<i>Musca domestica</i> Pupae Lectin Improves the Immunomodulatory Activity of Macrophages by Activating Nuclear Factor-κB

Cao, Xiaohong; Zhou, Minghui; Hou, Lihua; Li, Yuanyuan; Chen, Linye

doi:10.1089/jmf.2011.1712

Cited by 5 publications

(7 citation statements)

References 41 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, Wang reported that lectins played a role in the coordination of hemagglutination, encapsulation and melanization in Helicoverpa armigera pathogen elimination . Housefly larvae infected with E. coli are reported to eliminate pathogens by activating the phenoloxidase cascade , which enhances hemagglutination and promotes cytophagocytosis through lectin (Cao et al 2012). In this study, we observed brown spots under the epidermis of larvae within 3 h of infection with C. albicans.…”

Section: Discussionsupporting

confidence: 56%

Histological Observation and Expression Patterns of antimicrobial peptides during Fungal Infection in Musca domestica (Diptera: Muscidae) Larvae

Xiu

Wang

et al. 2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

Housefly, Musca domestica, has a complicated immune system. However, its underlying operating mechanism remains elusive. Candida albicans is a major pathogen affecting humans by causing deep infection fungous disease, but it is non-symbiotic in houseflies. To investigate the C. albicans infection process in housefly, the changes in morphological and histological and expression patterns of antimicrobial peptide were monitored to indicate the insect's response to fungal infection. The results showed that scattered brown spots were comprising melanized encapsulation and encapsulated fungal cells were initially observed at the inner side of larvae's body wall 3 h postinfection (PI). Between 6 and 36 h PI, the whole body of larvae was densely covered with the brown spots, which then gradually disappeared. The majority had disappeared at 48 h PI. Some fungi colonized in the gaps between the body wall and the muscle layer, as well as among muscle fibers of the muscle layer at 12 h PI and hyphal was observed at 18 h PI. These fungi colonized distribution changed from a continuous line to scattered spots at 24 h PI and virtually disappeared at 48 h. The results of quantitative PCR analysis revealed that in coordination with the variation during the infection, the expression levels of four antimicrobial peptides were up-regulated. In conclusion, C. albicans infection in M. domestica larvae involved the following stages: injection, infection, immune response and elimination of the pathogen. The rapid response of antimicrobial peptides, melanized encapsulation and agglutination played a vital role against the pathogenic invasion.

show abstract

Section: Discussionsupporting

confidence: 56%

Histological Observation and Expression Patterns of antimicrobial peptides during Fungal Infection in Musca domestica (Diptera: Muscidae) Larvae

Xiu

Wang

et al. 2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Many studies have indicated that AMPs from the housefly possess pharmacological value (Cao et al, ; Chen et al, ). Using high‐throughput sequencing methods, seven AMP genes ( CECROPIN, ATTACIN, DEFENSIN, DIPTERICIN, MUSCIN, DOMESTICIN, and LYSOZYME ) have been found in M. domestica infected with a mixture of E. coli and S. aureus (Tang et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have indicated that AMPs from the housefly possess pharmacological value (Cao et al, 2012;Chen et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial peptides from the edible insect Musca domestica and their preservation effect on chilled pork

Dang

Zheng

Wang

et al. 2019

J Food Process Preserv

View full text Add to dashboard Cite

Antimicrobial compounds from natural sources are a promising alternative to address the consumer demand of food without chemical additives. In this study, antimicrobial peptides (AMPs) from infected Musca domestica pupae (Md‐AMPs) bred under clean conditions were isolated and prepared on a large scale. In these Md‐AMPs, seven AMPs families were identified by liquid chromatography–tandem mass spectrometry. Md‐AMPs at concentrations from 0.4 to 0.8 mg/ml exhibited antibacterial activity and showed negligible hemolytic activity against human erythrocytes. Md‐AMPs increased bacterial membrane permeability of four tested strains, resulting in the leakage of phosphorus‐containing materials and Ca2+. Md‐AMPs bound plasmid DNA and inhibited its migration in a concentration‐dependent manner. Treatment with Md‐AMPs alone or in combination with nisin, and ethylenediaminetetraacetic acid was efficient in inhibiting bacterial growth in chilled pork and extended the shelf life up to 6 days. Practical applications Insects have been widely used as foods and many biofunctional components are found in insects. In this work, we isolated and prepared AMPs from M. domestica, and its antibacterial activity, hemolytic activity, and mechanism of action were investigated. Trials in real food products showed that Md‐AMPs alone or a combination of three natural antimicrobials could remarkably inhibit bacterial growth in chilled pork. These results suggest that Md‐AMPs can be used as food preservatives.

