<i>ngl9</i>: A Third MADS Box Gene Expressed in Alfalfa Root Nodules

Zucchero, Joseph C.; Caspi, Michal; Dunn, Kathleen

doi:10.1094/mpmi.2001.14.12.1463

Cited by 28 publications

(29 citation statements)

References 16 publications

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“…It has been suggested that nodule MADS box proteins in M. sativa are involved in defining or maintaining the differentiated state of the nodule organ (Heard et al, 1997;Zucchero et al, 2001). In situ hybridization demonstrated that the genes encoding these proteins are expressed in infected cells, and it has been suggested that two MADS box proteins, Nmh7 and Ngl9, form heterodimers, since homo/ heterodimerization of MADS box proteins is a prerequisite for DNA binding (Zucchero et al, 2001). In this study, we identified one MADS box gene that was induced during the L. japonicus nodulation process.…”

Section: Discussionmentioning

confidence: 99%

A Positive Regulatory Role for LjERF1 in the Nodulation Process Is Revealed by Systematic Analysis of Nodule-Associated Transcription Factors of Lotus japonicus

et al. 2008

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We have used reverse genetics to identify genes involved in legume-rhizobium symbiosis in Lotus japonicus. We obtained the sequences of 20 putative transcription factors from previously reported large-scale transcriptome data. The transcription factors were classified according to their DNA binding domains and patterns of expression during the nodulation process. We identified two homologues of Medicago truncatula MtHAP2-1, which encodes a CCAAT-binding protein and has been shown to play a role in nodulation. The functions of the remaining genes in the nodulation process have not been reported. Seven genes were found to encode proteins with AP2-EREBP domains, six of which were similar to proteins that have been implicated in ethylene and/or jasmonic acid signal transduction and defense gene regulation in Arabidopsis (Arabidopsis thaliana). We identified a gene, LjERF1, that is most similar to Arabidopsis ERF1, which is up-regulated by ethylene and jasmonic acid and activates downstream defense genes. LjERF1 showed the same pattern of up-regulation in roots as Arabidopsis ERF1. The nodulation phenotype of roots that overexpressed LjERF1 or inhibited LjERF1 expression using an RNA interference construct indicated that this gene functions as a positive regulator of nodulation. We propose that LjERF1 functions as a key regulator of successful infection of L. japonicus by Mesorhizobium loti.

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Section: Discussionmentioning

confidence: 99%

A Positive Regulatory Role for LjERF1 in the Nodulation Process Is Revealed by Systematic Analysis of Nodule-Associated Transcription Factors of Lotus japonicus

et al. 2008

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“…as Alfalfa nmh7 and ngl9 genes, are also expressed in infected nodule cells (Zucchero et al, 2001) and could have a role in the development of nodules. The expression of VFUL-L and VAP1 in the grapevine lateral meristems and later in tendrils and inflorescences could, on the other hand, reflect an ancestral role of both genes in the specification and proliferation of these meristems and their derivatives, as has been previously proposed for the ancestral AP1 and AG-related gene lineages (Irish, 2003).…”

Section: Discussionmentioning

confidence: 99%

Floral Meristem Identity Genes Are Expressed during Tendril Development in Grapevine

et al. 2004

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To study the early steps of flower initiation and development in grapevine (Vitis vinifera), we have isolated two MADS-box genes, VFUL-L and VAP1, the putative FUL-like and AP1 grapevine orthologs, and analyzed their expression patterns during vegetative and reproductive development. Both genes are expressed in lateral meristems that, in grapevine, can give rise to either inflorescences or tendrils. They are also coexpressed in inflorescence and flower meristems. During flower development, VFUL-L transcripts are restricted to the central part of young flower meristems and, later, to the prospective carpel-forming region, which is consistent with a role of this gene in floral transition and carpel and fruit development. Expression pattern of VAP1 suggests that it may play a role in flowering transition and flower development. However, its lack of expression in sepal primordia, does not support its role as an A-function gene in grapevine. Neither VFUL-L nor VAP1 expression was detected in vegetative organs such as leaves or roots. In contrast, they are expressed throughout tendril development. Transcription of both genes in tendrils of very young plants that have not undergone flowering transition indicates that this expression is independent of the flowering process. These unique expression patterns of genes typically involved in reproductive development have implications on our understanding of flower induction and initiation in grapevine, on the origin of grapevine tendrils and on the functional roles of AP1-and FUL-like genes in plant development. These results also provide molecular support to the hypothesis that Vitis tendrils are modified reproductive organs adapted to climb.

