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            -Aminobenzoic Acid Biosynthesis Is Required for Listeria monocytogenes Growth and Pathogenesis

Zhang, Yingying; Anaya-Sanchez, Andrea; Portnoy, Daniel A.

doi:10.1128/iai.00207-22

Cited by 6 publications

(3 citation statements)

References 67 publications

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“…DHP is then glutamylated with glutamate, and reduced by dihydrofolate reductase to yield the biologically active coenzyme tetrahydrofolate (THF). Finally, polyglutamate folate synthetase adds multiple glutamate moieties to THF to yield THF-polyglutamate [4,33]. Subsequent steps can be taken to synthesize other products of the folate family, such as 5-methyltetrahydrofolate [34].…”

Section: Paba and Its Derivativesmentioning

confidence: 99%

“…It serves as a precursor for certain compounds in nature, including alkaloids and indole-3-acetic acid [3]. PABA is a key intermediate in the biosynthesis of folate [4]. MABA is an essential pharmaceutical intermediate and it also finds significant applications in the synthesis of novel organic luminescent materials and plant growth regulators [5].…”

Section: Introductionmentioning

confidence: 99%

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A New Direction for the Green, Environmentally Friendly and Sustainable Bioproduction of Aminobenzoic Acid and Its Derivatives

Xiao,

Zeng,

Wang

et al. 2024

Sustainability

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Aminobenzoic acid and its derivatives are a class of aromatic compounds that are important foundational chemicals for various dyes, food additives, and pharmaceuticals. Their production relies on chemical synthesis using petroleum-derived substances such as benzene as precursors, but due to the toxicity, environmental pollution, and non-renewable nature of raw materials in chemical synthesis, some suitable alternative methods are gradually being developed. Green, environmentally friendly, and sustainable biosynthesis methods have gradually been favored by researchers, especially after the discovery of the synthetic pathways of aminobenzoic acid and its derivatives in plants and microorganisms. Based on the purpose of protecting the ecological environment, reducing the use of non-renewable resources, and providing theoretical support for industrial green development, this article reviews the biosynthesis pathways of ortho-aminobenzoic acid, meta-aminobenzoic acid, para-aminobenzoic acid, and its derivatives such as catechol, folic acid, etc., and lists some examples of biosynthesis, analyzes their advantages and disadvantages, summarizes and looks forward to the future development direction of biosynthesis of aminobenzoic acid and its derivatives.

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Section: Paba and Its Derivativesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Direction for the Green, Environmentally Friendly and Sustainable Bioproduction of Aminobenzoic Acid and Its Derivatives

Xiao,

Zeng,

Wang

et al. 2024

Sustainability

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“…In Streptococcus pneumoniae , deletion of pab , a gene responsible for pABA synthesis, attenuates virulence in mouse models of disease (Chimalapati et al., 2011). It has additionally been found in Listeria monocytogenes that mutations in the pabA and pabBC genes result in diminished intracellular growth within macrophages and significantly reduced virulence in mice (Zhang et al., 2022). This trend can even be found outside of bacteria, as disrupting pabA inhibits the ability of Aspergillus fumigatus to cause a lethal infection in a murine model of invasive pulmonary aspergillosis (Brown et al., 2000).…”

Section: Ocm and Microbial Pathogenicitymentioning

confidence: 99%

One‐carbon metabolism and microbial pathogenicity

Stocke

Lamont

2023

Molecular Oral Microbiology

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One‐carbon metabolism (OCM) pathways are responsible for several functions, producing a number of one‐carbon unit intermediates (formyl, methylene, methenyl, methyl) that are required for the synthesis of various amino acids and other biomolecules such as purines, thymidylate, redox regulators, and, in most microbes, folate. As humans must acquire folate from the diet, folate production is a target for antimicrobials such as sulfonamides. OCM impacts the regulation of microbial virulence such that in a number of instances, limiting the availability of para‐aminobenzoic acid (pABA), an essential OCM precursor, causes a reduction in pathogenicity. Porphyromonas gingivalis, however, displays increased pathogenicity in response to lower pABA levels, and exogenous pABA exerts a calming influence on heterotypic communities of P. gingivalis with pABA‐producing partner species. Differential responses to pABA may reflect both the physiology of the organisms and their host microenvironment. OCM plays an integral role in regulating the global rate of protein translation, where the alarmones ZMP and ZTP sense insufficient stores of intracellular folate and coordinate adaptive responses to compensate and restore folate to sufficient levels. The emerging interconnections between OCM, protein synthesis, and context‐dependent pathogenicity provide novel insights into the dynamic host–microbe interface.

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RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection

Stamm,

McFarland,

Locke

et al. 2024

Preprint

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Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo is challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here we have developed a murine model with a disruption in Akr1c13 that renders the resulting RECON-/- mouse resistant to high dose infection. We leveraged this model to perform a Tn-seq screen of the human pathogen Listeria monocytogenes in vivo. We identified 139 genes which were required for L. monocytogenes growth in mice including novel genes not previously identified for host survival. We identified organ specific requirements for L. monocytogenes survival and investigated the role of the folate enzyme FolD in L. monocytogenes liver pathogenesis. A mutant lacking folD was impaired for growth in murine livers by 2.5-log10 compared to wildtype and failed to spread cell-to-cell in fibroblasts. In contrast, a mutant in alsR, which encodes a transcription factor that represses an operon involved in D-allose catabolism, was attenuated in both livers and spleens of mice by 4-log10 and 3-log10, respectively, but showed modest phenotypes in in vitro models. We confirmed that dysregulation of the D-allose catabolism operon is responsible for the in vivo growth defect, as deletion of the operon in the ΔalsR background rescued virulence. By undertaking an unbiased, genome-wide screen in mice, we have identified novel fitness determinants for L. monocytogenes host infection, which highlights the utility of the RECON-/- mouse model for future screening efforts.

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para -Aminobenzoic Acid Biosynthesis Is Required for Listeria monocytogenes Growth and Pathogenesis

Cited by 6 publications

References 67 publications

A New Direction for the Green, Environmentally Friendly and Sustainable Bioproduction of Aminobenzoic Acid and Its Derivatives

A New Direction for the Green, Environmentally Friendly and Sustainable Bioproduction of Aminobenzoic Acid and Its Derivatives

One‐carbon metabolism and microbial pathogenicity

RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection

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