show abstract

“…M. domestica larvae are considered an excellent source of protein, fats, minerals, vitamins, and high levels of amino acids and have been used in Chinese and Korean traditional medicine [ 7 , 8 ]. M. domestica has exhibited numerous bioactive properties such as antibacterial [ 9 ], anti-atherosclerosis and pro-inflammatory responses [ 10 ], and immunomodulatory [ 11 ], antiviral [ 12 ], antifungal [ 13 ], antioxidants, and anticancer activities [ 14 ]. The chemical composition of M. domestica larval hemolymph is very complex, mainly formed of antimicrobial peptides (AMPs), lysozyme, and agglutinin [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Curcumin-Injected Musca domestica Larval Hemolymph: Cecropin Upregulation and Potential Anticancer Effect

et al. 2022

View full text Add to dashboard Cite

Over recent decades, much attention has been given to imply the natural products in cancer therapy alone or in combination with other established procedures. Insects have a rich history in traditional medicine across the globe, which holds promise for the future of natural product drug discovery. Cecropins, peptides produced by insects, are components of a defense system against infections and are well known to exert antimicrobial and antitumor capabilities. The present study aimed to investigate, for the first time, the role of curcumin in enhancing the anticancer effect of Musca domestica larval hemolymph. Third larval instars of M. domestica were injected with curcumin and the hemolymph was picked at 4, 8, and 24 h post-curcumin injection. M. domestica cecropin A (MdCecA) was evaluated in control and injected larval hemolymphs. The cytotoxicity on breast cancer cell lines (MCF-7) and normal Vero cells was assessed to be comparable to control larval hemolymph. Curcumin-injected larval hemolymphs exhibited significant cytotoxicity with respect to the uninjected ones against MCF-7; however, Vero cells showed no cytotoxicity. The IC50 was 106 ± 2.9 and 388 ± 9.2 μg/mL for the hemolymphs of injected larvae at 4 and 8 h, respectively, while the control larval hemolymph revealed the IC50 of >500 μg/mL. For mechanistic anticancer evaluation, concentrations of 30, 60, and 100 μg/mL of curcumin-injected larval hemolymphs were examined. A significant G2/M cell cycle arrest was observed, confirming the anti-proliferative properties of hemolymphs over the tested concentrations. The MdCecA transcripts were significantly (p < 0.05) upregulated at 4 and 8 h post-injection, while a significant downregulation was observed after 24 h. Cecropin quantification by LC–MS revealed that MdCecA peptides have the highest expression in the hemolymph of the treated larvae at 8 h relative to the control group. The upregulation of cecropin expression at mRNA and protein levels may be attributed to the curcumin stimulation and linked to the increased cytotoxicity toward the cancer cell line. In conclusion, the results suggest that the apoptotic and anti-proliferative effects of M. domestica hemolymph on MCF-7 cells following the curcumin injection can be used as a natural candidate in future pharmaceutical industries.

show abstract

Musca domestica Pupae Lectin Improves the Immunomodulatory Activity of Macrophages by Activating Nuclear Factor-κB

Cited by 5 publications

References 41 publications

Histological Observation and Expression Patterns of antimicrobial peptides during Fungal Infection in Musca domestica (Diptera: Muscidae) Larvae

Histological Observation and Expression Patterns of antimicrobial peptides during Fungal Infection in Musca domestica (Diptera: Muscidae) Larvae

Antimicrobial peptides from the edible insect Musca domestica and their preservation effect on chilled pork

Curcumin-Injected Musca domestica Larval Hemolymph: Cecropin Upregulation and Potential Anticancer Effect

Contact Info

Product

Resources

About