<i>Leptospira</i> spp. in Naturally Infected Dairy Cow from a Brazilian Border Region

Gonçalves, Daniela Dib; Pastre, Giovani Batista; Rey, Laisa Marina Rosa; Fazoli, Kawany Gabrieli Zanetti; Silva, Lucas Lima da; Ferreira, Larissa Rafaela de Paula; Fritzen, Juliana Torres Tomazi; Chideroli, Roberta Torres; Durel, Luc; Decuadro-Hansen, G.; Lourenço, Emerson Luiz Botelho; Junior, Ranulfo Piau; Barbosa, Lidiane Nunes; Pereira, Ulisses de Pádua; Santos, Isabela Carvalho dos

doi:10.1089/vbz.2021.0040

Cited by 2 publications

(3 citation statements)

References 24 publications

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“…To our knowledge, only one previous study combined herd serological screening and PCR of genital samples to diagnose leptospirosis in cows with a history of reproductive problems, with significant results. 11 Although extremely important, that study tested vaginal samples instead of CVM, and urinary contamination of the samples cannot be discarded. To truly demonstrate genital colonisation, it has been recommended that CVM samples should be collected in the vaginal fornix to avoid urine contamination.…”

Section: Discussionmentioning

confidence: 99%

“…reinforced the association of gestational losses in the first trimester of gestation with leptospirosis, they only performed the serological screening of the herds, which does not confirm genital colonisation. The microscopic agglutination test (MAT) is an indirect method that is reliable at the herd level, 8 while CVM PCR has been used in studies involving slaughtered sheep 9 and in the individual diagnosis of cows 10,11 . Until now, only one study used the approach of herd serology combined with individual PCR of cows with reproductive disorders 11 ; however, the genital sample used was vaginal fluid and not CVM, which may not be free of urine contamination of the sample.…”

Section: Introductionmentioning

confidence: 99%

“…The microscopic agglutination test (MAT) is an indirect method that is reliable at the herd level, 8 while CVM PCR has been used in studies involving slaughtered sheep 9 and in the individual diagnosis of cows. 10,11 Until now, only one study used the approach of herd serology combined with individual PCR of cows with reproductive disorders 11 ; however, the genital sample used was vaginal fluid and not CVM, which may not be free of urine contamination of the sample. Thus, the two-step BGL diagnosis approach of herd serological screening (MAT) followed by confirmatory individual CVM PCR has never been performed in cows under field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Field application of a combined serological‐molecular testing protocol for diagnosing genital leptospirosis in naturally infected cows with gestational losses

Aymée,

Zambrano,

Escalona

et al. 2023

Veterinary Record

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BackgroundBovine genital leptospirosis (BGL) causes chronic reproductive disease in cattle. This study aimed to apply a combined serological‐molecular testing protocol under field conditions for diagnosing BGL in cows with gestational losses.MethodsThree beef herds with reproductive failures were studied, and 60 cows with gestational losses (20 from each herd) were randomly selected for laboratory diagnosis of BGL. In addition, 40 cows with normal pregnancy were included as a control. Blood samples were collected from all 100 cows for microscopic agglutination testing, and cervicovaginal mucus (CVM) samples were collected from 28 cows with gestational losses and 20 control cows for lipL32‐PCR.ResultsAll herds had high Leptospira seroreactivity (>65%), mainly against serogroup Sejroe. Ten of the 28 CVM samples from cows with gestational losses were PCR‐positive, while all samples from the control group were negative (p < 0.05).LimitationsUnfortunately, the positive samples did not amplify in secY‐PCR for nucleotide sequencing, which would allow the identification of leptospiral strains.ConclusionSerology was sufficient to indicate leptospirosis at the herd level, but the definitive diagnosis of BGL was only possible using CVM PCR. Although seroreactivity against serogroup Sejroe has been associated with gestational losses, this is the first study to conduct CVM PCR as a confirmatory test for BGL diagnosis in extensive beef herds under field conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Field application of a combined serological‐molecular testing protocol for diagnosing genital leptospirosis in naturally infected cows with gestational losses

Aymée,

Zambrano,

Escalona

et al. 2023

Veterinary Record

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Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil

Candeias,

Snak,

Pretto-Giordano

et al. 2023

Sem. Ci. Agr.

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Leptospirosis is caused by spirochete bacteria of the genus Leptospira and is considered the most widespread zoonosis worldwide. It is an important agent that causes animal production to decrease. In cattle, it affects especially the reproductive tract. The objective of this study was to determine the seroprevalence of Leptospira spp., molecularly detect the bacteria in tissues of aborted fetuses, and identify the main risk factors associated with infection in cattle in dairy farms in Western Paraná. For this purpose, 600 bovine serum samples from 60 properties and 17 bovine fetuses from nine properties were collected. Data about the properties were also collected through an epidemiological questionnaire to assess the main risk factors associated with Leptospira spp. infection. The serum samples were analyzed using microscopic agglutination test (MAT), and the fetal tissues using nested polymerase chain reaction (nested PCR). Seroprevalence of Leptospira spp. in dairy cattle in Western Paraná was 39.83% (239/600) and none of the analyzed fetuses were positive for Leptospira spp. The main risk factors identified are related to the production system, reproductive management, and the presence of dogs on the property. Leptospira spp. infection is widely spread in the cattle population in Western Paraná.

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Leptospira spp. in Naturally Infected Dairy Cow from a Brazilian Border Region

Cited by 2 publications

References 24 publications

Field application of a combined serological‐molecular testing protocol for diagnosing genital leptospirosis in naturally infected cows with gestational losses

Field application of a combined serological‐molecular testing protocol for diagnosing genital leptospirosis in naturally infected cows with gestational losses

Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil

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