<i>M. tuberculosis</i>in Lymph Node Biopsy Paraffin-Embedded Sections

Eshete, Abdurehman; Zeyinudin, Ahmed; Ali, Solomon; Abera, Solomon; Mohammed, Abdurehman Eshete

doi:10.1155/2011/127817

Cited by 18 publications

(15 citation statements)

References 14 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Available evidence reveals TB lymphadenitis to occur relatively early, after M. tuberculosis primary infection, frequently affecting young people in endemic countries. 20 The female-to-male ratio of 1 to 1 obtained in this study is indicative of TB infection to be related to exposure rather than gender (Table 1).…”

Section: Discussionmentioning

confidence: 61%

“…Of the 160 FFPE tissue specimens tested, none were AFB ZN positive contrary to the previously reported 61.7% AFB positive. 20 Our retrospective study used laboratory microscopic findings that are generally nonspecific with other conditions, such as sarcoidosis, syphilis, leprosy, Crohn's disease, rheumatoid arthritis, systemic lupus erythematous, and pneumoconiosis, having similar features. 23 The failure of ZN to detect AFB in FFPE tissue specimen could be a result of paucibacillary nature of the tissue specimen.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Potential Value of Qiagen and PrepIT•MAX Kits in Extraction of Mycobacterial DNA From Presumptive Tuberculosis Archived Formalin-Fixed Paraffin-Embedded Tissues

Ayub

Mollel

Mbugi

2018

EAHRJ

View full text Add to dashboard Cite

Background: DNA analysis has potential for screening for and diagnosing a variety of conditions as well as the characterization of various pathogens for many purposes including to identify genetic disorders and mutations, study genetic diversity, and establish evolutional trends. Methods:Our study compared the performance of 2 DNA extraction kits: Qiagen and prepITMAX. The study tested 160 formalin-fixed paraffin-embedded (FFPE) human tissue samples that had been collected at Muhimbili National Hospital (MNH) between 2010 and 2016. For each sample, DNA extraction was performed using both the Qiagen and prepITMAX kits followed by polymerase chain reaction (PCR) tests to target the RNA polymerase gene and gel electrophoresis.Results: The findings showed that the Qiagen was 3 times superior to the prepITMAX kit in successfully extracting mycobacterial DNA from presumptive tuberculosis (TB) FFPE tissues. Of the 160 previously Ziehl-Neelsen stain-negative Mycobacterium tuberculosis suspicious tissue samples, 12 (7.5%) tested positive with the PCR. Of the 12 PCR-detected positive samples, 8 (66.7%) yielded positive results with the Qiagen kit only and 4 (33.3%) yielded positive results with both Qiagen and prepITMAX kits. Additionally, 10 (83.3%) came from well-formed granuloma, 1 (8%) from caseous necrosis, and 1 (8.3%) Langhans-type giant cells endorsing their potential for housing infection such as TB adenitis.Conclusions: A combination of molecular techniques, microscopy, and pathological features increases detection of M. tuberculosis from FFPE tissues. Both the Qiagen and the prepITMAX DNA extraction kits have shown a remarkable capability for extracting DNA from M. tuberculosis, although examination of FFPE tissues is not an intended use for the prepITMAX, according to the manufacturer. In resource-limited countries, however, these kits may complement each other. We recommend further studies for validation and optimization, which includes the cost effectiveness of prepITMAX extraction kit to advocate for its use in extraction of mycobacterial DNA from FFPE tissues.

show abstract

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Potential Value of Qiagen and PrepIT•MAX Kits in Extraction of Mycobacterial DNA From Presumptive Tuberculosis Archived Formalin-Fixed Paraffin-Embedded Tissues

Ayub

Mollel

Mbugi

2018

EAHRJ

View full text Add to dashboard Cite

show abstract

“…Whether it is lymph node tuberculosis or histiocytic necrotizing lymphadenitis, histopathological examination is the gold standard for diagnosis [19,20]. The characteristic manifestation of lymph node tuberculosis is caseous necrosis, which appears through red-stained granularity in HE stains, with neutrophil infiltration; surrounded by granuloma formation and by multinuclear giant cell reactions.…”

