Inhibition of Shigella sonnei by Lactobacillus casei and Lact. acidophilus

Macias, M. E. Nader De; Apella, María C.; Romero, Nora C.; González, Silvia; Oliver, G.

doi:10.1111/j.1365-2672.1992.tb04996.x

Cited by 51 publications

(4 citation statements)

References 9 publications

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“…Total and anti‐ S. boulardii sIgA in the small intestine contents of gnotobiotic mice was determined by ELISA. The procedure of collecting intestinal contents was described by Nader de Macías et al . (1992) and was modified as follows.…”

Section: Methodsmentioning

confidence: 99%

Saccharomyces boulardiistimulates sIgA production and the phagocytic system of gnotobiotic mice

Rodrigues¹,

Cara²,

Fretez

et al. 2000

Journal of Applied Microbiology

146

120

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The effect of Saccharomyces boulardii on the immune system was evaluated, comparing germ‐free Swiss/NIH mice monoassociated with the probiotic with germ‐free mice. Saccharomyces boulardii colonized the gut of germ‐free mice and survived the gastrointestinal conditions. An increase in sIgA production, both total and anti‐S. boulardii, was observed in the intestinal contents of monoassociated mice when compared with germ‐free controls. The number of Küpffer cells was significantly higher in monoassociated mice than in germ‐free controls. In S. boulardii‐monoassociated mice, clearance of Escherichia coli B41 was higher than in germ‐free controls. TNF‐α, IFN‐γ and IL‐12 serum levels were higher at earlier time points in monoassociated mice when compared with germ‐free mice. These results show that the yeast S. boulardii modulates the host immune responses. This effect may be of interest for improving the resistance to enteropathogenic bacterial infections.

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Section: Methodsmentioning

confidence: 99%

Saccharomyces boulardiistimulates sIgA production and the phagocytic system of gnotobiotic mice

Rodrigues¹,

Cara²,

Fretez

et al. 2000

Journal of Applied Microbiology

146

120

View full text Add to dashboard Cite

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“…1999). There have been reports that feeding of probiotic bacteria prevents Gram‐negative bacterial infections in experimental animals (Nader de Macías et al. 1992; Silva et al.…”

Section: Introductionmentioning

confidence: 99%

Increased resistance of mice to Salmonella enterica serovar Typhimurium infection by synbiotic administration of Bifidobacteria and transgalactosylated oligosaccharides

et al. 2001

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Aims: The anti‐infectious activity of Bifidobacteria in combination with transgalactosylated oligosaccharides (TOS) against Salmonella enterica serovar Typhimurium LT‐2 in an opportunistic antibiotic‐induced murine infection model in mice was examined. Methods and Results: B. breve (strain Yakult) with natural resistance to streptomycin sulphate (SM, MIC: > 4 mg ml–1), when given daily at a dose of 108 cfu/mouse orally under SM treatment was constantly excreted at 1010 cfu g–1 faeces so long as SM was administered, even at 2 weeks after discontinuing administration of B. breve. Explosive intestinal growth and subsequent extra‐intestinal translocation of orally infected LT‐2 under SM treatment were inhibited by B. breve colonization, and this anti‐infectious activity was strengthened by synbiotic administration of TOS with B. breve. Comparison of anti‐Salmonella activity among several Bifidobacterium strains with natural resistance to SM revealed that strains such as B. bifidum ATCC 15696 and B. catenulatum ATCC 27539T conferred no activity, even when they reached high population levels similar those of effective strains such as strain Yakult and B. pseudocatenulatum DSM 20439. Both the increase in the concentration of organic acids and the lowered pH in the intestine due to bifidobacterial colonization correlated with the anti‐infectious activity. Moreover, the crude cecal extract of B. breve‐colonized mice exerted growth‐inhibitory activity against LT‐2 in vitro, whereas that of the ineffective B. bifidum‐colonized cecum showed much lower activity. Conclusions: Intestinal colonization by bifidobacteria given exogenously together with TOS during antibiotic treatment prevents the antibiotic‐induced disruption of colonization resistance to oral infection with S. enterica serovar Typhimurium, and the metabolic activity needed to produce organic acids and lower the intestinal pH is important in the anti‐infectious activity of synbiotics against enteric infection with Salmonella. Significance and Impact of the Study: These results indicate that certain bifidobacteria together with prebiotics may be used for the prophylaxis against opportunistic intestinal infections with antibiotic‐resistant pathogens.

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“…Many of these probiotics are lactic acid bacteria, and anaerobic bifidobacteria have been reported to be useful in the treatment of disturbed intestinal microflora and diarrheal diseases (for a review, see reference 18). Feeding probiotic bifidobacteria to experimental animals has been reported to prevent gram-negative bacterial infections (23,30,32). Some probiotic bifidobacterial strains have been reported to lessen the severity of oral STEC infection in murine experimental infection models.…”

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confidence: 99%

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

et al. 2004

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The anti-infectious activity of probiotic Bifidobacteria against Shiga toxin-producing Escherichia coli (STEC) O157:H7 was examined in a fatal mouse STEC infection model. Stable colonization of the murine intestines was achieved by the oral administration of Bifidobacterium breve strain Yakult (naturally resistant to streptomycin sulfate) as long as the mice were treated with streptomycin in their drinking water (5 mg/ml). The pathogenicity of STEC infection, characterized by marked body weight loss and subsequent death, observed in the infected controls was dramatically inhibited in the B. breve-colonized group. Moreover, Stx production by STEC cells in the intestine was almost completely inhibited in the B. breve-colonized group. A comparison of anti-STEC activity among several Bifidobacterium strains with natural resistance to streptomycin revealed that strains such as Bifidobacterium bifidum ATCC 15696 and Bifidobacterium catenulatum ATCC 27539 T did not confer an anti-infectious activity, despite achieving high population levels similar to those of effective strains, such as B. breve strain Yakult and Bifidobacterium pseudocatenulatum DSM 20439. The effective strains produced a high concentration of acetic acid (56 mM) and lowered the pH of the intestine (to pH 6.75) compared to the infected control group (acetic acid concentration, 28 mM; pH, 7.15); these effects were thought to be related to the anti-infectious activity of these strains because the combination of a high concentration of acetic acid and a low pH was found to inhibit Stx production during STEC growth in vitro.

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Inhibition of Shigella sonnei by Lactobacillus casei and Lact. acidophilus

Cited by 51 publications

References 9 publications

Saccharomyces boulardiistimulates sIgA production and the phagocytic system of gnotobiotic mice

Saccharomyces boulardiistimulates sIgA production and the phagocytic system of gnotobiotic mice

Increased resistance of mice to Salmonella enterica serovar Typhimurium infection by synbiotic administration of Bifidobacteria and transgalactosylated oligosaccharides

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

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