<i>Neospora caninum</i>infection in an aborted bovine foetus in Brazil

Gondim, Luís Fernando Pita; Sartor, Izidoro Francisco; Monteiro, L.A.R.C.; Haritani, Makoto

doi:10.1080/00480169.1999.36106

Cited by 18 publications

(14 citation statements)

References 11 publications

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“…No Brasil, Gondim et al (1999), detectaram, pelo método de imunoistoquímica, o N. caninum em um feto abortado de 8 meses, cujo título de anticorpos no soro foi de 1:6400. Nessa propriedade, com histórico de abortamentos, do soro de dez vacas submetidos à RIFI, seis foram positivos com títulos de 1:800 (três), 1:1600 (dois), 1:3200 (um), sendo que a literatura considera como específicos, títulos iguais ou superiores a 200 DRAKE, 1996).…”

Section: Introductionunclassified

Ocorrência de anticorpos de Neospora caninum em vacas leiteiras avaliados pelos métodos ELISA e RIFI no município de Avaré, SP

Sartor

Hasegawa

Canavessi³

et al. 2003

Sem. Ci. Agr.

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ResumoSoros de 521 vacas do município de Avaré, SP, foram analisados pelos métodos de reação de imunofluorescência indireta (RIFI) e teste imunoenzimático (ELISA) para a detecção de anticorpos contra Neospora caninum. As amostras reagentes para Neospora também foram submetidas à RIFI para Toxoplasma gondii. Pelo método de RIFI 15,9% das amostras foram positivas para N. caninum nas diluições de 1:200 a 1:1600 e pelo teste de ELISA 30,5% dos soros foram positivos. Das amostras positivas para ELISA e negativas para a RIFI 1:200, 46 foram reagentes na diluição de 1:160. Todas as 129 vacas positivas para RIFI nas diluições ≥ 1:160 também o foram para ELISA. Dos 44 abortamentos constatados, 14 (31,8%) e 18 (40,9%) vacas apresentaram título na RIFI ≥ 1:200 e ≥ 1:160, respectivamente, e 24 (54,5%) reagiram ao teste de ELISA. Dos 159 soros que reagiram para N. caninum nos testes de RIFI e/ou ELISA, apenas seis foram positivos para T. gondii pelo método de RIFI e, das 44 vacas com histórico de abortamentos, apenas uma apresentou anticorpos anti-Toxoplasma. Esses resultados sugerem a presença de N. caninum nos rebanhos estudados e sua relação com os abortamentos observados. Palavras-chave: Neosporose, bovinos, Apicomplexa. AbstractSera of 521 dairy cows from herds in Avaré County, SP were examined by indirect fluorescent-antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Neospora caninum. The positive samples for Neospora were also tested for Toxoplasma gondii by IFAT. By using IFAT 15.9% of the samples reacted for N. caninum with dilution between 1:200 and 1: 1600. By using ELISA 30.5% were seropositive for Neospora. Of the samples that were positive to ELISA and negative to IFAT for antibodies titers of 1:200, 46 showed IFAT titers of 1:160. All 129 cows that were seropositive to IFAT with titers of 1:200 and 1:160 were also positive to ELISA. Out of 44 detected abortions, 14 (31.8%) and 18 (40.9%) cows had IFAT titers of ≥ 1:200 and ≥ 1:160, respectively, and 24 (54.5%) cows were positive to ELISA. Of the 159 seropositive samples to N. caninum to IFAT and/or ELISA, only six were positive to IFAT to T. gondii. Moreover, among the 44 cows with history of abortion, only one demonstrated antibodies to Toxoplasma. These results suggest the presence of N. caninum in the herds that were evaluated and the potential relationship with the observed abortions.

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Section: Introductionunclassified

Ocorrência de anticorpos de Neospora caninum em vacas leiteiras avaliados pelos métodos ELISA e RIFI no município de Avaré, SP

Sartor

Hasegawa

Canavessi³

et al. 2003

Sem. Ci. Agr.

