<i>Lactobacillus plantarum</i> HNU082 alleviates dextran sulfate sodium-induced ulcerative colitis in mice through regulating gut microbiome

Wu, Yuqing; Li, Ao; Liu, Huanwei; Zhang, Zeng; Zhang, Chengcheng; Ma, Chenchen; Zhang, Lin; Zhang, Jiachao

doi:10.1039/d2fo02303b

Cited by 28 publications

(21 citation statements)

References 56 publications

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“…A deep Shotgun metagenomic sequence was used to perform microbial community diversity analysis. 40 HumanN2 metagenomic metabolic pathway annotations were constructed based on the UniRef90 database. 41 The R software and GraphPad software were used for statistical analysis.…”

Section: Rna Extraction Pcr Amplification Electrophoresis and 16s Rrn...mentioning

confidence: 99%

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Chen

et al. 2022

Food Funct.

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Section: Rna Extraction Pcr Amplification Electrophoresis and 16s Rrn...mentioning

confidence: 99%

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Chen

et al. 2022

Food Funct.

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“…The relieving effect of L. plantarum on intestinal inflammation has been reported in other studies [ 56 , 57 ]. The gene expression and production of anti-inflammatory factors (IL-10, TGF-β1, and TGF-β2) increased in Caco-2 cells and a DSS-induced mouse model [ 58 , 59 ]. L. plantarum reduced the gene expression and production of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, MPO, and IFN-γ) and suppressed inflammatory responses to normalize the immune barrier [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses

et al. 2023

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Lactobacillus plantarum (L. plantarum) is a probiotic that has emerged as novel therapeutic agents for managing various diseases, such as cancer, atopic dermatitis, inflammatory bowel disease, and infections. In this study, we investigated the potential mechanisms underlying the anticancer effect of the metabolites of L. plantarum. We cultured L. plantarum cells to obtain their metabolites, created several dilutions, and used these solutions to treat human colonic Caco-2 cells. Our results showed a 10% dilution of L. plantarum metabolites decreased cell viability and reduced the expression of autophagy-related proteins. Moreover, we found co-treatment with L. plantarum metabolites and chloroquine, a known autophagy inhibitor, had a synergistic effect on cytotoxicity and downregulation of autophagy-related protein expression. In conclusion, we showed the metabolites from the probiotic, L. plantarum, work synergistically with chloroquine in killing Caco-2 cells and downregulating the expression of autophagy-related proteins, suggesting the involvement of autophagy, rather than apoptosis, in their cytotoxic effect. Hence, this study provides new insights into new therapeutic methods via inhibiting autophagy.

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“…In this study, we found that DSS-induced colitis caused a significant decrease in serum Trp content and increase in Kyn content, which was consistent with previous studies ( 32 – 34 ). The decrease in serum Trp content may be due to reduced food intake (the main source of Trp) in colitis mice ( 35 ). The elevation of Kyn content was mainly attributed to the decrease of its consumption.…”

Section: Discussionmentioning

confidence: 99%

DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota

Zhao

Quan

et al. 2023

Front. Immunol.

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Accumulative studies suggest that inflammatory bowel disease (IBD) may cause multiple central nervous system (CNS) pathologies. Studies have found that indoleamine-2,3-dioxygenase (IDO, rate-limiting enzyme of the kynurenine (Kyn) pathway) deficient mice were protected from endotoxin induced cognitive impairment, and Kyn administration induced cognitive memory deficits in both control and IDO-deficient mice. However, there is no investigation of the brain Kyn pathway in IBD, thus we investigated whether dextran sulfate sodium (DSS)-induced colitis could cause dysregulation of Kyn pathway in brain, and also in serum. C57BL/6J mice were given drinking water with 2% DSS for 10 consecutive days to induce colitis. In serum, we found significant increase in Kyn and kynurenic acid (Kyna) level, which was regulated by IDO-1 and KAT2 (rate-limiting enzymes of Trp-Kyn-Kyna pathway). Similarly, by analyzing GEO datasets, higher IDO-1 levels in peripheral blood monocytes and colon of UC patients was found. Furthermore, the Kyn pathway was significantly upregulated in the cerebral cortex under the action of IDO-1 after DSS treatment, which ultimately induced the neurotoxic phenotype of astrocytes. To investigate whether gut microbiota is involved in IBD-induced Kyn pathway dysregulation, we performed intestinal flora 16S rRNA sequencing and found that DSS-induced colitis significantly altered the composition and diversity of the gut microbiota. Metabolic function analysis also showed that Tryptophan metabolism, NOD-like receptor signaling pathway and MAPK signaling pathway were significantly up-regulated in the 2% DSS group. A significant association between intestinal flora and Trp metabolism (both in serum and brain) was found by correlation analysis. Overall, this study revealed that DSS-induced colitis causes dysregulation of the Kyn pathway in serum and brain by affecting rate-limiting enzymes and intestinal flora.

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Lactobacillus plantarum HNU082 alleviates dextran sulfate sodium-induced ulcerative colitis in mice through regulating gut microbiome

Abstract: Probiotic Lp082 treats ulcerative colitis by optimizing the gut microbiota and the intestinal mucosal barrier (biological, chemical, mechanical and immune barriers), regulating inflammatory and disease pathways and reducing neutrophil infiltration.

Cited by 28 publications

References 56 publications

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses

DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota

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