In vivo synthesis of selenium nanoparticles by Halococcus salifodinae BK18 and their anti‐proliferative properties against HeLa cell line

Abstract

Nanoparticles synthesis by bacteria and yeasts has been widely reported, however, synthesis using halophilic archaea is still in a nascent stage. This study aimed at the intracellular synthesis of selenium nanoparticles (SeNPs) by the haloarchaeon Halococcus salifodinae BK18 when grown in the presence of sodium selenite. Crystallographic characterization of SeNPs by X-ray diffraction, Selected area electron diffraction, and transmission electron microscopy exhibited rod shaped nanoparticles with hexagonal crystal lattice, a crystallite domain size of 28 nm and an aspect ratio (length:diameter) of 13:1. Energy disruptive analysis of X-ray analysis confirmed the presence of selenium in the nano-preparation. The nitrate reductase enzyme assay and the inhibitor studies indicated the involvement of NADH-dependent nitrate reductase in SeNPs synthesis and metal tolerance. The SeNPs exhibited good anti-proliferative properties against HeLa cell lines while being non-cytotoxic to normal cell line model HaCat, suggesting the use of these SeNPs as cancer chemotherapeutic agent. This is the first study on selenium nanoparticles synthesis by haloarchaea.

Biosynthesis of silver nanoparticles by the fungus Arthroderma fulvum and its antifungal activity against genera of Candida, Aspergillus and Fusarium

“…Biological methods have become increasingly prominent because they are inexpensive, use mild reaction conditions in a variety of hosts, and can produce stable nanoparticles of controlled dimensions. [2][3][4] Biological methods include synthesis through the use of plants, 5,6 bacteria, 7,8 and fungi. [9][10][11][12] Fungi have become one of the main biological candidates for synthesizing nanoparticles because of their metabolic diversity.…”

Anti-hyperglycemic activity of selenium nanoparticles in streptozotocin-induced diabetic rats

“…10 It also stimulates cell cycle progression and prevents cell death. 6 Furthermore, McNeill et al 11 demonstrated that Se like vanadium appeared to have insulin-like effects when administered in rats and markedly stimulated glucose transport and insulin-sensitive cyclic adenosine monophosphate phosphodiesterase when incubated with rat adipocytes. 12 In this study, we designed new therapeutic strategies for DM which combined SeNPs with insulin in order to investigate whether SeNPs can show antidiabetic activity, or even improve the therapeutic effect in vivo.…”

Anticancer activity of biostabilized selenium nanorods synthesized by Streptomyces bikiniensis strain Ess_amA-1

“…13 Therefore, SNMs are being widely used in basic and applied areas of chemistry, physics, environmental science, material science, and biomedicine. 7,13,17,18 However, concern is now growing regarding the environmental impact of the SNM synthesis process based on physico-chemical methods that require high pressures, temperatures, and toxic chemicals. These methods have some drawbacks: (i) production of stable SNMs dispersions only at lower concentrations and unsuitability for large-scale production; (ii) requirement of additional stabilizing agents; (iii) production of hazardous byproducts, and (iv) increased pollution in the environment.…”

“…6,19,[24][25][26] It is well established that biosynthesized SNMs have several important characteristics, including more stability and higher biological activity, due to the surface functionalization by biological macromolecules secreted by fungi and bacteria. 7,9,17,23,27 Among the different microorganisms, Actinomycetes are less explored for the synthesis of SNMs. Actinomycetes are a diverse group of filamentous true bacteria found in a variety of habitats in terrestrial and aquatic environments.…”

“…24,26,27,60 However, few recent studies reported the biosynthesis of Se nanorods via spherical SeNPs that acted as seeds for growth. 6,17 The high free energy of SeNPs evoked the Ostwald ripening process, which may be responsible for the growth of spherical SeNPs into SeNrs. 17,61 The TEM data also revealed the nucleation/growth of the SeNrs (6 hours and 12 hours incubation) that is probably due to the presence of aromatic amino acids produced by the strain Ess_amA-1, thus indicating possible adhesion of biological macromolecules on the surface of SeNrs.…”