Quorum Sensing Regulation in Phytopathogenic Bacteria

Baltenneck, Julie; Reverchon, Sylvie; Hommais, Florence

doi:10.3390/microorganisms9020239

Cited by 45 publications

(28 citation statements)

References 117 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intercellular communication in bacteria, technically referred to as QS, is a critical factor in disease spread and pathogenesis because it regulates biofilm formation. Reportedly, it plays an important role in development of Dickeya -induced Potato soft rot ( Baltenneck et al, 2021 ). ANU-301 was also identified as a source of MPP and 2,6-bis(1-methyl-1-phenylethyl)-phenol by GC-MS/MS analysis as the first and third most abundant chemical compounds, respectively.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot

Choi

Ahsan

2022

Plant Pathol J

View full text Add to dashboard Cite

To screen antagonistic fungi against plant pathogens, dual culture assay (DCA) and culture filtrate assay (CFA) were performed with unknown soil-born fungi. Among the different fungi isolated and screened from the soil, fungal isolate ANU-301 successfully inhibited growth of different plant pathogenic fungi, Colletotrichum acutatum, Alternaria alternata, and Fusarium oxysporum, in DCA and CFA. Morphological characteristics and rDNA internal transcribed spacer sequence analysis identified ANU-301 as Aspergillus terreus. Inoculation of tomato plants with Fusarium oxysporum f. sp. lycopersici (FOL) induced severe wilting symptom; however, co-inoculation with ANU-301 significantly enhanced resistance of tomato plants against FOL. In addition, culture filtrate (CF) of ANU-301 not only showed bacterial growth inhibition activity against Dickeya chrysanthemi (Dc), but also demonstrated protective effect in potato tuber against soft rot disease. Gas chromatography-tandem mass spectrometry analysis of CF of ANU-301 identified 2,4-bis(1-methyl-1-phenylethyl)-phenol (MPP) as the most abundant compound. MPP inhibited growth of Dc, but not of FOL, in a dose-dependent manner, and protected potato tuber from the soft rot disease induced by Dc. In conclusion, Aspergillus terreus ANU-301 could be used and further tested as a potential biological control agent.

show abstract

Section: Discussionmentioning

confidence: 99%

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot

Choi

Ahsan

2022

Plant Pathol J

View full text Add to dashboard Cite

show abstract

“…The DSF-family chemicals are important quorum-sensing signals in Gram-negative bacteria and participate in the interkingdom communication between bacteria and fungi (5,27,28). Various DSF-family chemicals have been identified in Xanthomonas, Pseudomonas, Stenotrophomonas, and Burkholderia bacterial species, including DSF, BDSF, IDSF, CDSF, and SDSF (10,29). These chemicals are structurally similar, but their abundance, precursors, cellular dynamics, and functions have not been thoroughly investigated.…”

Section: Discussionmentioning

confidence: 99%

BDSF is a degradation-prone quorum-sensing signal detected by the histidine kinase RpfC of Xanthomonas campestris pv. campestris

Tian

Cai

et al. 2022

Preprint

View full text Add to dashboard Cite

Diffusible signal factors (DSFs) are medium-chain fatty acids that induce bacterial quorum sensing. Among these compounds, BDSF is a structural analog of DSF that is commonly detected in bacterial species (e.g., Xanthomonas, Pseudomonas, and Burkholderia). Additionally, BDSF contributes to the interkingdom communication regulating fungal life stage transitions. How BDSF is sensed in Xanthomonas spp. and the functional diversity between BDSF and DSF remain unclear. In this study, we generated genetic and biochemical evidence that BDSF is a low-active regulator of X. campestris pv. campestris quorum sensing, whereas trans-BDSF seems not a signaling compound. BDSF is detected by the sensor histidine kinase RpfC. Although BDSF has relatively low physiological activities, it binds to the RpfC sensor with a high affinity and activates RpfC autophosphorylation to a level that is similar to that induced by DSF in vitro. The inconsistency in the physiological and biochemical activities of BDSF is not due to RpfC–RpfG phosphorylation or RpfG hydrolase. Neither BDSF nor DSF controls the phosphotransferase and phosphatase activities of RpfC or the ability of RpfG hydrolase to degrade the bacterial second messenger cyclic di-GMP. We demonstrated that BDSF is prone to degradation by RpfB, a critical fatty acyl-CoA ligase involved in the turnover of DSF-family signals. rpfB mutations lead to substantial increases in BDSF-induced quorum sensing. Although DSF and BDSF are similarly detected by RpfC, our data suggest that their differential degradation in cells is the major factor responsible for the diversity in their physiological effects.

show abstract

“…AHLs are signaling molecules produced by various Gram-negative bacteria [ 17 , 18 ]. Among them, many phytopathogens use AHL-based communication [ 50 , 65 ], including a large number of rhizobial strains described as biofilm formers, in which QS plays a crucial role in the switch in lifestyle towards biofilms. This switch, governed by QS, involves modifications to the bacterial phenotype, including bacterial motility [ 66 , 67 ] and EPS production [ 68 , 69 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Biofilm Formation: Jamming of Sessile-Associated Rhizobial Communication by Rhodococcal Quorum-Quenching

Bourigault

Rodrigues

Crépin

et al. 2021

IJMS

View full text Add to dashboard Cite

Biofilms are complex structures formed by a community of microbes adhering to a surface and/or to each other through the secretion of an adhesive and protective matrix. The establishment of these structures requires a coordination of action between microorganisms through powerful communication systems such as quorum-sensing. Therefore, auxiliary bacteria capable of interfering with these means of communication could be used to prevent biofilm formation and development. The phytopathogen Rhizobium rhizogenes, which causes hairy root disease and forms large biofilms in hydroponic crops, and the biocontrol agent Rhodococcus erythropolis R138 were used for this study. Changes in biofilm biovolume and structure, as well as interactions between rhizobia and rhodococci, were monitored by confocal laser scanning microscopy with appropriate fluorescent biosensors. We obtained direct visual evidence of an exchange of signals between rhizobia and the jamming of this communication by Rhodococcus within the biofilm. Signaling molecules were characterized as long chain (C14) N-acyl-homoserine lactones. The role of the Qsd quorum-quenching pathway in biofilm alteration was confirmed with an R. erythropolis mutant unable to produce the QsdA lactonase, and by expression of the qsdA gene in a heterologous host, Escherichia coli. Finally, Rhizobium biofilm formation was similarly inhibited by a purified extract of QsdA enzyme.

show abstract

Quorum Sensing Regulation in Phytopathogenic Bacteria

Cited by 45 publications

References 117 publications

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot

BDSF is a degradation-prone quorum-sensing signal detected by the histidine kinase RpfC of Xanthomonas campestris pv. campestris

Biocontrol of Biofilm Formation: Jamming of Sessile-Associated Rhizobial Communication by Rhodococcal Quorum-Quenching

Contact Info

Product

Resources

About