Malassezia cuniculisp. nov., a novel yeast species isolated from rabbit skin

Abstract

Members of the genus Malassezia have rarely been associated with lagomorphs. During the course of an investigation of the lipophilic mycobiota of rabbit skin, two lipiddependent isolates which could not be identifi ed were recovered on Leeming and Notman agar medium from different animals. No growth of Malassezia yeasts was obtained either on Sabouraud ' s glucose agar or modifi ed Dixon agar media. In this study, we describe a new taxon, Malassezia cuniculi sp. nov., including its morphological and physiological characteristics. The validation of this new species was supported by analysis of the D1/ D2 regions of the 26S rRNA gene and the ITS-5.8S rRNA gene sequences. The results of these studies confi rm the separation of this new species from the other species of the genus Malassezia , as well as the presence of Malassezia yeasts on lagomorphs. (Table 1) and of these M. caprae , M. equina and M. nana have only been isolated from domestic animals [5].In this paper, we describe a new lipid-dependent species in the genus Malassezia isolated in the course of an investigation of the lipophilic mycobiota of the healthy skin of rabbits. For these isolates we proposed the name Malassezia cuniculi sp. nov. Material and methods Animals, sampling and culture media usedThe study was conducted during 2009 on 11 healthy New Zealand white male rabbits ( Oryctolagus cuniculus ) at the Rabbit Production Unit of the IRTA-Torre Marimon (Caldes de Monbui, Barcelona, Spain) which were used for breeding and meat production.Samples were collected from the external ear canals and the perianal, the inguinal and the submandibular areas of the skin. Perianal skin areas and right and left external ear canals of each animal were sampled by using a swab soaked in the wash fl uid, i.e., 0.075 mol/l phosphate-buffered physiological saline, pH 7.9 containing 0.1% Tween 80. Areas of the chin and groin were sampled using a modifi cation of the detergent scrub technique [6,7]. Skin areas were gently rubbed for 20 seconds with sterile tubes containing 2 ml of wash fl uid. Aliquots of 25 μ l of centrifuged wash fl uid (4000 rpm, 10 min) were inoculated onto paired quadrants of duplicate agar plates. Med Mycol Downloaded from informahealthcare.com by University of Nebraska on 01/05/15 For personal use only. © 2011 ISHAM, Medical Mycology, 49, 40-48 Malassezia cuniculi sp. nov.

Psoriasis and Malassezia Yeasts

“…globosa, M. obtusa and M. restricta (Gueho et al, 1996). M. pachydermatis is the only non lipid-depended species confirmed to be associated with animals, while remaining species are obligatory lipophilic and found primarily in humans (Guillot & Bond, 1999 (Sugita et al, 2002;Sugita et al, 2003;Sugita et al, 2004;Hirai et al, 2004;Cabañes et al, 2007;Cabañes et al, 2011). At present, 14 species of Malassezia have been identified.…”

Atividade antifúngica do óleo essencial de Origanum vulgare frente a Malassezia pachydermatis

“…O gênero Malassezia possui 14 espécies, todas lipodependentes (Cabañes et al, 2011), com exceção da M. pachydermatis, única não-lipodependente e a mais envolvida na casuística dermatológica da clínica de pequenos animais (Campbell et al, 2010). Alterações no microambiente do conduto auditivo, como aumento da temperatura, umidade e substrato, favorecem o aumento do número de células e a passagem da levedura da forma comensal para a parasitária ou patogênica.…”

Prevalence of Malassezia species in patients with pityriasis versicolor in Rosario, Argentina

“…nov., have only been isolated from domestic animals. 4 There is little information about the epidemiology and ecology of Malassezia species; hence, the clinical significance of these species is not fully distinguished. Some species are polymorphic, in particular M. furfur; others have fastidious requirements for growth, and their maintenance in subculture is difficult, such as M. globosa and M. restricta.…”

“…However, they may become pathogenic under certain conditions and are associated with a broad spectrum of clinical infections as pityriasis versicolor (PV), folliculitis, seborrheic dermatitis, some forms of atopic dermatitis, confluent and reticulate papillomatosis, and even systemic infections. 4,7,23,28,30,35 PV is common in late teens and young adults of both sexes, and is characterized by well-demarcated scaling patches with variable pigmentation. 12 Although PV was described at the beginning of the nineteenth century; its etiological agents have been a matter of debate.…”

“…13 This genus includes the following fourteen species: Malassezia pachydermatis, Malassezia furfur, Malassezia sympodialis, Malassezia globosa, Malassezia obtusa, Malassezia restricta, Malassezia slooffiae, Malassezia caprae, 3,14,15,17,18,29,39 Malassezia dermatis, 41 Malassezia japonica, 42 Malassezia equina, 3 Malassezia nana, 24 Malassezia yamatoensis 43 and Malassezia cuniculi sp. nov. 4 M. nana, M. equina, M. caprae and Malassezia cuniculi sp. nov., have only been isolated from domestic animals.…”