Oldenlandia diffusaPromotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis

Abstract

Oldenlandia diffusa (OD) is commonly used with various diseases such as cancer, arthritis, and autoimmune disease. Liver cirrhosis is a predominant risk factor for hepatocellular carcinoma (HCC). Here, we show that the therapeutic effect of OD, which was investigated both in vitro and chemically, induced HCC model. OD significantly enhanced apoptosis and antiproliferative activity and reduced migration ability of HCC cells. In vivo, OD was treated twice a day for 28 days after confirmed HCC model through 2-[18F]-fluoro-2-deoxy-D-glucose (18F-FDG) imaging. The survival in OD treated groups was shown to have a greater therapeutic effect than the control group. 28 days after OD treatment, OD treated groups resulted in a significant reduction in tumor number, size, 18F-FDG uptake, and serum levels such as alanine transaminase, aspartate transaminase, and alkaline phosphate compared to the control group. Also, proliferated cells in tumor sites by OD were reduced compared to the control group. Furthermore, several rats in OD treated group survived over 60 days and liver morphology of these rats showed the difference between tumor mass and normal tissue. These results suggest that OD may have antiproliferative activity, inhibition of metastasis, and apoptotic effects in chemically induced HCC model and can have the potential use for clinical application as anticancer drug of the herbal extract.

Ethanol Extract of Oldenlandia diffusa Herba Attenuates Scopolamine-Induced Cognitive Impairments in Mice via Activation of BDNF, P-CREB and Inhibition of Acetylcholinesterase

“…Recently, anti-amnestic activity has been reported from natural products such as pine needle extract [ 19 ], Avena sativa extract [ 20 ] and Actinidia argute extract [ 21 ]. The extract of Oldenlandia diffusa is reported to have anti-inflammatory [ 22 ] and anti-cancer activity in colorectal [ 23 ], liver [ 24 ] and breast cancers [ 25 ]. Furthermore, its compounds such as ursolic acid and oleanolic acid were reported for their anti-amnestic and neuroprotective effects [ 26 , 27 ].…”

A Potential Herbal Adjuvant Combined With a Peptide-Based Vaccine Acts Against HPV-Related Tumors Through Enhancing Effector and Memory T-Cell Immune Responses

“…Furthermore, some previous studies demonstrated that H. diffusa Willd can induce apoptosis in ovarian cancer cells, leading to the inhibition of tumor growth in hepatocellular carcinoma (15,16). To verify this, we tested the viability of TC-1 cells treated with BHSSC.…”

“…Among frequently used forms of CHM, Hedyotis diffusa Willd (Bai Hua She She Cao, BHSSC) is associated with a reduced hazard ratio for mortality risk in nasopharyngeal carcinoma and breast cancer (9,12,14). Moreover, previous investigations have shown that H. diffusa Willd possesses important pharmacological activities and is capable of inducing apoptosis in ovarian cancer cells and inhibiting cell proliferation in hepatocellular carcinoma (15,16). However, the role of H. diffusa Willd in the regulation of the immune response and its therapeutic effect on HPV-related cancer have not been investigated.…”

Promotion of Cell Death in Cisplatin-Resistant Ovarian Cancer Cells through KDM1B-DCLRE1B Modulation

“…Based on HPLC analysis, MOD has 15 times and 5 times greater amounts of UA and OA, respectively, compared to WOD. OD extract has been reported to include asperuloside, E-6-O-p-coumaroyl scandoside methyl ester, and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether, in addition to UA and OA [6]. Individual and combined treatment with UA and OA showed relatively mild cytotoxic effects compared to MOD itself, suggesting that other compounds in MOD might contribute to MOD effects on cell viability.…”

“…Ursolic acid and oleanolic acid have been reported to have anti-tumor, apoptotic, antioxidant, cytotoxic, and anti-angiogenic activity, and anti-inflammatory effects [4,5]. Extracts of OD have also been reported to have anticancer effects [6,7]. Treatment of human breast cancer MCF-7 cells with OD extracts induced cell death through increased expression and activation of apoptosis-related proteins [8].…”