Paris polyphylla var. yunnanensis genotype classification and fluorescence visual identification

Abstract

Paris polyphylla Smith. var. yunnanensis (Franch.) Hand.-Mazz (PPY) is the legal species of Rhizoma Paridis recorded in the Chinese pharmacopeia. Therefore, accurate and convenient identification of PPY and its adulterants is key to the quality control of Rhizoma Paridis. In this research, according to single nucleotide polymorphism of internal transcribed spacer, two genotypes (YN-I and YN-II) of PPY are in existence. To avoid false-negative results from the omission of the genotype during authenticity identification of PPY, two pairs of specific primers for YN-I and YN-II were designed, respectively. Furthermore, with the fluorescent dye SYBR Green I introduced into the polymerase chain reaction (PCR) system, the identification results were directly observed in virtue of the green fluorescent color of the PCR products.After methodological validation based on 25 batches of commercial medicinal material, a rapid and specific visual fluorescent identification method for two genotypes of PPY from its adulterants was established. This research also provided a model for accurate and intuitive identification of traditional Chinese medicine from multiple species of the same genus.

Agricultural sciences and the environment: Reviewing recent technologies and innovations to combat the challenges of climate change, environmental protection, and food security

“…Yin et al. (2022) reported that identification of Paris polyphylla variety yunnanensis and its adulterants is the key to the quality control of Rhizoma Paridis and visual fluorescent identification can be successfully used to discern two genotypes of Paris polyphylla variety yunnanensis .…”

“…Another paper related to the root bacterial community showed that microbial taxa could be integrated into the management strategy of wheat stripe rust . Yin et al (2022) reported that identification of Paris polyphylla variety yunnanensis and its adulterants is the key to the quality control of Rhizoma Paridis and visual fluorescent identification can be successfully used to discern two genotypes of Paris polyphylla variety yunnanensis. studied the relationship between NAC062 and NAC103 (recognized as one of the largest specific transcription factor families in plants) and they stated that it is beneficial to understand their function in biotic and abiotic plant stress in order to improve plants tolerance under environmental stresses.…”