<i>Mycobacterium tuberculosis</i> Secreted Proteins As Potential Biomarkers for the Diagnosis of Active Tuberculosis and Latent Tuberculosis Infection

Zhang, Caiqin; Song, Xiaoqin; Zhao, Yong; Hai, Zhang; Song, Zhao; Mao, Fengfeng; Bai, Bing; Wu, Shaoping; Shi, Changhong

doi:10.1002/jcla.21782

Cited by 45 publications

(42 citation statements)

References 23 publications

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“…In fact while comparing the sensitivity and specificity of our antigens with these two reference antigens, we found that antigen rSS1, rSS4 and rSS5 are highly significantly superior to the esat6 and 38 kDa Ag or other previously published studies30. In comparison to reference antigen (esat6 and 38 kDa Ag), We noticed great difference in the specificity of rSS1, rSS2, rSS3, rSS4, rSS5 antigens, though only three antigens (rSS1, rSS4, rSS5) showed significant difference in the sensitivity.…”

Section: Discussionmentioning

confidence: 56%

Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

Singh

Gupta

Gopinath

et al. 2017

Sci Rep

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Improved methods are required for the early and accurate diagnosis of tuberculosis, especially in the patients with smear-negative disease. Several biomarkers have been tried but most have shown poor sensitivity or specificity. In present study we aimed to evaluate the diagnostic utility of five novel antigens identified earlier by us. This is an initial study conducted on 250 subjects. The five recombinant antigens, named as rSS1 (Rv2145c), rSS2 (Rv0164), rSS3 (Rv1437), rSS4 (Rv1827) and rSS5 (Rv2970c), were expressed in pQE-30 expression vector, purified and their sero-diagnostic efficacy was evaluated in an unblinded manner using dot-blot and ELISA methods. The sensitivity and specificity of these novel antigens were compared with commercially available standard esat6 and 38 kDa antigens. Bacteriologically confirmed TB patients, non-TB disease controls and healthy individuals were included. which are based on novel antigen or novel technology, Area under curve (AUC) of the selected antigens were 0.98 (0.98–0.99) for rSS1, 0.88 (0.84–0.92) for rSS2, 0.88 (0.84–0.92) for rSS3, 0.95 (0.93–0.98) for rSS4 and 0.99 (0.98–1.0) for rSS5. Receiver operative characteristic (ROC) curve showed highly significant difference between TB and healthy subjects (p = <0.001). These initial findings, show that the recombinant antigens rSS1, rSS4 and rSS5 could be used as highly potential biomarkers for the serological diagnosis of active TB.

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Section: Discussionmentioning

confidence: 56%

Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

Singh

Gupta

Gopinath

et al. 2017

Sci Rep

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“…In contrast, NK cells were associated with significantly decreased antigen‐specific expression of CD107a in TB‐DM patients . In addition, Zhang et al . reported that NKT cells from peripheral blood mononuclear cells in TB patients with or without DM were significantly increased compared with levels in non‐TB diabetic patients and healthy controls.…”

Section: Innate Immunity To Tb In Patients With Dmmentioning

confidence: 98%

Influence of diabetes mellitus on immunity to human tuberculosis

2017

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SummaryType 2 diabetes mellitus(DM) is a major risk factor for the development of active pulmonary tuberculosis (TB), with development of DM pandemic in countries where TB is also endemic. Understanding the impact of DM on TB and the determinants of co-morbidity is essential in responding to this growing public health problem with improved therapeutic approaches. Despite the clinical and public health significance posed by the dual burden of TB and DM, little is known about the immunological and biochemical mechanisms of susceptibility. One possible mechanism is that an impaired immune response in patients with DM facilitates either primary infection with Mycobacterium tuberculosis or reactivation of latent TB. Diabetes is associated with immune dysfunction and alterations in the components of the immune system, including altered levels of specific cytokines and chemokines. Some effects of DM on adaptive immunity that are potentially relevant to TB defence have been identified in humans. In this review, we summarize current findings regarding the alterations in the innate and adaptive immune responses and immunological mechanisms of susceptibility of patients with DM to M. tuberculosis infection and disease.

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“…DNA vaccination with 85A encoding structure has induced the same magnitude of protection as BCG . In addition, some secretory proteins of M. tuberculosis (ESAT6, CFP10, Ag85B, Hsp16.3) and the fusion protein (Ag85B‐Hsp16.3) are proven to be biomarkers for the diagnosis of active TB and latent TB infection . Hence, identifying secretory protein in M. tuberculosis is important and urgent step for further related work.…”

Section: Introductionmentioning

confidence: 99%

SecProMTB: Support Vector Machine‐Based Classifier for Secretory Proteins Using Imbalanced Data Sets Applied to Mycobacterium tuberculosis

2019

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Secretory proteins of Mycobacterium tuberculosis have created more concern, given their dominant immunogenicity and role in pathogenesis. In view of expensive and time‐consuming traditional biochemical experiments, an advanced support vector machine model named SecProMTB is constructed in this study and the proteins are identified by a bioinformatic approach. First, an improved pseudo‐amino acid composition (PseAAC) algorithm is used to extract features from all entities. Second, a novel imbalanced‐data strategy is proposed and adopted to divide the original data set into train set and test set. Third, to overcome the overfitting problem, feature‐ranking algorithms are applied with an increment feature selection. Finally, the model is trained and optimized. Consequently, a model is obtained with an area under the curve of 0.862 and average accuracy of 86% in the independent test. For the convenience of users, SecProMTB and related data are openly accessible at http://server.malab.cn/SecProMTB/index.jsp.

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Mycobacterium tuberculosis Secreted Proteins As Potential Biomarkers for the Diagnosis of Active Tuberculosis and Latent Tuberculosis Infection

Cited by 45 publications

References 23 publications

Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

Influence of diabetes mellitus on immunity to human tuberculosis

SecProMTB: Support Vector Machine‐Based Classifier for Secretory Proteins Using Imbalanced Data Sets Applied to Mycobacterium tuberculosis

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