Abstract:O-GlcNAcylation is involved in many biological processes including cancerization. Nevertheless, its in-situ quantification in single living cells is still a bottleneck. Here we develop a quantitative SERS imaging strategy for...
“…MicroRNA, an endogenous, noncoding, single-stranded RNA composed of 20–24 nucleotides, can regulate gene expression at the translation level. − Among them, MicroRNA-221 (miRNA-221) has abnormal expression in many malignant tumors, such as hepatocellular carcinoma (HCC), so it is of great significance for clinical diagnosis to achieve efficient and sensitive detection of miRNA-221. Among many detection methods such as fluorescence, electrochemiluminescence, isothermal amplification strategies, − and surface-enhanced Raman scattering (SERS), , SERS is used extensively in environmental, chemical, biomedical, and other fields due to its high sensitivity and unique optical properties, − which has powerful fingerprint information with sensitivity of as low as the single-molecule level. − …”
Herein, a surface-enhanced Raman scattering (SERS) biosensor was constructed by gold nanobipyramid (Au NBP) hotspot aggregation-induced SERS (HAI-SERS) for the ultrasensitive detection of . Impressively, compared with single Au NBP, the multiple Au NBPs assembled by tetrahedral DNA nanostructures (TDNs) could increase hotspot aggregation to significantly enhance the SERS signal of Raman molecule methylene blue (MB). Meanwhile, in the aid of Exo-III assisted target cycle amplification and TDNs-induced catalytic hairpin assembly (CHA) amplification, the biosensor could achieve the sensitive detection of miRNA-221 with a linear range of 1 fM−10 nM, and the limit of detection (LOD) was 0.59 fM, which could be used for practical application in MHCC-97L and MCF-7 cell lysates. This work provided a method for hotspot aggregation to enhance SERS for the detection of biomarkers and disease diagnosis.
“…MicroRNA, an endogenous, noncoding, single-stranded RNA composed of 20–24 nucleotides, can regulate gene expression at the translation level. − Among them, MicroRNA-221 (miRNA-221) has abnormal expression in many malignant tumors, such as hepatocellular carcinoma (HCC), so it is of great significance for clinical diagnosis to achieve efficient and sensitive detection of miRNA-221. Among many detection methods such as fluorescence, electrochemiluminescence, isothermal amplification strategies, − and surface-enhanced Raman scattering (SERS), , SERS is used extensively in environmental, chemical, biomedical, and other fields due to its high sensitivity and unique optical properties, − which has powerful fingerprint information with sensitivity of as low as the single-molecule level. − …”
Herein, a surface-enhanced Raman scattering (SERS) biosensor was constructed by gold nanobipyramid (Au NBP) hotspot aggregation-induced SERS (HAI-SERS) for the ultrasensitive detection of . Impressively, compared with single Au NBP, the multiple Au NBPs assembled by tetrahedral DNA nanostructures (TDNs) could increase hotspot aggregation to significantly enhance the SERS signal of Raman molecule methylene blue (MB). Meanwhile, in the aid of Exo-III assisted target cycle amplification and TDNs-induced catalytic hairpin assembly (CHA) amplification, the biosensor could achieve the sensitive detection of miRNA-221 with a linear range of 1 fM−10 nM, and the limit of detection (LOD) was 0.59 fM, which could be used for practical application in MHCC-97L and MCF-7 cell lysates. This work provided a method for hotspot aggregation to enhance SERS for the detection of biomarkers and disease diagnosis.
“…As well-known, redox potential is closely associated with the electron transfer driven by thermodynamic force in any given cellular state. , Alternatively, with unique function in electron transfer systems, quinones/hydroquinones-based redox-sensitive probes have been designed to respond to biologically relevant redox potentials, or concentrations of ROS/RNS and antioxidants. , Instead of the quinone/hydroquinone moieties covalently attached to various organic fluorophores that are susceptible to autofluorescence, , their surface-enhanced Raman scattering (SERS)-based probes are considered as promising candidates, thanks to the intrinsic advantages of SERS including resistant to photobleaching, high specificity and sensitivity, noninvasiveness, and so on. − Nevertheless, limited SERS-based approaches have been conducted to satisfy the demand of dynamic and high-resolution monitoring of the intracellular redox potential in different pathophysiological states.…”
Redox potential plays a key role in regulating intracellular signaling pathways, with its quantitative analysis in individual cells benefiting our understanding of the underlying mechanism in the pathophysiological events. Here, a metal organic framework (MOF)-functionalized SERS nanopotentiometer has been developed for the dynamic monitoring of intracellular redox potential. The approach is based on the encapsulation of zirconium-based MOF (Uio-66-F 4 ) on a surface of gold−silver nanorods (Au−Ag NRs) that is modified with the newly synthesized redox-sensitive probe orthomercaptohydroquinone (HQ). Thanks to size exclusion of MOF as the chemical protector, the nanopotentiometer can be adapted to long-term use and possess high anti-interference ability toward nonredox species. Combining the superior fingerprint identification of SERS with the electrochemical activity of the quinone/hydroquinone, the nanopotentiometer shows a reversible redox responsivity and can quantify redox potential with a relatively wide range of −250−100 mV. Furthermore, the nanopotentiometer allows for dynamic visualization of intracellular redox potential changes induced by drugs' stimulation in a high-resolution manner. The developed approach would be promising for offering new insights into the correlation between redox potential and tumor proliferation-involved processes such as oxidative stress and hypoxia.
“…18 Our group has also developed functional gold nanoprobe systems to image SA or protein-specic glycans on the cell surface 19,20 and quantication of intracellular glycans. 21 However, all these methods cannot achieve in vivo detection of SAs due to the inherent defects in design. Furthermore, they need large instruments such as a Raman spectrometer and confocal Raman microscope, which limits the clinical applications in diagnosis and therapy.…”
A dual gold nanoprobe system was designed for in vivo portable Raman detection of sialic acid (SA) for tumor identification. The dual gold nanoprobe system contained two gold nanoprobes, Au10-DTTC/PEG-PBA...
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