Klebsiella pneumoniae haemolysin adsorption to red blood cells

Abstract

It was necessary to incubate the Klebsiella pneumoniae haemolysin with erythrocytes at 37 degrees C to produce the whole lytic action. The amount of attached klebolysin at 4 degrees C increased as its concentration in the medium increased, until the erythrocyte surface was saturated. Treatment with 2-mercaptoethanol was necessary to permit the adsorption; it was inhibited by low concentrations of cholesterol. Klebsolysin was immunogenic and its antiserum neutralized its own haemolytic effect and streptolysin O. Anti-streptolysin serum also neutralized klebsolysin. Streptolysin O attachment to erythrocytes impeded the posterior klebolysin adsorption in the same way that klebsolysin adsorption interfered with streptolysin O attachment.

16SrRNA sequencing analysis for identification of Klebsiella pneumoniae isolated from the extreme kitchen environment

“…16SrRNA sequencing analysis for identification of Klebsiella pneumoniae isolated from the extreme kitchen environment The isolates of Klebsiella pneumoniae obtained from extreme environments differ in their possession of virulence factors. Our isolates could not cause hemolysis of blood, and this result agrees with Pereira and Vanetti 24 may be due to the absence of activators for gene expression that code for this enzyme 25 . In recent research, none of the 54 strains isolated had blood hemolysis or possessed the hlyA and cnf-1 genes, which code for this activity.…”

“…In recent research, none of the 54 strains isolated had blood hemolysis or possessed the hlyA and cnf-1 genes, which code for this activity. Although hemolytic activity has been documented, few studies show the genotypic and phenotypic existence of hemolysis in Klebsiella 25,26 . At the same time, our result differs from Kalaivani and his group 27 , who reported that the Klebsiella pneumonia isolates had a high percentage of hemolytic activity.…”

Potential Virulence of Klebsiella Isolated from Enteral DietsVirulence of Klebsiella and Enteral Diets

“…In a recent study, none of the 54 clinical isolates presented hemolysis or presence of the genes hlyA and cnf-1, which code for this activity. This is consistent with the lack of studies reporting genotypic and phenotypic presence of hemolysis in Klebsiella, even though hemolytic activity has been described [20,21].…”

“…The absence of hemolytic activity under the conditions of this * The isolates are specified in Table 2 and the antibiotics are described in Table 3 Serotyping not determined; serotypes higher than K6 study may be related to a lack of activators for expressing the genes that code for this enzyme, which are often not reproducible under in vitro conditions [20]. This virulence factor was first detected by ALBESA in 1980, in a test conducted on blood agar containing rabbit erythrocytes.…”

Παρουσία μεταλλοενζύμων τύπου VIM-1 σε στελέχη Klebsiella pneumoniae από ασθενείς με μικροβιαιμία

“…Εντούτοις, σε µια µελέτη στελεχών Κ. pneumoniae µε ορότυπο Κ2, τα rmpA-αρνητικά στελέχη ήταν 1000 φορές λιγότερο λοιµογόνα, σε σχέση µε τα wild-type στελέχη, αλλά παρέµεναν 100 φορές περισσότερο λοιµογόνα από τα rmpA και arobactin-αρνητικά στελέχη, που δεν περιείχαν το σχετικό πλασµίδιο 219. Από τα παραπάνω γίνεται εµφανές ότι η aerobactin παίζει άµεσο ρόλο στη λοιµογονικότητα των λίγων στελεχών Κ.pneumoniae που την παράγουν [233][234][235][236]. διάφορες κυτταροτοξίνες[237][238][239][240] και εντεροτοξίνες,[241][242][243][244][245][246] που όµως φαίνεται να παίζουν δευτερεύοντα ρόλο στην λοιµογόνο δράση του βακτηρίου.…”