Ocimum basilicumaffects tracheal responsiveness, lung inflammatory cells and oxidant–antioxidant biomarkers in sensitized rats

Abstract

The anti-inflammatory and antioxidant effects of Ocimum basilicum (O. basilicum) was shown previously. In the present study, the effect of O. basilicum on tracheal responsiveness (TR) to methacholine and ovalbumin (OVA), bronchoalveolar lavage fluid (BALF) levels of oxidant-antioxidant biomarkers as well as total and differential white blood cell (WBC) in sensitized rats was examined. Six groups of rats including control (group C), sensitized rats to OVA (group S), S groups treated with three concentrations of O. basilicum (0.75, 1.50, and 3.00 mg/ml) and one concentration of dexamethasone (1.25 μg/ml) (n = 8 for all groups) were studied. TR to methacholine and OVA, total WBC count, percentages of eosinophils, monocytes, neutrophils, and levels of oxidant biomarkers were significantly increased but other measured parameters were significantly decreased in group S compared to group C. TR to methacholine and OVA, percentages of eosinophils, monocytes, neutrophils, and levels of oxidant biomarkers were significantly decreased but lymphocytes and antioxidant biomarkers were significantly increased in S groups treated with dexamethasone and at least two higher concentrations of the extract compared to group S. Total WBC count was also decreased in treated S groups with dexamethasone and high extract concentration. The effect of extract on most measured parameters was significantly lower than dexamethasone treatment. The effects of two higher concentrations of the extract on most variables were significantly higher than the effect of low extract concentration. These results showed the concentration-dependent effect of O. basilicum on tracheal responses, lung inflammatory cells, and oxidant-antioxidant parameters in sensitized rats.

Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma

“…The effect of the extract on immunological changes including IFN-γ, IL-4, IgE, PLA2 and TP levels and the ratio of IFN-γ/IL4 as an index of Th1/Th2 as well its effect on pathological changes were shown in the current study. In previous studies, the effect of O. basilicum extract on depression like behavior, tracheal responsiveness, lung inflammatory cells and oxidant-antioxidant biomarkers in the OA-sensitized rats was shown [23, 25, 26] which support the findings of the present studies. However, this study has some limitations such as the assessment of qualitative and quantitative extract composition which should be examined in further studies.…”

The Effect of Curcuma longa on Inflammatory Mediators and Immunological, Oxidant, and Antioxidant Biomarkers in Asthmatic Rats

“…Previously, increased total WBC and neutrophil and eosinophil percentage in lung lavage of OVA-sensitized rats were shown [23,26]. Increased percentages of eosinophils were also demonstrated in sensitized animals and asthmatic patients that correlated with the severity of asthma and supported the results of the present study [27].…”

“…Total WBC was counted in 1 ml BALF after staining with Turk solution in duplicate using a hemocytometer as previously described [22]. Under light microscopy, differential WBC was determined using morphological criteria by counting 100 cells and calculating the percentage of each cell type in a smear of the cells stained with Wright-Giemsa [23].…”

“…e products of NO metabolism (NO 2− /NO 3− ) [24], malondialdehyde (MDA) levels [25], superoxide-dismutase (SOD) [21], and catalase (CAT) [23] activities, and total thiol concentration [20] in the BALF supernatant were measured using previously described methods.…”

<p>Zona hambat ekstrak etil asetat daun kemangi (Ocimum basilicum) terhadap Enterococcus faecalis ATCC 29212</p><p>Inhibition zone of ethyl acetate extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212</p>

“…bacilicum) merupakan tanaman asli daerah tropis Asia yang umum digunakan sebagai bumbu masakan dan tanaman hias. 13,14,15,16,17,18 Makade, et al 19 menjelaskan bahwa minyak esensial O. bacilicum telah digunakan sejak lama untuk pengobatan dan pencegahan penyakit gigi dan mulut karena memiliki daya antibakteri. Vlase, et al 14 Pengujian zona hambat dengan metode difusi cakram, dilakukan dengan meletakkan 7 buah cakram uji berdiameter 6 mm yang telah ditetesi ekstrak dan kontrol pada permukaan media Mueller-Hinton Agar yang telah diinokulasikan suspensi bakteri.…”