<i>Lactobacillus acidophilus</i>stimulates intestinal P-glycoprotein expression via a c-Fos/c-Jun-dependent mechanism in intestinal epithelial cells

Priyamvada, Shubha; Anbazhagan, Arivarasu Natarajan; Kumar, Anoop; Soni, Vikas; Alrefai, Waddah A.; Gill, Ravinder K.; Dudeja, Pradeep K.; Saksena, Seema

doi:10.1152/ajpgi.00210.2015

Cited by 16 publications

(17 citation statements)

References 57 publications

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“…However, the molecular mechanisms involved in the transcriptional modulation of HDPs are not well-understood. The AP-1 transcription factor complex is the main transcription factor downstream of MAPK pathways and can bind with specific DNA sequences (45, 46). In the present study, the AP-1 subunits c-fos and c-jun were activated in IPEC-J2 cells incubated with L. plantarum ZLP001.…”

Section: Discussionmentioning

confidence: 99%

Swine-Derived Probiotic Lactobacillus plantarum Modulates Porcine Intestinal Endogenous Host Defense Peptide Synthesis Through TLR2/MAPK/AP-1 Signaling Pathway

et al. 2019

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Host defense peptides (HDPs) have antimicrobial and immunoregulatory activities and are involved in epithelial innate immune defense. Dietary modulation of endogenous HDP synthesis is an effective way to boost the host innate immune system. This study aimed to investigate the role of the probiotic Lactobacillus plantarum strain ZLP001 in porcine HDP induction and the underlying mechanism. To this end, we evaluated the stimulatory effect of L. plantarum ZLP001 on HDP expression in piglet intestinal tissue in vivo and porcine IPEC-J2 cells and 3D4/31 cells in vitro, and we examined the underlying intracellular signaling pathway in IPEC-J2 cells. L. plantarum ZLP001 treatment increased the mRNA expression of jejunal and ileal HDPs in weaned piglets. In IPEC-J2 and 3D4/31 cells, L. plantarum ZLP001 stimulated HDP expression, but different HDP induction patterns were observed, with the various HDPs exhibiting different relative mRNA levels in each cell line. L. plantarum ZLP001 induced porcine HDP expression through toll-like receptor (TLR)2 recognition as evidenced by the fact that HDP expression was suppressed in TLR2-knockdown IPEC-J2 cells. Furthermore, we found that L. plantarum ZLP001 activated the extracellular signal-regulated kinase (ERK)1/2 and c-jun N-terminal kinase (JNK) signaling pathways, as indicated by enhanced phosphorylation of both ERK1/2 and JNK and the fact that HDP expression was suppressed upon inhibition of ERK1/2 and JNK. Furthermore, L. plantarum ZLP001 activated c-fos and c-jun transcription factor phosphorylation and activity. We conclude that L. plantarum ZLP001 induces porcine HDP expression in vivo and in vitro, and the induction seems to be regulated via TLR2 as well as the ERK1/2/JNK and c-jun/c-fos signaling pathways. Modulation of endogenous HDPs mediated by L. plantarum ZLP001 might be a promising approach to improving intestinal health and enhancing diarrhea resistance in weaning piglets.

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Section: Discussionmentioning

confidence: 99%

Swine-Derived Probiotic Lactobacillus plantarum Modulates Porcine Intestinal Endogenous Host Defense Peptide Synthesis Through TLR2/MAPK/AP-1 Signaling Pathway

et al. 2019

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“…In the last years, numerous efforts were undertaken to demonstrate the health promoting activities of probiotics. Also, some of the mechanisms that leads to the protective functions of probiotics have been suggested (Priyamvada, 2016;Delcenserie, 2008;Yan, 2011). Studies in experimental animals and patients with intestinal inflammation disorders pointed out the potential application of probiotics such as Lactobacillus, Bifidobacteria as immunomodulatory agents (Dieleman, 2003;Gionchetti, 2000;Owczarek, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…It was demonstrated that two widely investigated Lactobacillus sp., L. plantarum and L. gasseri, have the ability to reciprocally modulate IL-10/IL-12 production in cell cultures (Sashihara, 2007). Also, the treatment of intestinal epithelial cells with Lactobacillus acidophillus resulted in the increase of the anti-inflammatory response in epithelial cells by the attenuation of NF-kBmediated inflammatory gene expression (Priyamvada, 2016). Some studies demonstrated that the use of probiotic mixtures might be more effective in reducing gastrointestinal disorders and inflammation than a single strain (Chapman, 2012;Taranu et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A mixture of Lactobacillus sp. modulates the expression of inflammatory molecules, signalling kinases and nuclear receptors in LPS-treated Caco-2 cell culture model

