<i>N</i>‐dihydrogalactochitosan as a potent immune activator for dendritic cells

El-Hussein, Ahmed; Lam, Samuel Siu Kit; Raker, Joseph; Chen, Wei R.; Hamblin, Michael R.

doi:10.1002/jbm.a.35991

Cited by 20 publications

(10 citation statements)

References 50 publications

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“…The red region of the electromagnetic spectrum in particular can stimulate many anti-oxidant enzymatic activities, like catalase, ceruloplasmin, and superoxide dismutase [ 48 ]. Those effects could be vital for many biological systems through their stimulating actions on other proliferative, metabolic, and homeostatic processes [ 49 , 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

The Prospective Beneficial Effects of Red Laser Exposure on Lactocaseibacillus casei Fermentation of Skim Milk

Mohamed

Elshaghabee

Alharbi

et al. 2020

Biology

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Probiotic lactic acid bacteria are crucial producers of fermented dairy products that are popular functional foods in many countries. The health benefits of probiotic bacteria are mainly attributed to their effective bioactive metabolites. The quality of fermented milk is mainly dependent on the bacterial strain used in the fermentation process. In this study, an innovative technique is used in order to enhance the activities of the probiotic bacteria, quality of fermented milk, and consequently the whole fermentation process. Red laser dosages, at the wavelength of 632.7 nm, were applied to the type strain Lacticaseibacillus casei NRRL-B-1922 before the fermentation of skim milk. The results revealed that the scavenging of 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical and total antioxidant capacity were significantly increased from 21% in untreated control to 56% after bacterial laser irradiation of 12 J/cm2 dosage for 40 min. The antioxidant activity was found to be increased as the red laser dosage increased in a dose-response relationship. Additionally, the lactose fermentation in skim milk medium of 43.22 mg/mL initial concentration into organic acids was enhanced after L. casei irradiation and recorded 23.15 mg/mL compared to control group 28.35 mg/mL without bacterial pre-treatment. These results are correlated with increase of the β-Galactosidase activity, where the L. casei that has been exposed to 40 min of red laser exhibited the higher activity of a 0.37 unit/mL relative to the control 0.25 unit/mL. The assessment of this fermented milk after L. casei laser exposure for 10, 20, and 40 min indicates multiple biological effects, including assimilation of cholesterol as well as proteolytic and antibacterial activity. Our data on the exposure of L. casei to laser beam suggest promising application of red laser in the fermentation process of skim milk.

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Section: Resultsmentioning

confidence: 99%

The Prospective Beneficial Effects of Red Laser Exposure on Lactocaseibacillus casei Fermentation of Skim Milk

Mohamed

Elshaghabee

Alharbi

et al. 2020

Biology

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“…The nanoscopic morphological alterations that have been seen from the standard and modern normal bones suggest that collagen fibrils and in consequence the mineral crystals underwent major organizational and structural adjustments. The exact sequence of the changes that occur to the bones may be not well understood due to the biomarkers' losses in the course of diagenesis (El-Hussein et al, 2017). A broad idea of what could happen on the archeological bones could be described by the disorganization of collagen before hydroxyapatite loses its crystal appearance and consequently disintegrates along with collagen decay.…”

Section: Tem Resultsmentioning

confidence: 99%

Investigating Egyptian archeological bone diagenesis using ATR-FTIR microspectroscopy

Kasem

Yousef

Alrowaili

et al. 2020

Journal of Radiation Research and Applied Sciences

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Bones are mainly composite materials of equivalent volume fractions of mineral (apatite) and organic (collagen) parts. Their infrared spectroscopic characteristics mirror their composition. Bone diagenesis is the post-depositional changes of calcified tissues by chemical degradation. It is a complicated process and affected by numerous external and individual factors. Diagenetic trajectories have been employed using Fourier transform infrared (FTIR) spectroscopy to monitor the preservation status of bioapatite. We studied the diagenesis in ancient Egyptian bones of four dynasties collected from two different locations -Sakkara and Aswan using attenuated total reflectance (ATR) FTIR microspectroscopy. Despite the latter is a promising technique since it is a minimally destructive tool, the samples' physical properties may affect the data accuracy. The studied bone pieces were from two sites, the shaft and the femur head. Transmission electron microscopy (TEM) was used to investigate the bone crystals size and shape. ATR-FTIR spectrographs showed different molecular fingerprints between Sakkara and Aswan soil samples. The technique was able to monitor different molecular structures across the shaft bones indicating different diagenetic grades. TEM micrographs showed low abundant crystals of needle shaped for Late period shaft bones, while those from Roman Greek period were characterized with abundant irregular thin platelet crystals.

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“…It also could be phagocytosed by DCs and remained in DCs for a long time (Fig. S4) (28). In the treated tumor tissues, uptake of injected GC-FITC by infiltrating DCs was confirmed by flow cytometry (Fig.…”

Section: Resultsmentioning

confidence: 99%

Local Phototherapy Synergizes with Immunoadjuvant for Treatment of Pancreatic Cancer through Induced Immunogenic Tumor Vaccine

Zhou

Yang

Zhang

et al. 2018

Clinical Cancer Research

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To develop a synergistic combination therapy for advanced pancreatic cancer, using local phototherapy and immunotherapy, and to determine the efficacy and mechanism of the novel combination therapy using a highly metastatic pancreatic tumor model in mice. Mice bearing Panc02-H7 pancreatic tumors (both subcutaneous and orthotopic) were treated with noninvasive or interventional photothermal therapy, followed by local application of an immunoadjuvant. Tumor growth and animal survival were assessed. Immune cell populations within spleen and tumors were evaluated by FACS and IHC, and cytokine levels were determined by ELISA. Up to 75% of mice bearing subcutaneous tumors treated with combination therapy had complete tumor regression. Local photothermal therapy exposed/released damage-associated molecular patterns, which initiated an immunogenic tumor cell death, resulting in infiltration of antigen-presenting cells and Th1 immunity. Concomitant application of immunoadjuvant amplified Th1 immunity, especially the tumor-specific cytotoxic T lymphocyte response, with increased quantity and quality of T cells. Combination therapy also induced tumor-specific immune memory, as demonstrated by resistance to tumor rechallenge and production of memory T cells. For the treatment of orthotopic tumor, the combination therapy significantly reduced the primary tumors and metastases, and prolonged the animal survival time. This study indicated that combination of local phototherapy and immunotherapy induced a systemic immunity against established tumors and metastases in an aggressive, preclinical pancreatic tumor model, leading to a potential clinical method for patients with advanced pancreatic cancer. .

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N‐dihydrogalactochitosan as a potent immune activator for dendritic cells

Cited by 20 publications

References 50 publications

The Prospective Beneficial Effects of Red Laser Exposure on Lactocaseibacillus casei Fermentation of Skim Milk

The Prospective Beneficial Effects of Red Laser Exposure on Lactocaseibacillus casei Fermentation of Skim Milk

Investigating Egyptian archeological bone diagenesis using ATR-FTIR microspectroscopy

Local Phototherapy Synergizes with Immunoadjuvant for Treatment of Pancreatic Cancer through Induced Immunogenic Tumor Vaccine

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