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“…A, Neighbor-joining tree of B-class MADS-box deduced polypeptides from selected species: PsPI (this study; AY842491); MsNGL9 (Zucchero et al, 2001; accession no. AAK77938); AmGLO (Trö bner et al, 1992; accession no.…”

Section: Constitutive Expression Of Pspi In Tobaccomentioning

confidence: 99%

Functional Conservation of PISTILLATA Activity in a Pea Homolog Lacking the PI Motif

et al. 2005

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Current understanding of floral development is mainly based on what we know from Arabidopsis (Arabidopsis thaliana) and Antirrhinum majus. However, we can learn more by comparing developmental mechanisms that may explain morphological differences between species. A good example comes from the analysis of genes controlling flower development in pea (Pisum sativum), a plant with more complex leaves and inflorescences than Arabidopsis and Antirrhinum, and a different floral ontogeny. The analysis of UNIFOLIATA (UNI) and STAMINA PISTILLOIDA (STP), the pea orthologs of LEAFY and UNUSUAL FLORAL ORGANS, has revealed a common link in the regulation of flower and leaf development not apparent in Arabidopsis. While the Arabidopsis genes mainly behave as key regulators of flower development, where they control the expression of B-function genes, UNI and STP also contribute to the development of the pea compound leaf. Here, we describe the characterization of P. sativum PISTILLATA (PsPI), a pea MADS-box gene homologous to B-function genes like PI and GLOBOSA (GLO), from Arabidopsis and Antirrhinum, respectively. PsPI encodes for an atypical PI-type polypeptide that lacks the highly conserved C-terminal PI motif. Nevertheless, constitutive expression of PsPI in tobacco (Nicotiana tabacum) and Arabidopsis shows that it can specifically replace the function of PI, being able to complement the strong pi-1 mutant. Accordingly, PsPI expression in pea flowers, which is dependent on STP, is identical to PI and GLO. Interestingly, PsPI is also transiently expressed in young leaves, suggesting a role of PsPI in pea leaf development, a possibility that fits with the established role of UNI and STP in the control of this process.A huge variety of inflorescence and floral morphologies are found among higher plants. Increasing attention is currently being paid to the study of the mechanisms responsible for this natural diversity. Recent advances in plant molecular genetics have allowed detailed comparative studies on how the development of equivalent organs and structures is regulated in different plants. An important conclusion of these studies is that most of the genetic functions that establish the general pattern for organ specification are executed by orthologous genes in the different species and, more importantly, that the observed morphological variation is achieved in a large degree through the modification of the function of those regulators in each particular species (Theissen et al., 2000).A clear example of conservation is the specification of the identity of floral organs. The classical ABC model proposes that floral organ identity results from the action of three genetic functions, A, B, and C, each of them active in two adjacent whorls of organs. Afunction alone specifies sepal identity, A plus B petal identity, B plus C stamens, and C alone carpels. The ABC model was developed on the basis of studies carried out in the model plants Arabidopsis (Arabidopsis thaliana) and Antirrhinum majus (Coen and Meyerowitz, 1991), two ...

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ngl9: A Third MADS Box Gene Expressed in Alfalfa Root Nodules

Cited by 28 publications

References 16 publications

A Positive Regulatory Role for LjERF1 in the Nodulation Process Is Revealed by Systematic Analysis of Nodule-Associated Transcription Factors of Lotus japonicus

A Positive Regulatory Role for LjERF1 in the Nodulation Process Is Revealed by Systematic Analysis of Nodule-Associated Transcription Factors of Lotus japonicus

Floral Meristem Identity Genes Are Expressed during Tendril Development in Grapevine

Functional Conservation of PISTILLATA Activity in a Pea Homolog Lacking the PI Motif

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