No abstract

The tendency of dogs infected with E canis to bleed may be related not only to thrombocytopenia but also to platelet dysfunction associated with the disease.

ResumoSoros de 521 vacas do município de Avaré, SP, foram analisados pelos métodos de reação de imunofluorescência indireta (RIFI) e teste imunoenzimático (ELISA) para a detecção de anticorpos contra Neospora caninum. As amostras reagentes para Neospora também foram submetidas à RIFI para Toxoplasma gondii. Pelo método de RIFI 15,9% das amostras foram positivas para N. caninum nas diluições de 1:200 a 1:1600 e pelo teste de ELISA 30,5% dos soros foram positivos. Das amostras positivas para ELISA e negativas para a RIFI 1:200, 46 foram reagentes na diluição de 1:160. Todas as 129 vacas positivas para RIFI nas diluições ≥ 1:160 também o foram para ELISA. Dos 44 abortamentos constatados, 14 (31,8%) e 18 (40,9%) vacas apresentaram título na RIFI ≥ 1:200 e ≥ 1:160, respectivamente, e 24 (54,5%) reagiram ao teste de ELISA. Dos 159 soros que reagiram para N. caninum nos testes de RIFI e/ou ELISA, apenas seis foram positivos para T. gondii pelo método de RIFI e, das 44 vacas com histórico de abortamentos, apenas uma apresentou anticorpos anti-Toxoplasma. Esses resultados sugerem a presença de N. caninum nos rebanhos estudados e sua relação com os abortamentos observados. Palavras-chave: Neosporose, bovinos, Apicomplexa. AbstractSera of 521 dairy cows from herds in Avaré County, SP were examined by indirect fluorescent-antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Neospora caninum. The positive samples for Neospora were also tested for Toxoplasma gondii by IFAT. By using IFAT 15.9% of the samples reacted for N. caninum with dilution between 1:200 and 1: 1600. By using ELISA 30.5% were seropositive for Neospora. Of the samples that were positive to ELISA and negative to IFAT for antibodies titers of 1:200, 46 showed IFAT titers of 1:160. All 129 cows that were seropositive to IFAT with titers of 1:200 and 1:160 were also positive to ELISA. Out of 44 detected abortions, 14 (31.8%) and 18 (40.9%) cows had IFAT titers of ≥ 1:200 and ≥ 1:160, respectively, and 24 (54.5%) cows were positive to ELISA. Of the 159 seropositive samples to N. caninum to IFAT and/or ELISA, only six were positive to IFAT to T. gondii. Moreover, among the 44 cows with history of abortion, only one demonstrated antibodies to Toxoplasma. These results suggest the presence of N. caninum in the herds that were evaluated and the potential relationship with the observed abortions.

No abstract

A total of 222 dogs were examined by blood smear examination and Hepatozoon canis infection was detected in 13 dogs (5.9%). Five H. canis-infected dogs were necropsied to observe tissue stages in the organs. Fragments of spleen, liver, lungs, heart, kidneys, lymph nodes, bone marrow and skeletal muscles were used to made touch-impression smears. No macroscopic lesions were found in the organs. Two dogs had gamonts within polymorphonuclear cells and schizonts in various stages of development within the spleen and the bone marrow. Nevertheless, no mature meronts were found.

No abstract

Infection by E canis has no influence on neutrophil oxidative metabolism even though during the remission period of the acute phase of the disease, the neutrophils seem to be more reactive under stimulation.

To evaluate the anthelmintic effect of Musa spp. leaves, 12 animals were artificially infected with Haemonchus contortus, and another 12 animals were infected with Trichostrongylus colubriformis. Then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. During the trials, the animals received weekly physical examinations. The methods used to evaluate the efficiency of this treatment were packed cell volume, total plasma protein and faecal egg counts, and egg hatchability tests were performed on days -2, +3, +6, +9, +13 and +15. Coproculture tests were performed on day -2 to confirm monospecific infections. In the FEC and EHT, a statistically significant difference (0.04, 0.005; p < 0.05) was noted for T. colubriformis. There were no statistically significant differences (p > 0.05) for Haemochus contortus group in all tests. Our results confirmed previous findings suggesting that dried ground banana plant leaves possess anthelmintic activity.