RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection
Chelsea E. Stamm,
Adelle P. McFarland,
Melissa N. Locke
et al.
Abstract:Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo is challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here we have developed a murine model with a disruption in Akr1c13 that renders the resulting RECON-/- mouse resistant to high dose infection. We leveraged this model to perform a Tn-seq screen of the human pathogen Li… Show more
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