miR‐124 promotes neural differentiation in mouse bulge stem cells by repressing Ptbp1 and Sox9

Abstract

Hair follicle stem cells (HFSCs) are able to differentiate into neurons and glial cells. Distinct microRNAs (miRNAs) regulate the proliferation and differentiation of HFSCs. However, the exact role of miR‐124 in the neural differentiation of HFSCs has not been elucidated. HFSCs were isolated from mouse whisker follicles. miR‐9, let‐7b, and miR‐124, Ptbp1 , and Sox9 expression levels were detected by real‐time polymerase chain reaction (RT‐PCR). The influence of miR‐124 transfection was evaluated using immunostaining. We demonstrated that miR‐124 and let‐7b expression levels were significantly increased after the neural differentiation. Sox9 and Ptbp1 were identified as the target of miR‐124 in the HFSCs. During neural differentiation and miR‐124 mimicking, Ptbp1 and Sox9 levels were decreased. Moreover, the miR‐124 overexpression increased MAP2 (58.43 ± 11.26) and NeuN (48.34 ± 11.15) proteins expression. The results demonstrated that miR‐124 may promote the differentiation of HFSCs into neuronal cells by targeting Sox9 and Ptbp1.

Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder

“…The discrepancy could be due to the fact that other key transcription factors were not transfected along with miR-124 as published previously [20]. Further, most of the studies using miR-124 were conducted either in cellular models derived from mouse [21, 22] or in humans derived from stem cells originating from different cellular origins such as mesenchymal stem cells [23]. Finally, we performed a comprehensive bioinformatic analysis to reveal gene targets that can help us identify the biological function and the relationship of miR-1290 to ASD.…”

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

“…Among the proteins that decrease with age, Hnrnp [110], Ddx5 [111], Ilf3 [112], Lamtor5 [113], Psmd2 [114], Ddb1 [115], Psmd6 [116], and Chd4 [117] are reported to related to cell proliferation and differentiation. Ptbp1 [118], Chd4 [119], Ruvbl2 [120], Cul4a [121], and Lama4 [122] are required for early developmental processes and neuronal differentiation, and some proteins also presented in the inner ear, such as P3h1 [123], Sorcs2 [78], Panx3 [124,125], Idh1 [126], Lamb1 [127]. P3h1 knockout mice showed dysplasia of middle ear bones and hearing impairment [123].…”

The Roles of Non-coding RNA in the Development and Regeneration of Hair Follicles: Current Status and Further Perspectives

“…In vitro miRNA-214 overexpression represses HFSC proliferation and differentiation, alters the cell cycle by directly targeting enhancer of zeste homolog 2 and disrupts Wnt/β-catenin signalling; however, these findings have yet to be validated in vivo ( Du et al, 2018 ). Interestingly, a positive miRNA regulator of HFSCs has also been discovered; miRNA-124 was shown to promote the differentiation of HFSCs by targeting Sox9 and polypyrimidine tract binding protein 1 ( Mokabber et al, 2019 ).…”