<i>Nocardia kruczakiae</i>
            sp. nov., a Pathogen in Immunocompromised Patients and a Member of the “
            <i>N. nova</i>
            Complex”

Conville, Patricia S.; Brown, June M.; Steigerwalt, Arnold G.; Lee, Judy W.; Anderson, Victoria; Fishbain, Joel T.; Holland, Steven M.; Witebsky, Frank G.

doi:10.1128/jcm.42.11.5139-5145.2004

Cited by 47 publications

(31 citation statements)

References 17 publications

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“…The 99.3% similarity of the 16S rRNA genes of isolates 7, 8, and 9 to the 16S rRNA gene sequence of the type strain of N. blacklockiae is also less than the 16S rRNA gene sequence similarity of some distinct species to each other. For example, the species N. kruczakiae and N. veterana and the species N. paucivorans and N. brevicatena show 99.8 and 99.5% similarity to each other, respectively (5,25). The results of a BLAST (Basic Local Alignment Search Tool; NCBI, Bethesda, MD) analysis of the 16S rRNA gene sequences of isolates 7, 8, and 9 indicate that the next most similar type strains to these isolates are those of N. transvalensis and N. wallacei, with 98.6 and Յ98.4% similarities, respectively, which are significantly less than the similarities of these isolates to the N. blacklockiae type strain.…”

Section: Discussionmentioning

confidence: 99%

Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the “ Nocardia transvalensis Complex”

et al. 2008

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Nocardia isolates that share the property of in vitro amikacin resistance are grouped together by some authors in the Nocardia transvalensis complex. Our examination of 13 isolates that are amikacin resistant has revealed the existence of three distinct species. Sequence analysis of the 16S rRNA, 65-kDa heat shock protein, and secA1 genes, coupled with DNA-DNA hybridization, indicated that “ N. asteroides drug pattern IV,” “ N. transvalensis new taxon 1,” and N. transvalensis sensu stricto should each be considered a distinct species. The phenotypic and molecular characteristics of the proposed new species Nocardia wallacei ( N. asteroides drug pattern IV) and N. blacklockiae ( N. transvalensis new taxon 1) are presented and compared with those of N. transvalensis sensu stricto. The relative genetic diversity of isolates best placed with the species N. blacklockiae is also discussed. Case studies demonstrating the pathogenicity of N. wallacei and N. blacklockiae are presented. The type strain of N. wallacei is ATCC 49873 (DSM 45136), and that of N. blacklockiae is ATCC 700035 (DSM 45135).

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Section: Discussionmentioning

confidence: 99%

Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the “ Nocardia transvalensis Complex”

et al. 2008

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“…Currently, 16S rRNA gene sequencing is considered a better method for identification of Nocardia to the species level (Borriello et al, 2007), although certain isolates that have been found to have a high sequence similarity (99.8 %) from 16S rRNA sequences are recorded as distinct species by DNA-DNA hybridization (Conville et al, 2004;Rodríguez-Nava et al, 2006). McTaggart et al (2010) applied multilocus sequence analysis to 190 clinical isolates of Nocardia spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of clinical Nocardia isolates from India

Rudramurthy

Honnavar

Kaur

et al. 2015

Journal of Medical Microbiology

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The epidemiology of nocardiosis is evolving with increasing number of Nocardia spp. causing human infection. In recent years, molecular techniques have been used to identify Nocardia spp. There are limited data available on the spectrum of Nocardia spp. isolated from clinical samples in India. Here, a molecular study was carried on 30 clinical isolates maintained in our National Culture Collection to evaluate the techniques used for identifying the agents. The isolates were identified by sequencing two promising genes: the 16S rRNA gene and hsp65. Both hsp65 and the 16S rRNA gene could reliably identify 90 % of Nocardia isolates, i.e. N. farcinica, N. cyriacigeorgica, N. brasiliensis, N. otitidiscaviarum, N. amamiensis and N. pneumoniae. The mean percentage dissimilarity of sequence identification was higher using the hsp65 gene (4 %, range 0-7.9 %) compared with the 16S rRNA gene (2.3 %, range 0-8.9 %). Two isolates that showed ambiguous results in both the short segment of the 16S rRNA gene and hsp65 sequences could be resolved by sequencing a larger fragment (,1000 bp) of the 16S rRNA gene. Both of these isolates were identified as N. beijingensis with similarities of 99.8 and 100 % compared with the standard strain. Genotyping of N. cyriacigeorgica strains was performed using hsp65 gene sequences and compared with previously described genotypes. Our N. cyriacigeorgica isolates belonged to genotype 1 (n54) and genotype 2 (n52). The present study highlights a wide spectrum of Nocardia spp. in India and emphasizes the need for molecular techniques for identification to the species level.

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“…Nowadays, taxonomy of this genus has undergone considerable changes due to modern molecular techniques, such as identification by 16S ribosomal RNA gene sequencing. New species have been described, such as N. cryacigeorgica (Schlaberg et al, 2008), N. veterana (Pottumarthy et al, 2003), N. abscessus (Yassin et al, 2000), N. paucivorans (Eisenblatter et al, 2002) and N. kruczakiae (Conville et al, 2004), which should be taken into account in case of isolation in LTx samples.…”

Section: General Aspectsmentioning

confidence: 99%

Lung Diseases - Selected State of the Art Reviews

Irusen¹

2012

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Nocardia kruczakiae sp. nov., a Pathogen in Immunocompromised Patients and a Member of the “ N. nova Complex”

Abstract: Molecular methodologies have become useful techniques for the identification of pathogenic

Cited by 47 publications

References 17 publications

Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the “ Nocardia transvalensis Complex”

Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the “ Nocardia transvalensis Complex”

Molecular identification of clinical Nocardia isolates from India

Lung Diseases - Selected State of the Art Reviews

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