Section: Discussionmentioning

confidence: 99%

Comparison of clinical and pathological features of lymph node tuberculosis and histiocytic necrotizing lymphadenitis

Yang

2019

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The aim of this study was to investigate the basis for a differential diagnosis of lymph node tuberculosis and histiocytic necrotizing lymphadenitis. Methodology: This study selected 85 cases of lymph node tuberculosis (Group A patients) and 26 cases of histiocytic necrotizing lymphadenitis (Group B patients). The clinical and pathology features on both groups were analysed. Results: The Group A patients were older than the Group B patients (t = 5.233, P < 0.01); The Group B patients had less tuberculosis exposure history (x2 = 4.279, P < 0.01), and a higher frequency of tenderness (χ2 = 8.109, P < 0.01) and fever (x2 = 31.923, P < 0.01). The Group A patient group had a higher WBC level (t = 2.980, P < 0.01) and lower serum ALB (t = 5.508, P < 0.01); As seen through ultrasound imaging, Group B patients had more clear boundaries (70.59%), higher low-echo rates (82.36%) and low calcification rates (0%), Group A patients for whom these rates was 25.76%, 40.91% and 25.76% respectively. In terms of pathology data, the main manifestations of Group A patients were granulomatous inflammation with caseous necrosis, multinuclear giant cell reaction, and in some cases, acid-fast bacilli smears (+). In Group B patients, there were instances of coagulative necrosis surrounded by foam-like tissue cells without neutrophil infiltration. Conclusion: We found that the epidemiological history, clinical symptoms, laboratory examinations, ultrasound imaging and changes in pathology are very important for the identification of lymph node tuberculosis and histiocytic necrotizing lymphadenitis.

show abstract

“…Study conducted by Greenwood and Fox in 1973 shows 33 (47.1%) positive for AFB on ZN staining technique out of 70 tuberculosis histology cases [15]. Study conducted by Eshete and others in 2011 shows 37 (61.7%) positive for AFB after ZN staining technique in 60 lymph nodes with tuberculous histology [16]. Study conducted by Rasool et al, in 2017 shows 52.58% positive for AFB after routine ZN stain [17].…”

Section: Discussionmentioning

confidence: 99%

Detection of Acid Fast Bacilli in Tuberculous Lymph Node Tissue and Touch Preparations—A Comparative Study

Rasool¹,

Siraj²,

Naseem³

et al. 2017

JTR

View full text Add to dashboard Cite

Background: The most common form of extrapulmonary tuberculosis is lymphadenitis. Lymphadenitis is considered mainly a disease of young children and has a peak age of onset at 20 to 40 years. Objectives: The purpose of this study was to detect and compare the presence of acid fast bacilli in lymph node biopsies and their touch preparations using Ziehl Neelsen and fluorescence staining. Materials and Methods: The lymph node biopsies and their touch preparations were prepared and stained with Haematoxylin and eosin to determine their morphology. Slides were stained with ZN staining and fluorescent staining technique using Auramine O and Rohdamine B to demonstrate the presence of acid fast bacilli in tissue and their touch preparations. Results: AFB was detected in 56% of biopsies and 54% of touch preparations. In addition in these cases we observed that fluorescent staining technique using Auramine O and Rohdamine B is superior to ZN stain because low magnification used in fluorescent staining technique makes it possible to scan the smear rapidly and detects the AFB as glowing spots even if it is present in small numbers. Conclusion: The results of AFB in tissue biopsies are in equivalent in their touch preparation.

show abstract

M. tuberculosisin Lymph Node Biopsy Paraffin-Embedded Sections

Cited by 18 publications

References 14 publications

Potential Value of Qiagen and PrepIT•MAX Kits in Extraction of Mycobacterial DNA From Presumptive Tuberculosis Archived Formalin-Fixed Paraffin-Embedded Tissues

Potential Value of Qiagen and PrepIT•MAX Kits in Extraction of Mycobacterial DNA From Presumptive Tuberculosis Archived Formalin-Fixed Paraffin-Embedded Tissues

Comparison of clinical and pathological features of lymph node tuberculosis and histiocytic necrotizing lymphadenitis

Detection of Acid Fast Bacilli in Tuberculous Lymph Node Tissue and Touch Preparations—A Comparative Study

Contact Info

Product

Resources

About