMCAI).MethodsThis was an observational, prospective and multicentre study. We included patients with severe MMCAI. We considered MMCAI as severe when Glasgow Coma Scale (GCS) was lower than 9. We measured serum CCCK-18 levels at the diagnosis moment of the severe MMCAI.ResultsWe found that non-surviving severe MMCAI patients (n = 33) showed lower GCS and platelet count, and higher serum CCCK-18 levels than survivor ones (n = 33). We found an area under the curve (AUC) of serum CCCK-18 levels to predict 30-day mortality of 82% (95% CI = 71%–91%; p < 0.001). In the multiple logistic regression analysis was found that serum CCCK-18 levels were associated with 30-day mortality (OR = 1.023; 95% CI = 1.010–1.037; p = 0.001) after to control for platelet count and GCS.ConclusionsTo our knowledge, this is the first series reporting data on serum CCCK-18 levels in ischemic stroke patients. The novel findings of our study were that non-surviving severe MMCAI patients had higher serum CCCK-18 levels than surviving patients, and that there is an association between high serum CCCK-18 levels and MMCAI patients mortality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

High serum levels of caspase-cleaved cytokeratin-18 are associated with malignant middle cerebral artery infarction patient mortality

et al. 2018

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“…We found that the lichen species induced apoptosis-like cell death by causing DNA damage to cancer cells (Ari et al, 2014a). The antigrowth effects of UFE may arise from its secondary metabolites.…”

Section: Cytotoxic Activities Of Ufe By the Mtt And Atp Assaysmentioning

confidence: 83%

Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Kasimoğullari¹,

Oran

Arı³

et al. 2014

Turk J Biol

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Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDA-MB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 µg/mL) higher than that required for apoptosis. These results suggest that U. filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.

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“…Moreover, methanol extract of Lobothallia alphoplaca and Melanelia disjuncta exhibited high cytotoxicity against both cancer cell lines [99]. Additionally, treatment of A549, PC-3, Hep3B, and C6 cell lines with methanol extracts of Parmelia sulcata Taylor and Usnea filipendula Stirt led to the cytotoxic, genotoxic and apoptosis-inducing effects in all tested cell lines [100]. Another in vitro experiment of methanol extract of Hypogymnia physodes (low concentrations) indicated anticancer and/or apoptosis-inducing effects on MCF-7 and MDA-MB-231 cells and the genotoxic (high concentrations) impact on human lymphocytes [101].…”

Section: Determination Of Lichens' Extracts Effect Against Cancer Cellsmentioning

confidence: 91%

Anticancer Potential of Lichens’ Secondary Metabolites

et al. 2020

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Lichens produce different classes of phenolic compounds, including anthraquinones, xanthones, dibenzofuranes, depsides and depsidones. Many of them have revealed effective biological activities such as antioxidant, antiviral, antibiotics, antifungal, and anticancer. Although no clinical study has been conducted yet, there are number of in vitro and in vivo studies demonstrating anticancer effects of lichen metabolites. The main goal of our work was to review most recent published papers dealing with anticancer activities of secondary metabolites of lichens and point out to their perspective clinical use in cancer management.

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Parmelia sulcata Taylor and Usnea filipendula Stirt induce apoptosis‐like cell death and DNA damage in cancer cells

Abstract: In this study, methanolic extracts of Parmelia sulcata and Usnea filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.

Cited by 22 publications

References 18 publications

High serum levels of caspase-cleaved cytokeratin-18 are associated with malignant middle cerebral artery infarction patient mortality

High serum levels of caspase-cleaved cytokeratin-18 are associated with malignant middle cerebral artery infarction patient mortality

Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Anticancer Potential of Lichens’ Secondary Metabolites

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