Anticancer activity of biostabilized selenium nanorods synthesized by Streptomyces bikiniensis strain Ess_amA-1

Abstract

Selenium is an important component of human diet and a number of studies have declared its chemopreventive and therapeutic properties against cancer. However, very limited studies have been conducted about the properties of selenium nanostructured materials in comparison to other well-studied selenospecies. Here, we have shown that the anticancer property of biostabilized selenium nanorods (SeNrs) synthesized by applying a novel strain Ess_amA-1 of Streptomyces bikiniensis . The strain was grown aerobically with selenium dioxide and produced stable SeNrs with average particle size of 17 nm. The optical, structural, morphological, elemental, and functional characterizations of the SeNrs were carried out using techniques such as UV-vis spectrophotometry, transmission electron microscopy, energy dispersive X-ray spectrometry, and Fourier transform infrared spectrophotometry, respectively. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that the biosynthesized SeNrs induces cell death of Hep-G2 and MCF-7 human cancer cells. The lethal dose (LD 50% ) of SeNrs on Hep-G2 and MCF-7 cells was recorded at 75.96 μg/mL and 61.86 μg/mL, respectively. It can be concluded that S. bikiniensis strain Ess_amA-1 could be used as renewable bioresources of biosynthesis of anticancer SeNrs. A hypothetical mechanism for anticancer activity of SeNrs is also proposed.

Specificity of Biogenic Selenium Nanoparticles for Prostate Cancer Therapy With Reduced Risk of Toxicity: An in vitro and in vivo Study

“…Reports on chemically synthesized SeNP suggested lower toxicity and equivalent anticancer activity compared to inorganic and organic selenium compounds (3,11,21,22). Similarly, biogenic SeNPs synthesized by Acinetobacter, Streptomyces, and Halococcus also showed anticancer activity, however, at higher doses (13)(14)(15). Earlier in our study, we have shown that a concentration of 2 µg Se/ml of our sterically stabilized biogenic SeNPs is very effective in inducing TNF/IRF1 mediated necroptosis in PC-3 cells without compromising the viability of human peripheral blood mononuclear cell (hPBMC) (18,19).…”

“…The greater biocompatibility and better stability of these particles are reported to be due to the differential nanostructural arrangements of selenium atoms (12). Some biogenic SeNPs are also reported to induce cell death in various cancer cell lines including Hep-G2, MCF-7, 4T1, and HeLa (13)(14)(15). However, their effect was observed at higher doses.…”

Microbial Nanotechnology: Challenges and Prospects for Green Biocatalytic Synthesis of Nanoscale Materials for Sensoristic and Biomedical Applications

“…In the last ten years, bacteria have been used to synthesize inorganic nanomaterials (mainly selenium, gold, and silver nanoparticles) with interesting properties for the development of voltammetric sensoristic devices [19], and third-generation biosensors [20], for possible diagnostic applications [21] like cell imaging and biolabeling [22] and for applications where no surface coat is required, like annealing and thin film formation [23] (Table 1). Bacterial-biosynthesized nanoparticles have mainly shown in vitro antimicrobial activity against some pathogenic bacterial strains [24][25][26][27][28] and properties i.e., antioxidant [29], anti-proliferative, anti-migration [30], anticoagulant [31], and anticancer [26][27][28][29][30][31][32][33]. Biochemical mechanisms which mediate the bacterial biosynthesis of nanoparticles have been proposed or they are currently under investigation.…”

Microbial-Based Bioremediation of Selenium and Tellurium Compounds

“…Both Se-and Te-nanomaterials resulted efficient tools in protecting living organisms from DNA oxidation [181], as well as promising antimicrobial and anticancer agents [182][183][184][185][186][187].…”