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“…More recent investigations have reported rates of 7.7% in Mato Grosso do Sul (ANDREOTTI et al, 1999), 14.09% in Bahia (GONDIM et al, 1999a, 11.2% in Rio Grande do Sul (CORBELLINI et al, 2002), 14.3% in Paraná (GUIMARÃES et al, 2004, 12.61% in Minas Gerais (MELO et al, 2004), 8,8% in Rondônia (AGUIAR et al, 2006 and as much as 30.4% in Goiás (MELO et al, 2006). The parasite has been found in cattle fetuses both in São Paulo and in Rio Grande do Sul (GONDIM et al, 1999b;CORBERLLINI et al, 2002). Strains have been isolated from dogs in Bahia (GONDIM et al, 2001), cattle fetuses in Paraná (LOCATELLI-DITTRICH et al, 2003), and buffaloes from São Paulo (RODRIGUES et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of anti-Neospora caninum antibodies in cattle from the state of Mato Grosso do Sul, Brazil

Oshiro

Matos²,

Oliveira³

et al. 2007

Rev. Bras. Parasitol. Vet.

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[Prevalência de anticorpos anti-Neospora caninum em rebanhos bovinos de Mato Grosso do Sul, Brasil.] Revista Brasileira de Parasitologia Veterinária, v. 16, n. 3, p. 133-138, 2007. Embrapa Beef Cattle, BR 262, km 4, Caixa Postal 154, Campo Grande, MS 79002-970, Brazil. E-mail: andreott@cnpgc.embrapa.br Neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, as well as ruminants and equines. It was described in 1988 and has been known as a major cause of abortion in bovines and neuromuscular alterations and death in dogs. To estimate the prevalence of bovine neosporosis in the 22 municipalities of the so-called Estrato 1 subregion of the Brazilian state of Mato Grosso do Sul, blood samples were collected from cows aged 24 months and older, from December 2003 to March 2004. During sample collection, a questionnaire was used to gather data of epidemiological interest. The samples were subjected to serological diagnosis (indirect fluorescence antibody test -IFAT). Prevalences of 14.9% (449/2488) and 69.8% (143/205) were found for the animals and herds sampled, respectively. The variable found to be associated with seropositivity to N. caninum was abortion (OR 2.52; CI 1.25-5.06). The results revealed the presence of infection by N. caninum in the herds investigated, drawing attention to its role as a potential cause of abortion in cattle in Mato Grosso do Sul.

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“…The first identification of N. caninum in an aborted fetus in Brazil was performed by Gondim et al (1999). Santos et al (2005) examined 34 individuals (fetuses and stillbirths) from Paraná State histologically and detected 8 positive for N. caninum using immunohistochemistry (IHC).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

Cabral¹,

Camargo²,

Galleti³

et al. 2009

RBPV

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Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were examined for evidence of N. caninum. Histological examination revealed 71.4% with non-suppurative inflammation in the heart, lung, liver, kidney, placenta, and brain. Immunohistochemistry detected infections in 8.6% of the samples, mainly in the brain, placenta, and heart. Nested-PCR JB revealed 6.7% with infections, while nested-PCR PLUS returned 20.9% positive results, mainly in brain and placenta, and in the pooled liver and heart. Kappa statistics demonstrated little agreement among the three techniques. The three methods are complementary, since they have distinct diagnostic characteristics and were combined to give a positivity rate of 24.8%.

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Neospora caninuminfection in an aborted bovine foetus in Brazil

Cited by 18 publications

References 11 publications

Ocorrência de anticorpos de Neospora caninum em vacas leiteiras avaliados pelos métodos ELISA e RIFI no município de Avaré, SP

Ocorrência de anticorpos de Neospora caninum em vacas leiteiras avaliados pelos métodos ELISA e RIFI no município de Avaré, SP

Prevalence of anti-Neospora caninum antibodies in cattle from the state of Mato Grosso do Sul, Brazil

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

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