<i>Mycoplasma</i> species isolated from falcons in the Middle East

Lierz, Michael; Schmidt, R. E.; Runge, Martin

doi:10.1136/vr.151.3.92

Cited by 25 publications

(33 citation statements)

References 6 publications

(6 reference statements)

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“…The local chickens appeared normal without any obvious clinical signs. This result was similar to the findings of previous authors (Carpenter et al, 1981;Lierz et al, 2000;Lierz et al, 2002;Lierz et al, 2008;Peebles et al, 2004) who found high seroprevalence of Mycoplasma in birds of prey even though the adult birds remained clinically normal. This can be attributed to the type of husbandry system practiced by the villagers and including lack of vaccination of local chickens and non-existence of mycoplasma control program in Nigeria.…”

Section: Discussionsupporting

confidence: 83%

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Ahmed¹,

Lawal²,

Fatihu³

et al. 2015

Afr. J. Cell. Path

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Aim:The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation. Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium. Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar.

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Section: Discussionsupporting

confidence: 83%

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Ahmed¹,

Lawal²,

Fatihu³

et al. 2015

Afr. J. Cell. Path

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“…This underlines that positive results from PCRs must be carefully interpreted, especially if the tests are used in avian populations such as birds of prey, where there is regular detection of unidentified Mycoplasma species (Lierz et al, 2000a(Lierz et al, , 2002(Lierz et al, , 2008a. The putative Mycoplasma species that caused the false positive results seems to be closely related to M. buteonis as it has a 98% identity with the 16S rRNA gene of M. buteonis reference strain.…”

Section: Discussionmentioning

confidence: 99%

“…The poultry pathogens M. gallisepticum, M. iowae, M. meleagridis and M. synoviae have been isolated from a variety of different hosts (Bradbury, 2006), including M. gallisepticum and M. meleagridis from birds of prey (Poveda et al, 1990a,b;Morishita et al, 1997;Lierz et al, 2000Lierz et al, , 2002. Mycoplasmas are common in birds of prey, and certain species (M. buteonis, M. gypis, M. falconis) are regularly detected in addition to a number of unidentified species (Lierz et al, 2008b).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to its usual host, the turkey, there have been repeated isolations of M. meleagridis from birds of prey (Lierz et al, 2000a(Lierz et al, , 2002, and there have also been unconfirmed reports of isolations from quail and peacocks (Simecka et al, 1992). Apart from chicken and turkey, M. synoviae was isolated from other galliformes (Benčina et al, 1987;Poveda et al, 1990b) as well as from anseriformes (Benčina et al, 1988a,b Tiong, 1990, pigeons (Benčina et al, 1987), and a house sparrow (Passer domesticus) (Poveda et al, 1990b).…”

Section: Introductionmentioning

confidence: 99%

“…In general, mycoplasmas are common in birds of prey (Morishita et al, 1997;Lierz et al, 2000bLierz et al, , 2002Lierz et al, , 2008a but the Mycoplasma species that are pathogenic for poultry are only occasionally reported. The presence of M. gallisepticum, M. iowae, M. meleagridis and M. synoviae in captive and free-ranging raptors from Germany was therefore investigated using polymerase chain reactions (PCRs).…”

Section: Introductionmentioning

confidence: 99%

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Use of polymerase chain reactions to detectMycoplasma gallisepticum,Mycoplasma imitans,Mycoplasma iowae,Mycoplasma meleagridisandMycoplasma synoviaein birds of prey

et al. 2008

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Certain Mycoplasma spp. are pathogens of poultry, but little is known of the role of mycoplasmas in disease of birds of prey. Species-specific polymerase chain reactions (PCRs) for the detection of the poultry pathogens Mycoplasma gallisepticum, Mycoplasma imitans, Mycoplasma iowae, Mycoplasma meleagridis and Mycoplasma synoviae were therefore evaluated for use in birds of prey. The specificities of the PCR methods were established using avian and other mycoplasmas and also selected walled bacteria. The sensitivities of the different PCR assays varied between 100 fg and 10 pg DNA. Fifty-three tracheal swabs from healthy captive and free-ranging birds of prey were then investigated using these PCRs, and in no case was an amplicon obtained for M. gallisepticum/M. imitans, M. iowae or M. synoviae. Species-specific primers for M. meleagridis amplified a product from eight birds of prey but restriction enzyme analysis as well as sequencing of PCR products demonstrated these results to be false positives. Alignment studies of the sequenced products with the 16S rRNA gene sequence of various Mycoplasma species in GenBank demonstrated an identity of 91% to M. meleagridis but of 98% to Mycoplasma buteonis or Mycoplasma gallopavonis. Isolation and attempted identification of these mycoplasmas suggested it may be a previously unrecognized species.

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Mycoplasma

Brown¹,

May²,

Bradbury³

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

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My.co.plas'ma. Gr. masc. n. myces a fungus; Gr. neut. n. plasma something formed or molded, a form; N.L. neut. n. Mycoplasma fungus form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Mycoplasma Pleomorphic cells, 300–800 nm in diameter, varying in shape from spherical, ovoid or flask‐shaped, or twisted rods, to slender branched filaments ranging in length from 50 to 500 nm. Cells lack a cell wall and are bounded by a single plasma membrane. Gram‐stain‐negative due to the absence of a cell wall. Some have a complex internal cytoskeleton. Some have a specific tip structure that mediates attachment to host cells or other surfaces. Usually nonmotile , but gliding motility has been demonstrated in some species. Aerobic or facultatively anaerobic . Optimum growth at 37°C is common, but permissive growth temperatures range from 20 to 45°C. Chemo‐organotrophic , usually using either sugars or arginine as the major energy source. Require cholesterol or related sterols for growth. Colonies are usually less than 1 mm in diameter. The typical colony has a fried‐egg appearance . The genome size of species examined ranges from 580 kbp to about 1350 kbp . The codon UGA encodes tryptophan in all species examined. Commensals or pathogens in a wide range of vertebrate hosts. DNA G + C content ( mol %): 23–40. Type species : Mycoplasma mycoides (Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910) Freundt 1955, 73 ( Asterococcus mycoides Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910, 179).

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Mycoplasma species isolated from falcons in the Middle East

Cited by 25 publications

References 6 publications

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Use of polymerase chain reactions to detectMycoplasma gallisepticum,Mycoplasma imitans,Mycoplasma iowae,Mycoplasma meleagridisandMycoplasma synoviaein birds of prey

Mycoplasma

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