Pistol

Ţăranu

2020

Archiva Zootechnica

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The treatment of intestinal inflammation pathologies (also known as Inflammatory Bowel Diseases, IBD) has included a large variety of strategies, from pharmaceutical to traditional medicine and dietary therapies. In the last years, numerous efforts were undertaken to demonstrate the health promoting activities of probiotics in intestinal inflammation and more other pathologic conditions. The aim of our study was to evaluate the effects of a probiotic mixture of Lactobacillus sp. on the inflammatory mediators and signalling pathways as well as nuclear receptors in colonic Caco-2 cells. Human adenocarcinoma Caco-2 cells were challenged in vitro with lipopolysaccharide (LPS) for 4 hours for the induction of inflammation. The LPS-treated cells were cultured for additional 24 hours in the presence of Lactobacillus (Lb) mixture (3 x108 CFU/mL total Lb). Genomic and proteomic array approaches were used to analyse the profile expression of 18 key genes and their proteins involved in intestinal inflammatory response (chemokines, adhesion molecules, growth factors and matrix metalloproteinases inhibitors) as well as signalling markers (Akt, GSK) and nuclear receptors (NF-kB/RELA, Nrf2, AhR). Our study demonstrated that the probiotic Lactobacillus mixture could decrease LPS-induced inflammatory mediator expressions (chemokines, growth factors and matrix metalloproteinases inhibitor) at gene and protein level. This down-regulation exerted by Lb. mix in LPS-treated Caco-2 cells seemed to be regulated through inhibition of both the PI3K/AKT and NF-κB signalling pathways. Additionally, AhR activation induced by LPS was reduced by probiotic mixture under the level of LPS-treated cells. These beneficial effects of Lactobacillus mixture support their use as inflammatory modulators in intestinal disorders.

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“…Studies with experimental animals and IBD patients pointed out the potential application of probiotics such as lactobacilli, bifidobacteria to prevent or treat colitis (24)(25)(26) . Data from in vitro studies showed that the treatment of intestinal epithelial cells with L. acidophilus resulted in induction of c-Fos and c-Jun proteins via extracellular signal-regulated kinase (Erk)1/2 activation (27) . In addition, probiotic bacteria could increase the antiinflammatory response in epithelial cells by shuttling the transcription factor NF-κB out of the nucleus, which resulted in the attenuation of NF-κB-mediated inflammatory gene expression (27,28) .…”

mentioning

confidence: 99%

Synbiotic combination of prebiotic grape pomace extract and probioticLactobacillussp. reduced important intestinal inflammatory markers and in-depth signalling mediators in lipopolysaccharide-treated Caco-2 cells

2018

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Inflammatory bowel diseases (IBD) are a major problem for public health, with an increased incidence and impact on life quality. The effect of pre- and probiotic combination has been less studied in IBD. Using genomic and proteomic array technologies, this study examined the efficacy of a new combination of natural alternatives: prebiotics (grape pomace extract, GP) and probiotics (lactobacilli mixture, Lb mix) on inflammation and intracellular signalling routes in a cellular model of inflammation. Caco-2 cells challenged with lipopolysaccharide (LPS) for 4 h were treated with GP extract (50 μg/ml gallic acid equivalent) and Lb combination (3 × 108 colony-forming units/ml total Lb) for 24 h. The profile expressions of forty key inflammatory markers and twenty-six signalling kinases were analysed. Other markers involved in inflammation were also investigated (NF-κB/RELA, Nrf2, aryl hydrocarbon receptor, Cyp1A1, Cyp1B1); 57·5 and 60 % of investigated genes and proteins, respectively, were down-regulated by the synbiotic combination. Relevant cytokines and chemokines involved in response to microbial infection and inflammation were reduced under the level induced by LPS treatment and toward the unchallenged control. As expected, the reduction effect seems to imply mitogen-activated protein kinase and NF-κB pathway. Most of the signalling molecules activated by LPS were decreased by GP extract and Lb mix. Our study indicates that the synbiotic combination of GP extract and Lactobacillus sp. mixture exerted anti-inflammatory properties, which are able to decrease the majority of inflammatory genes, their proteins and associated signalling markers. Due to protective role of GP compounds on lactobacilli probiotic, this synbiotic combination might serve as a promising adjunctive therapy in intestinal inflammations.

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Lactobacillus acidophilusstimulates intestinal P-glycoprotein expression via a c-Fos/c-Jun-dependent mechanism in intestinal epithelial cells

Abstract: Priyamvada S, Anbazhagan AN, Kumar A, Soni V, Alrefai WA, Gill RK, Dudeja PK, Saksena S. Lactobacillus acidophilus stimulates intestinal P-glycoprotein expression via a c-Fos/c-Jundependent mechanism in intestinal epithelial cells.

Cited by 16 publications

References 57 publications

Swine-Derived Probiotic Lactobacillus plantarum Modulates Porcine Intestinal Endogenous Host Defense Peptide Synthesis Through TLR2/MAPK/AP-1 Signaling Pathway

Swine-Derived Probiotic Lactobacillus plantarum Modulates Porcine Intestinal Endogenous Host Defense Peptide Synthesis Through TLR2/MAPK/AP-1 Signaling Pathway

A mixture of Lactobacillus sp. modulates the expression of inflammatory molecules, signalling kinases and nuclear receptors in LPS-treated Caco-2 cell culture model

Synbiotic combination of prebiotic grape pomace extract and probioticLactobacillussp. reduced important intestinal inflammatory markers and in-depth signalling mediators in lipopolysaccharide-treated Caco-2 cells

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