Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice

Abstract

Objectives Hyperinsulinemia increases the risk factor of diabetes and infertility at a manifold. Lactobacillus plantarum has several medical significances with limited reports. Hence, this study assessed the effect of L. plantarum on sexual-reproductive functions and distribution of insulin receptors in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice. Methods Forty male adult mice were divided into five groups as follows: control, high-fat diet (HFD) + streptozotocin (STZ), therapeutic, co-administration group type 1 (CO-AD) and probiotics. They were either simultaneously exposed to an HFD and L. plantarum treatment for 28 days with a dose of STZ injection to induce hyperinsulinemia on day 28 or treated with L. plantarum for 14 days, and following induction of hyperinsulinemia. Mice were subjected to a sexual behavioural test and thereafter sacrificed under euthanasia condition. Blood, brain and testes were collected for biochemical and immunohistochemical assays. Results Treatment with L. plantarum ameliorated reproductive hormones activity disruption, sexual behavioural defects, antioxidant imbalance, insulin dysregulation and lipid metabolism dysfunction following exposure to HFD + STZ when compared to the hyperinsulinemic untreated mice. Conclusions Taken together, data from this study reveal that L. plantarum abrogated hyperinsulinemia-induced male sexual and reproductive deficits by modulating antioxidant status, lipid metabolism and insulin signalling in the hypothalamic-pituitary-testicular axis of mice.

Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

“…25,26,[87][88][89] Intriguingly, the oral administration of L. plantarum restored male fertility in diabetic mice and rats, in mice exposed to DEHP, as well as in rats exposed to DBP or MCH. 25,26,[87][88][89] There are several possible explanations for this: L. plantarum may decrease inflammation, alleviate oxidative stress, induce anti-apoptotic events, absorb DBP at neutral pH, and bind to heavy metals. 25,26,[87][88][89] To the best of our knowledge, this is the first report studying the impact of L. plantarum secretions on human semen cryopreservation by taking sperm motility, morphology, and DNA fragmentation into consideration.…”

“…Prominently, L. plantarum has been found to be an effective treatment for male infertility in animal studies 25,26,87–89 . Among others, obesity, diabetes, and exposure to toxic molecules (e.g., diethylexylphtalate [DEHP], di‐n‐butyl phthalate [DBP], mercuric chloride [MCH]) have previously been shown to affect the male reproductive system in animals 25,26,87–89 . Intriguingly, the oral administration of L. plantarum restored male fertility in diabetic mice and rats, in mice exposed to DEHP, as well as in rats exposed to DBP or MCH 25,26,87–89 .…”

“…25,26,[87][88][89] There are several possible explanations for this: L. plantarum may decrease inflammation, alleviate oxidative stress, induce anti-apoptotic events, absorb DBP at neutral pH, and bind to heavy metals. 25,26,[87][88][89] To the best of our knowledge, this is the first report studying the impact of L. plantarum secretions on human semen cryopreservation by taking sperm motility, morphology, and DNA fragmentation into consideration. However, more experiments analyzing the impact of L.…”

“…25,26,[87][88][89] have previously been shown to affect the male reproductive system in animals. 25,26,[87][88][89] Intriguingly, the oral administration of L. plantarum restored male fertility in diabetic mice and rats, in mice exposed to DEHP, as well as in rats exposed to DBP or MCH. 25,26,[87][88][89] There are several possible explanations for this: L. plantarum may decrease inflammation, alleviate oxidative stress, induce anti-apoptotic events, absorb DBP at neutral pH, and bind to heavy metals.…”

Naturally Based Nano Formulation in Metabolic and Reproductive Disorders: A Review

“…Insulin plays a crucial role in the hypothalamicpituitary-gonadal axis so insulin insufficiency leads to hypogonadotropic and hypogonadism. Insulin promotes gonadotropin-releasing hormone (GnRH) release in cell lines obtained from hypothalamus cells (Edem et al 2021).…”