<i>Mycobacterium vaccae</i>Immunization to OVA Sensitized Pregnant BALB/c Mice Suppressed Placental and Postnatal IL-5 and Inducing IFN-γ Secretion

Akkoç, Tunç; Eifan, Aarif O.; Özdemir, Cevdet; Yazi, Didem; Yesil, Ozlem; Bahceciler, Nerin N.; Barlan, Işıl

doi:10.1080/08923970701812159

Cited by 11 publications

(10 citation statements)

References 21 publications

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“…Treatment of mice with mycobacterium‐induced allergen‐specific Tregs induces IL‐10 and TGF‐β, which protect against airway inflammation . Our previous research supported the role of M. vaccae administration in preventing development of asthma during both pregnancy and the neonatal period in mice . Another recent study has shown that probiotics also have immunomodulatory properties as adjuvants with skewing of CD4 + T cells towards a Th1/possibly Treg or Th1 profile .…”

supporting

confidence: 73%

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Akkoç

Genç

Zibandeh

et al. 2018

Microbiology and Immunology

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Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used to treat allergic diseases. Mycobacteria can be used as an adjuvant for inducing T regulatory cells. However, it is unclear whether intranasal immunotherapy in combination with Mycobacteria adjuvant induces regulatory T cell differentiation and attenuates allergic responses in vivo. To investigate the role of intranasal ovalbumin (OVA) treatment alone and in combination with Mycobacteria vaccae, proportions of FoxP3 regulatory T cells and anti-inflammatory responses were evaluated in a murine model of asthma that was established in three groups of bicistronic Foxp3 reporter BALB/c mice. Before establishment of the asthma model, two groups of mice received intranasal OVA immunotherapy and one also received simultaneous s.c. M. vaccae. Expression of CD4 CD25 Foxp3 T cells in the lung and spleen was analyzed by flow cytometry and the cytokine profiles of allergen-stimulated lung and spleen lymphocytes assessed. The intranasal OVA immunotherapy group showed greater expression of CD4 CD25 Foxp3 T cells in the spleen whereas in the group that also received M. vaccae such greater expression was demonstrated in the lung. Additionally, the proportion of IL-10 and IFN-γ-secreting splenocytes was greater in the intranasal OVA + M. vaccae group. CD25 neutralization decreased CD4 Foxp3 cells more than other groups. In parallel with this finding, production of IL-10 and IFN-γ was down-regulated. Mucosal administration of OVA antigen results in a greater proportion of CD4 Foxp3 T cells in the spleen. IL-10 and IFN-γ induced by intranasal OVA immunotherapy and M. vaccae administration is down-regulated after CD25 neutralization.

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supporting

confidence: 73%

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Akkoç

Genç

Zibandeh

et al. 2018

Microbiology and Immunology

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“…29 Studies have shown that OVA sensitized by Mycobacterium vaccae immunization in pregnant mice can inhibit IL-5 production and induce IFN-γ secretion, both in the placenta and in the postpartum. 30 In this study, the polarity of Th2 in the offspring was weakened after BL gavage during pregnancy, and the Th1 polarization pathway was correspondingly enhanced. Furthermore, Treg differentiation was enhanced, whereas that of Th9 was weakened.…”

Section: Discussionmentioning

confidence: 54%

Maternal microbiome regulation prevents early allergic airway diseases in mouse offspring

