Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice

Abstract

Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to analyze the serum and tissues of mice with oxidative aging induced by d-galactose, which showed that KSFY06 can inhibit the decline of heart, liver, spleen, and kidney caused by aging. The KSFY06 strain increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione (GSH) in serum and liver of aging mice, while the content of malondialdehyde (MDA) is reduced. Pathological observation showed that KSFY06 alleviated damage to the liver, spleen, and skin of oxidative aging mice. qPCR showed that, at high dose (2 × 10 9 cfu/kg per day), KSFY06 upregulates copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), catalase (CAT) mRNA expression, and its downstream inducible nitric oxide synthase (iNOS) mRNA expression in liver and spleen tissues induced by d-gal. To a certain extent, these findings indicate that L. plantarum KSFY06 is able to protect against oxidative stress in the dgal-induced aging model. In conclusion, L. plantarum KSFY06 may provide a potential research value in the prevention or alleviation of related diseases caused by oxidative stress. K E Y W O R D Saging, Lactobacillus plantarum KSFY06, mice, oxidation

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

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Effects of Cold-Pressing and Hydrodistillation on the Active Non-volatile Components in Lemon Essential Oil and the Effects of the Resulting Oils on Aging-Related Oxidative Stress in Mice

“…Overall, the effects of plant essential oils on free radicals and damaged tissues result in inhibition or delay of aging. SOD, T-AOC, GSH, and GSH-Px are key indicators of oxidation (25,26). The levels of oxidative stress can be determined through analysis of human or animal serum.…”

“…SOD, T-AOC, GSH, and GSH-Px are key indicators of oxidation ( 25 , 26 ). The levels of oxidative stress can be determined through analysis of human or animal serum.…”

Ganoderma lucidum Rhodiola compound preparation prevent d-galactose-induced immune impairment and oxidative stress in aging rat model

“…Besides, during the test of using d-gal, the experimental animal has a high survival rate. So, d-gal has been widely used in anti-aging pharmacological research 22 . In this experiment, we successfully induced the aging rats model by using d-gal, and no rat died during the modeling process.…”