Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Abstract

Aim:The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation. Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium. Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar.

Evaluation of Isolation and Polymerase Chain Reaction in Diagnosis of Mycoplasma Gallisepticum in Broiler Chickens in Kirkuk Governorate, Iraq

“…Difficulties with the serologic tests used in diagnosing mycoplasmas lie in the defects associated with interpreting the results [15]. PCR is a very sensitive technology capable of producing billions of copies of a specific segment of DNA for cloning, sequencing, and analysis [16], It has high specificity and sensitivity facilitating MG detection even in clinical samples taken from animals that are asymptomatic, or receiving antibiotic therapy [17], but its requires specialized laboratory facilities, an experienced workforce, and the costly nature of detecting and screening for pathogen, which limits its use in conventional laboratories, especially in the developing world [18]. The gold standard test for MG diagnosis is isolation [19], but it is expensive, laborious, and time-consuming, other bacteria including non-pathogenic Mycoplasma may contaminate it, and often Mycoplasma does not grow on the ordinary media [20].…”