Abstract:The objective of this study was to investigate the role of miR-148a-3p in lupus nephritis (LN) based on data from previous studies and a microRNA assay. We evaluated the miR-148a-3p expression level in LN renal tissues and blood serum to determine its clinicopathological significance and effect on glomerular cell proliferation. Then, we collected renal glomeruli from LN mice and determined the miR-148a-3p, proliferating cell nuclear antigen (PCNA), and PCNA/Thy1 expression. We performed functional analyses of … Show more
“…miR‐151‐3p functions as a key regulator in epithelial‐to‐mesenchymal transition (EMT), innate immunity, and inflammation, which is concordant with the fact that H pylori infection unavoidably triggers immune response and inflammation. In addition, miR‐148a‐3p played important roles in restraining cell proliferation and EMT . Taken together, these facts indicate that H pylori infection involves in multiple biological processes that are collectively mediated by these miRNAs.…”
Section: Discussionmentioning
confidence: 83%
“…In addition, miR-148a-3p played important roles in restraining cell proliferation and EMT. [31][32][33] Taken together, these facts indicate that H pylori infection involves in multiple biological processes that are collectively mediated by these miRNAs.…”
Helicobacter pylor (H pylori), a Gram‐negative, microaerobic human pathogen, has been found to be involved in many gastroduodenal diseases. Accurate diagnosis of H pylori infection is a vital part of the effective management of gastroduodenal diseases. Circulating microRNAs (miRNAs) have shown the potential to be used as noninvasive biomarkers for the diagnosis of infectious diseases. The aim of this study was to explore plasma miRNAs as noninvasive biomarkers for H pylori infection. We performed a plasma miRNA expression profile using Illumina high‐throughput sequencing and validated the levels of differentially expressed miRNAs in the plasma of 63 H pylori‐infected patients and 41 healthy volunteers by quantitative real‐time polymerase chain reaction (qRT‐PCR). The sequencing results showed that 37 miRNAs were upregulated in the H pylori‐infected patients compared with that in the healthy volunteers, while six miRNAs were downregulated. qRT‐PCR and receiver operator characteristic analysis suggested that the expression of miR‐28‐3p, miR‐143‐3p, miR‐151a‐3p, and miR‐148a‐3p were closely associated with H pylori infection. Therefore, the four plasma miRNA panels mentioned above could serve as promising noninvasive biomarkers of H pylori infection.
“…miR‐151‐3p functions as a key regulator in epithelial‐to‐mesenchymal transition (EMT), innate immunity, and inflammation, which is concordant with the fact that H pylori infection unavoidably triggers immune response and inflammation. In addition, miR‐148a‐3p played important roles in restraining cell proliferation and EMT . Taken together, these facts indicate that H pylori infection involves in multiple biological processes that are collectively mediated by these miRNAs.…”
Section: Discussionmentioning
confidence: 83%
“…In addition, miR-148a-3p played important roles in restraining cell proliferation and EMT. [31][32][33] Taken together, these facts indicate that H pylori infection involves in multiple biological processes that are collectively mediated by these miRNAs.…”
Helicobacter pylor (H pylori), a Gram‐negative, microaerobic human pathogen, has been found to be involved in many gastroduodenal diseases. Accurate diagnosis of H pylori infection is a vital part of the effective management of gastroduodenal diseases. Circulating microRNAs (miRNAs) have shown the potential to be used as noninvasive biomarkers for the diagnosis of infectious diseases. The aim of this study was to explore plasma miRNAs as noninvasive biomarkers for H pylori infection. We performed a plasma miRNA expression profile using Illumina high‐throughput sequencing and validated the levels of differentially expressed miRNAs in the plasma of 63 H pylori‐infected patients and 41 healthy volunteers by quantitative real‐time polymerase chain reaction (qRT‐PCR). The sequencing results showed that 37 miRNAs were upregulated in the H pylori‐infected patients compared with that in the healthy volunteers, while six miRNAs were downregulated. qRT‐PCR and receiver operator characteristic analysis suggested that the expression of miR‐28‐3p, miR‐143‐3p, miR‐151a‐3p, and miR‐148a‐3p were closely associated with H pylori infection. Therefore, the four plasma miRNA panels mentioned above could serve as promising noninvasive biomarkers of H pylori infection.
