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2022
DOI: 10.1039/d2sc03881a
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O-GlcNAcylation mapping of single living cells by in situ quantitative SERS imaging

Abstract: O-GlcNAcylation is involved in many biological processes including cancerization. Nevertheless, its in-situ quantification in single living cells is still a bottleneck. Here we develop a quantitative SERS imaging strategy for...

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Cited by 9 publications
(4 citation statements)
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“…MicroRNA, an endogenous, noncoding, single-stranded RNA composed of 20–24 nucleotides, can regulate gene expression at the translation level. Among them, MicroRNA-221 (miRNA-221) has abnormal expression in many malignant tumors, such as hepatocellular carcinoma (HCC), so it is of great significance for clinical diagnosis to achieve efficient and sensitive detection of miRNA-221. Among many detection methods such as fluorescence, electrochemiluminescence, isothermal amplification strategies, and surface-enhanced Raman scattering (SERS), , SERS is used extensively in environmental, chemical, biomedical, and other fields due to its high sensitivity and unique optical properties, which has powerful fingerprint information with sensitivity of as low as the single-molecule level. …”
Section: Introductionmentioning
confidence: 99%
“…MicroRNA, an endogenous, noncoding, single-stranded RNA composed of 20–24 nucleotides, can regulate gene expression at the translation level. Among them, MicroRNA-221 (miRNA-221) has abnormal expression in many malignant tumors, such as hepatocellular carcinoma (HCC), so it is of great significance for clinical diagnosis to achieve efficient and sensitive detection of miRNA-221. Among many detection methods such as fluorescence, electrochemiluminescence, isothermal amplification strategies, and surface-enhanced Raman scattering (SERS), , SERS is used extensively in environmental, chemical, biomedical, and other fields due to its high sensitivity and unique optical properties, which has powerful fingerprint information with sensitivity of as low as the single-molecule level. …”
Section: Introductionmentioning
confidence: 99%
“…As well-known, redox potential is closely associated with the electron transfer driven by thermodynamic force in any given cellular state. , Alternatively, with unique function in electron transfer systems, quinones/hydroquinones-based redox-sensitive probes have been designed to respond to biologically relevant redox potentials, or concentrations of ROS/RNS and antioxidants. , Instead of the quinone/hydroquinone moieties covalently attached to various organic fluorophores that are susceptible to autofluorescence, , their surface-enhanced Raman scattering (SERS)-based probes are considered as promising candidates, thanks to the intrinsic advantages of SERS including resistant to photobleaching, high specificity and sensitivity, noninvasiveness, and so on. Nevertheless, limited SERS-based approaches have been conducted to satisfy the demand of dynamic and high-resolution monitoring of the intracellular redox potential in different pathophysiological states.…”
Section: Introductionmentioning
confidence: 99%
“…18 Our group has also developed functional gold nanoprobe systems to image SA or protein-specic glycans on the cell surface 19,20 and quantication of intracellular glycans. 21 However, all these methods cannot achieve in vivo detection of SAs due to the inherent defects in design. Furthermore, they need large instruments such as a Raman spectrometer and confocal Raman microscope, which limits the clinical applications in diagnosis and therapy.…”
Section: Introductionmentioning
confidence: 99%