<i>Listeria</i>
            Placental Infection

Vázquez‐Boland, José A.; Krypotou, Emilia; Scortti, Mariela

doi:10.1128/mbio.00949-17

Cited by 59 publications

(46 citation statements)

References 39 publications

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“…The Gram-positive, facultative intracellular bacterium Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne infection with a high mortality rate ( 1 ). This pathogen is ubiquitous, and it has been isolated from humans and animals, as well as from raw and ready-to-eat foods ( 2 , 3 ), and it is capable of invading a wide variety of eukaryotic cells, including endothelial cells and macrophages ( 4 , 5 ).…”

Section: Introductionmentioning

confidence: 99%

Carboxyl-Terminal Residues N478 and V479 Required for the Cytolytic Activity of Listeriolysin O Play a Critical Role in Listeria monocytogenes Pathogenicity

Cui

Jiang

et al. 2017

Front. Immunol.

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Listeria monocytogenes is a facultative intracellular pathogen that secretes the cytolysin listeriolysin O (LLO), which enables the bacteria to cross the phagosomal membrane. L. monocytogenes regulates LLO activity in the phagosome and minimizes its activity in the host cytosol. Mutants that fail to compartmentalize LLO activity are cytotoxic and have attenuated virulence. Here, we showed that residues N478 and V479 of LLO are required for LLO hemolytic activity and bacterial virulence. A single N478A mutation (LLON478A) significantly increased the hemolytic activity of LLO at a neutral pH, while no difference was observed at the optimum acidic pH, compared with wild-type LLO. Conversely, the mutant LLOV479A exhibited lower hemolytic activity at the acidic pH, but not at the neutral pH. The double mutant LLON478AV479A showed a greater decrease in hemolytic activity at both the acidic and neutral pHs. Interestingly, strains producing LLON478A or LLOV479A lysed erythrocytes similarly to the wild-type strain. Surprisingly, bacteria-secreting LLON478AV479A had barely detectable hemolytic activity, but exhibited host cell cytotoxicity, escaped from the phagosome, grew intracellularly, and spread cell-to-cell with the same efficiency as the wild-type strain, but were highly attenuated in virulence in mice. These data demonstrate that these two residues are required for LLO hemolytic activity and pathogenicity in mice, but not for escape from the phagosome and cell-to-cell spreading. The finding that the nearly non-hemolytic LLON478AV479A mutant grew intracellularly indicates that mutagenesis of a virulence determinant is a novel approach for the development of live vaccine strains.

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Section: Introductionmentioning

confidence: 99%

Carboxyl-Terminal Residues N478 and V479 Required for the Cytolytic Activity of Listeriolysin O Play a Critical Role in Listeria monocytogenes Pathogenicity

Cui

Jiang

et al. 2017

Front. Immunol.

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“…Vibrio parahaemolyticus and Listeria monocytogenes are important facultative and food-borne pathogens in the food industry Vazquez-Boland et al, 2017;Chimalapati et al, 2018). In 2014, 605 cases of V. parahaemolyticus infections in the United States were reported according to the Cholera and Other Vibrio Illness Surveillance (Centers for Disease Control and Prevention [CDC], 2014).…”

Section: Introductionmentioning

confidence: 99%

Stress Response of Vibrio parahaemolyticus and Listeria monocytogenes Biofilms to Different Modified Atmospheres

Qian

Guo

et al. 2020

Front. Microbiol.

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The sessile biofilms of Vibrio parahaemolyticus and Listeria monocytogenes have increasingly become a critical threat in seafood safety. This study aimed to evaluate the effects of modified atmospheres on the formation ability of V. parahaemolyticus and L. monocytogenes biofilms. The stress responses of bacterial biofilm formation to modified atmospheres including anaerobiosis (20% carbon dioxide, 80% nitrogen), micro-aerobiosis (20% oxygen, 80% nitrogen), and aerobiosis (60% oxygen, 40% nitrogen) were illuminated by determining the live cells, chemical composition analysis, textural parameter changes, expression of regulatory genes, etc. Results showed that the biofilm formation ability of V. parahaemolyticus was efficiently decreased, supported by the fact that the modified atmospheres significantly reduced the key chemical composition [extracellular DNA (eDNA) and extracellular proteins] of the extracellular polymeric substance (EPS) and negatively altered the textural parameters (biovolume, thickness, and bio-roughness) of biofilms during the physiological conversion from anaerobiosis to aerobiosis, while the modified atmosphere treatment increased the key chemical composition of EPS and the textural parameters of L. monocytogenes biofilms from anaerobiosis to aerobiosis. Meanwhile, the expression of biofilm formation genes (luxS, aphA, mshA, oxyR, and opaR), EPS production genes (cpsA, cpsC, and cpsR), and virulence genes (vopS, vopD1, vcrD1, vopP2β, and vcrD2β) of V. parahaemolyticus was downregulated. For the L. monocytogenes cells, the expression of biofilm formation genes (flgA, flgU, and degU), EPS production genes (Imo2554, Imo2504, inlA, rmlB), and virulence genes (vopS, vopD1, vcrD1, vopP2β, and vcrD2β) was upregulated during the physiological conversion. All these results indicated that the modified atmospheres possessed significantly different regulation on the biofilm formation of Gram-negative V. parahaemolyticus and Gram-positive L. monocytogenes, which will provide a novel insight to unlock the efficient control of Gram-negative and Gram-positive bacteria in modified-atmosphere packaged food.

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“…In the high-risk group, pregnant women are at the highest risk of contracting listeriosis, which can potentially lead to eISSN: 2550-2166 © 2019 The Authors. Published by Rynnye Lyan Resources miscarriage, stillbirth, preterm birth, and congenital neonatal infections (Vázquez-boland et al, 2017). Nayak et al (2018) reported that a three-year-old child developed infection due to L. monocytogenes and was treated with IV ceftriaxone.…”

Section: Introductionmentioning

confidence: 99%

A review on Listeria monocytogenes in food: prevalence, pathogenicity, survivability and antibiotic resistance

Wai¹,

Tang²,

New³

et al. 2019

Food Res.

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The ubiquitous of Listeria monocytogenes in the environment and in the niche of foods can be related with the ability of the pathogen to survive in adverse conditions, in example, low temperature and pH, high concentrations of salt and bile, oxidative stress and carbon starvation. Besides, most of the human listeriosis are food-borne and L. monocytogenes consists of a spectrum of strains with varying virulence and pathogenicity. Moreover, the virulent regulators play a role in facilitating better adaption or persistence of L. monocytogenes in the food environment. This review included prevalence, pathogenicity, survivability and antibiotic resistance of L. monocytogenes in various categories of foods such as meat, seafood, dairy products and vegetables. The recent updates on listeriosis or foodborne cases associated with L. monocytogenes should be studied in order to have a better understanding of their persistence in the food chain and its public health impact especially among the high-risk groups: elderly, infant, pregnant woman and immunocompromised individuals.

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Listeria Placental Infection

Cited by 59 publications

References 39 publications

Carboxyl-Terminal Residues N478 and V479 Required for the Cytolytic Activity of Listeriolysin O Play a Critical Role in Listeria monocytogenes Pathogenicity

Carboxyl-Terminal Residues N478 and V479 Required for the Cytolytic Activity of Listeriolysin O Play a Critical Role in Listeria monocytogenes Pathogenicity

Stress Response of Vibrio parahaemolyticus and Listeria monocytogenes Biofilms to Different Modified Atmospheres

A review on Listeria monocytogenes in food: prevalence, pathogenicity, survivability and antibiotic resistance

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