“…26 In this regard, Qingjuan et al recently reported that miR-148a could directly bind to the 3 0 -UTR of PTEN and reduce the expression of PTEN in lupus nephritis, resulting in accelerated glomerular cell proliferation and tumor progression. 27 However, to introduce miRNAs into the clinical practice, more research is needed to identify their target mRNAs and understand their functions, as they harbor both oncogenic and tumor suppressive characteristics. The different features of miRNAs suggest that they act on multiple targets in different cellular processes that contribute to PCa development and progression.…”
The aim of this study was to establish a unique expression profile of circulating cell-free microRNAs (miRNAs) capable of differentiating between prostate cancer (PCa) patients with high-risk and intermediate-risk Gleason scores. MiRNA expression profiles were determined in plasma samples from 79 treatment-naïve PCa patients, 1-2 follow-up samples after radical prostatectomy (RP) from 51 out of the 79 PCa patients, and 33 healthy men, using a quantitative real-time PCR-based array containing 48 selected miRNAs. We identified 27 up- and 2 downregulated plasma miRNAs in PCa patients compared with healthy men. Most of the upregulated miRNA levels were also associated with increasing PSA levels and Gleason scores. Particularly, the levels of miR-16 (p = 0.002), miR-148a (p = 0.006) and miR-195 (p = 0.006) significantly correlated with high-risk Gleason scores, whereby miR-148a (p = 0.003) was also significantly associated with increasing PSA values. The high miRNA levels before RP remained increased in the postsurgical plasma samples. Our findings show a network of deregulated plasma miRNAs. In particular, miR-16, miR-148a and miR-195 are involved in the regulation of the PI3K/Akt signaling pathway. These miRNAs may be promising therapeutic targets for high-risk PCa stratification.
“…It would be interesting to explore whether miR-148a could, by modulating (P)RR-mediated functions, such as regulating lipid metabolism, maintaining V-ATPase integrity, and activating tissue RAS, contribute to the onset and progression of such diseases. Among these diseases, it is interesting to notice that plasma miR-148a levels were elevated in LN patients and mice [37]. LN patients have higher risks for ischemic heart diseases [38,39], which is likely caused by abnormalities in lipid metabolism.…”
3'-UTR, 3' untranslated region; DN, diabetic nephropathy; IDOL, inducible degrader of LDLR; NS, nephrotic syndrome; PCSK9, protein convertase subtilisin/kexin9; (P)RR, (pro)renin receptor; RAS, renin-angiotensin system; SORT1, sortilin-1.
Abstract:High plasma LDL cholesterol (LDL-c) concentration is a major risk factor for atherosclerosis. Hepatic LDLR regulates LDL metabolism, and thereby plasma LDL-c concentration. Recently, we identified the (pro)renin receptor [(P)RR] as a novel regulator of LDL metabolism, which regulates LDLR degradation and hence its protein abundance and activity. In silicon analysis suggests that the (P)RR is a target of miR-148a. In this study we determined whether miR-148a could regulate LDL metabolism by regulating (P)RR expression in HepG2 and Huh7 cells. We found that miR-148a suppressed (P)RR expression by binding to the 3'-untranslated regions (3'-UTR) of (P)RR mRNA. Mutating the binding sites for miR-148a in the 3'-UTR of (P)RR mRNA abolished the inhibitory effects of miR-148a on (P)RR expression. In line with our recent findings, reduced (P)RR expression resulted in decreased cellular LDL uptake, likely as a consequence of decreased LDLR protein abundance. Overexpressing the (P)RR prevented miR-148ainduced reduction in LDLR abundance and cellular LDL uptake. Our study supports a new concept that miR-148a is a regulator of (P)RR expression. By reducing (P)RR abundance, miR-148a decreases LDLR protein abundance and consequently cellular LDL uptake.
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