<i>Lactobacillus fermentum</i>Administration Modulates Cytokine Expression and Lymphocyte Subpopulation Levels in Broiler Chickens Challenged with<i>Campylobacter coli</i>

Šefcová, Miroslava Anna; Larrea-Álvarez, Marco; Larrea-Álvarez, César Marcelo; Karaffová, Viera; Révajová, Viera; Gancarčíková, Soňa; Ševčíková, Zuzana; Herich, R.

doi:10.1089/fpd.2019.2739

Cited by 15 publications

(24 citation statements)

References 39 publications

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“…Lactobacilli synbiotics, composed of L. salivarius and galactooligosaccharides, were shown to induce a down-regulatory pattern over cytokine expression (IL-12, IL-8, IL-1β), while levels of IL-18 were in fact upregulated by such treatment [ 40 ]. In the present research, expression levels of IL-8 were not significantly modified by any of the treatments, which differ from previous findings, indicating a decrease in mRNA abundance after L. fermentum administration [ 20 ]. On the other hand, these outcomes agree with previous investigations revealing that C. jejuni administration could not modify IL-18 levels [ 6 ].…”

Section: Discussioncontrasting

confidence: 99%

“…These outcomes corroborate previous findings showing that C. jejuni infection increases IL-15 expression, which has been linked to cellular necrosis and pro-inflammatory responses [ 38 ]. Similarly, probiotic treatments, making use of L. fermentum , have shown to induce an overall reduction of IL-15 abundance [ 20 ]. Conclusively, the probiotic treatment seemed to be quite effective at downregulating IL-15 expression, which could be otherwise increased by C. jejuni especially at 12 hpi.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 108 broiler cock chickens (COBB 500) were used in the experiment, they had constant access to water and feed (free of probiotics, antibiotics, or coccidiostats) ad libitum. Individuals (27 per treatment) were allocated in four groups, following an experimental design used in previous research [ 20 ]. (i) the control group, in which neither of the bacteria were used, (ii) the probiotic group ( L. fermentum , LB), (iii) the Campylobacter -challenged group (Cj), and (iv) the co-exposure group (LBCj).…”

Section: Methodsmentioning

confidence: 99%

“…L. fermentum Biocenol CCM 7514 was cultivated exactly as hitherto described [ 20 ]. C. jejuni CCM 6189 was grown in blood-free selective agar base CM0935 supplemented with SR0155 (containing Cefoperazone and Amphotericin B; Oxoid Ltd., United Kingdom) in a jar with microaerobic conditions (5% O 2 , 10% CO 2 , 85% N 2 , CampyGen; Oxoid Ltd.).…”

Section: Methodsmentioning

confidence: 99%

“…Various strains of L. fermentum have been regarded as promising probiotics, and have been suggested as potential tools for fighting C. jejuni infections, although these results come mainly from in vitro experiments [ 22 , 23 ]. Recently, we demonstrated that treatment with L. fermentum Biocenol CCM 7514 in chickens infected with C. coli promoted a heightened humoral response while dampening potential inflammation mediated by effector T cells in 1-week-old-chickens [ 20 ]. This study also revealed that the downregulation of IL-15 and IL-17, promoted by the probiotic, might be responsible for maintaining intraepithelial lymphocytes in an induced state and avoid their differentiation into effector cells.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Lactobacillus Fermentum Supplementation on Body Weight and Pro-Inflammatory Cytokine Expression in Campylobacter Jejuni-Challenged Chickens

Šefcová

Larrea-Álvarez

et al. 2020

Veterinary Sciences

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Due to the interest in using probiotic bacteria in poultry production, this research was focused on evaluating the effects of Lactobacillus fermentum Biocenol CCM 7514 administration on body weight gain and cytokine gene expression in chickens challenged with Campylobacter jejuni. One-hundred and eight 1-day old COBB 500 broiler chickens were equally assigned to four experimental groups at random. In the control group (C) chicks were left untreated, whereas in groups LB and LBCj a suspension of L. fermentum was administered. A suspension of C. jejuni was subsequently applied to groups Cj and LBCj. Body weight was registered, and the individuals were later slaughtered; cecum samples were collected at 12, 36 and 48 h post-infection (hpi). The entire experiment lasted seven days. Reverse transcription quantitative PCR (RT-qPCR) was used to determine expression levels of IL-1β, IL-15, IL-17, and IL-18 at each time point. Pathogen-infected individuals were observed to weigh significantly less than those fed with the probiotic. Significant differences were also found in transcript abundance; expression of IL-15 was downregulated by the probiotic and upregulated by C. jejuni. The effects of bacterial treatments were time-dependent, as the expression profiles differed at later stages. The present outcomes demonstrate that L. fermentum both reduces the impact of C. jejuni infection on chicken body weight and regulates positively pro-inflammatory cytokine expression, which ultimately increase bird well-being and improves production.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%