Liu

et al. 2020

Pediatric Allergy Immunology

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Background: Asthma is a serious global health problem, severely affecting the lives of sufferers and their families. An exceptionally hygienic home and reduced microbial exposure can aggravate the incidence of childhood asthma. Methods: Specific-pathogen-free BALB/c mice were pre-treated with bacterial lysate (BL; 1 mg/kg) as a high microbial load maternal mouse model, and then, the offspring mice were established as an allergic airway disease (AAD) model. The expression levels of TLR2, TLR4, and HDAC9 in the mother's intestine and the offspring's lungs were detected. Relevant indicators of regulatory T cells (Tregs) were identified in the mother and offspring mice. The changes in the expression of Th1-, Th2-, Th9-, and Th17-related cytokines in the offspring mice were evaluated among different pretreated groups. Results: After augmenting the mothers' intestinal microbiota through oral BL gavage, the expression of TLR2 and TLR4 in the colon mucosa and colon lymphoid tissues was enhanced and that of HDAC9 in the colon mucosa was decreased, and the proportion of spleen Tregs was increased. The offspring showed similar changes in the AAD model compared with the offspring of the control-group mothers: TLR2 and TLR4 expression in the lungs and the proportion of spleen Tregs increased, HDAC9 expression in the lungs decreased, and AAD-induced airway pathologic characteristics were reversed; additionally, Th1/Th2 and Th9 imbalances were rectified. Conclusions: This study presents a new framework for the prevention of childhood asthma, elucidating the mechanism of regulating the mother's intestinal microbiome to protect the offspring's early asthma via animal experiments.

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“…We demonstrated that a single M. vaccae administration to OVA-sensitized pregnant mice down-regulated IL-5 secretion and up-regulated IFN-γ secretion from placental lymphocytes. Moreover, this cytokine profile persisted in newborns up to the 28 th day of life [44].…”

Section: Discussionmentioning

confidence: 84%

<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

Akkoç

Özdemir²,

Yazi³

et al. 2014

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Context: One of the treatment strategies for atopic diseases is to skew immune response away from Th2 dominance by using Mycobacterial strains. Objective: We wanted to find out whether M. vaccae administration to pregnant mice had any preventive effect on the offsprings in the development of a murine asthma model. Materials and Methods: Pregnant BALB/c mice were divided into two groups; first group received heat-killed M. vaccae subcutaneously on 12 th day of pregnancy and the latter group received PBS. After delivery, newborn mice of each group were further divided into two subgroups as M. vaccae/Ovalbumin (OVA), M. vaccae/control, PBS/OVA and PBS/ control. To establish experimental murine asthma model, mice were intraperitoneally sensitized and challenged intratracheally with Ovalbumin. We analysed airway histopathology, bone marrow eosinophil progenitors and splenic cell cytokine profiles of the offsprings. Results: Comparison of offsprings in M. vaccae/OVA group were not different than PBS controls with respect to thicknesses of airway epithelium, basement membrane, subepithelial smooth muscles and number of hyperplasic goblet cells as well as bronchial associated lymphoid tissue density and eosinophil progenitors in the bone marrow. Comparison of M. vaccae/OVA group to asthma model revealed significant differences and lower levels of OVA-induced IL-5. Conclusions: We propose that immunization of pregnant BALB/c with M. vaccae could prevent histopathological alterations in the airways related to the asthma model and down-regulates IL-5 secretion from splenocytes of offsprings.

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Mycobacterium vaccaeImmunization to OVA Sensitized Pregnant BALB/c Mice Suppressed Placental and Postnatal IL-5 and Inducing IFN-γ Secretion

Cited by 11 publications

References 21 publications

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Maternal microbiome regulation prevents early allergic airway diseases in mouse offspring

<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

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Mycobacterium vaccaeImmunization to OVA Sensitized Pregnant BALB/c Mice Suppressed Placental and Postnatal IL-5 and Inducing IFN-γ Secretion

Cited by 11 publications

References 21 publications

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Intranasal ovalbumin immunotherapy with mycobacterial adjuvant promotes regulatory T cell accumulation in lung tissues

Maternal microbiome regulation prevents early allergic airway diseases in mouse offspring

&lt;i&gt;Mycobacterium vaccae&lt;/i&gt; Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings

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<i>Mycobacterium vaccae</i> Immunization to Pregnant BALB/c Mice Ameliorated Lung Histopathology and Bone Marrow Eosinophila in Ovalbumin Sensitized Offsprings