N‐acylhomoserine lactone‐regulation of genes mediating motility and pathogenicity in Pseudomonas syringae pathovar tabaci 11528

Cheng, Feifei; Ma, Anzhou; Luo, Ji; Zhuang, Xuliang; Zhuang, Guoqiang

doi:10.1002/mbo3.440

Cited by 8 publications

(4 citation statements)

References 74 publications

(128 reference statements)

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“…The breadth of host range that we observed was consistent with other traits that have been reported to be well correlated with pathogenic potential-such as motility, the function of certain transmembrane proteins and the products of biosynthetic clusters. Swarming motility has been shown to be an important means for plant associated bacteria to assure invasion of plant tissue and to move to new nutritional reservoirs (summarized in (Venieraki et al 2016)) and to be positively correlated with pathogenicity in individual strains as revealed by mutants that are altered in their ability to swarm (Ichinose et al 2016;Cheng et al 2017). This is consistent with our observation that strains with the broadest host range tend to be those that swarm, and conversely those that do not swarm tend to have narrower host ranges.…”

Section: Discussionmentioning

confidence: 99%

The overlapping continuum of host range among strains in the Pseudomonas syringae complex

et al. 2019

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Pseudomonas syringae is the most frequently emerging group of plant pathogenic bacteria. Because this bacterium is ubiquitous as an epiphyte and on various substrates in non-agricultural settings, there are many questions about how to assess the risk for plant disease posed by strains in the environment. Although P. syringae is considered to have discrete host ranges in defined pathovars, there have been few reports of comprehensive comparisons of host range potential. Here we present results of host range tests for 134 strains, representing eight phylogroups, from epidemics and environmental reservoirs on 15 to 22 plant species per test conducted in four separate tests to determine the patterns and extent of host range. We sought to identify trends that are indicative of distinct pathotypes and to assess if strains in the P. syringae complex are indeed restricted in their host range. We show that for each test, strains display a diversity of host ranges from very restricted to very broad regardless of the gamut of phylogroups used in the test. Overall, strains form an overlapping continuum of host range potential with equal representation of narrow, moderate and broad host ranges. Groups of distinct pathotypes, including strains with currently the same pathovar name, could not be identified. The absence of groupings was validated with statistical tests for pattern recognition. The extent of host range was positively correlated with the capacity of strains to swarm on semi-solid agar medium and with the abundance of genes in biosynthetic clusters and was inversely correlated with the abundance of genes for proteins with transmembrane domains in their genomes. Our results are consistent with the current paradigm that disease symptoms are the result of multiple molecular interactions between P. syringae and its plant host that are modulated by abiotic and biotic conditions. This leads us to propose that pathovar denominations do not correspond to the underlying biology of P. syringae. A new concept of pathogenicity that accounts for the continuum of pathogenic potential in P. syringae would open new perspectives to understand the evolution of pathogenicity in this bacterium and new insights to anticipate disease and to manage plant health.

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Section: Discussionmentioning

confidence: 99%

The overlapping continuum of host range among strains in the Pseudomonas syringae complex

et al. 2019

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“…syringae strain B728a, AHL QS regulates the transcription of only a nine-gene cluster located adjacent to the ahlR-ahlI locus, which also contains the pdhT and pdhQ loci (28), whereas in Pseudomonas syringae pv. tabaci strain 11528, over 300 genes were found to be regulated by QS, including phdT, pdhQ, and the pssR homologs (35). Despite such a difference in AHL QS regulons among these strains, the transcription of pdhT, pdhQ, and pssR (ahlR) is common in all P. syringae species, and their role in P. syringae deserves further attention (36).…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: Role in Virulence and Interspecies Interactions in the Olive Knot

Caballo-Ponce

Meng

Uzelac

et al. 2018

Appl Environ Microbiol

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The olive knot disease ( L.) is caused by the bacterium pv. savastanoi. pv. savastanoi in the olive knot undergoes interspecies interactions with the harmless endophyte ; pv. savastanoi and colocalize and form a stable community, resulting in a more aggressive disease. pv. savastanoi and produce the same type of the-acylhomoserine lactone (AHL) quorum sensing (QS) signal, and they share AHLs In this work, we have further studied the AHL QS systems of pv. savastanoi and in order to determine possible molecular mechanism(s) involved in this bacterial interspecies interaction/cooperation. The AHL QS regulons of pv. savastanoi and were determined, allowing the identification of several QS-regulated genes. Surprisingly, the pv. savastanoi QS regulon consisted of only a few loci whereas in many putative metabolic genes were regulated by QS, among which are several involved in carbohydrate metabolism. One of these loci was the aldolase-encoding gene, which was found to be essential for both colocalization of pv. savastanoi and cells inside olive knots as well as knot development. This study further highlighted that pathogens can cooperate with commensal members of the plant microbiome. This is a report on studies of the quorum sensing (QS) systems of the olive knot pathogen pv. savastanoi and olive knot cooperator These two bacterial species form a stable community in the olive knot, share QS signals, and cooperate, resulting in a more aggressive disease. In this work we further studied the QS systems by determining their regulons as well as by studying QS-regulated genes which might play a role in this cooperation. This represents a unique interspecies bacterial virulence model and highlights the importance of bacterial interspecies interaction in disease.

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“…These studies demonstrated that QS mostly regulates motility ( Table 2 ). Indeed, the homologues of LuxI–LuxR (named the PsyI–PsyR system in this pathovar) repress the expression of genes involved in swarming mobility, flagellum formation, pili assembly, biofilm, and chemotaxis at the beginning of the exponential phase [ 17 , 18 ].…”

Section: Acyl Homoserine Lactone-mediated Quorum Sensingmentioning

confidence: 99%

Quorum Sensing Regulation in Phytopathogenic Bacteria

2021

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Quorum sensing is a type of chemical communication by which bacterial populations control expression of their genes in a coordinated manner. This regulatory mechanism is commonly used by pathogens to control the expression of genes encoding virulence factors and that of genes involved in the bacterial adaptation to variations in environmental conditions. In phytopathogenic bacteria, several mechanisms of quorum sensing have been characterized. In this review, we describe the different quorum sensing systems present in phytopathogenic bacteria, such as those using the signal molecules named N-acyl-homoserine lactone (AHL), diffusible signal factor (DSF), and the unknown signal molecule of the virulence factor modulating (VFM) system. We focus on studies performed on phytopathogenic bacteria of major importance, including Pseudomonas, Ralstonia, Agrobacterium, Xanthomonas, Erwinia, Xylella,Dickeya, and Pectobacterium spp. For each system, we present the mechanism of regulation, the functions targeted by the quorum sensing system, and the mechanisms by which quorum sensing is regulated.

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N‐acylhomoserine lactone‐regulation of genes mediating motility and pathogenicity in Pseudomonas syringae pathovar tabaci 11528

Cited by 8 publications

References 74 publications

The overlapping continuum of host range among strains in the Pseudomonas syringae complex

The overlapping continuum of host range among strains in the Pseudomonas syringae complex

Quorum Sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: Role in Virulence and Interspecies Interactions in the Olive Knot

Quorum Sensing Regulation in Phytopathogenic Bacteria

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